We narrowed to 7,014 results for: tac

Showing: 5201 - 5220 of 7014 results

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Lenti-FAB(mito-LD)

Plasmid

#214421

Purpose

Detection of mito-lipid droplet contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspAN-3xHA-V5-mito_IRES_CFAST-20nm-Halo-6xHp

Use

Lentiviral

Tags

CFAST-Halo and RspAN-3XHA-V5

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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CanlonicSF

Plasmid

#178342

Purpose

To explore lactate dynamics in intact systems

Depositor

Alejandro San Martín

Article

Aburto et al ACS Sens. 2022 Oct 28. doi: 10.1021/acss

Insert

CanlonicSF

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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shPBRM1#32-Tet-pLKO-puro

Plasmid

#107399

Purpose

Lentiviral expression of shRNA targeting PBRM

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

shRNA targeting PBRM1 (PBRM1 Human)

Use

Lentiviral

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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shPBRM1#131-Tet-pLKO-puro

Plasmid

#107400

Purpose

Lentiviral expression of shRNA targeting PBRM

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

shRNA targeting PBRM1 (PBRM1 Human)

Use

Lentiviral

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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MAC_N_GATA4

Plasmid

#167758

Purpose

Expresses GATA4 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

GATA4 (GATA4 Human)

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-FAB(ER-mito)

Plasmid

#214423

Purpose

Detection of ER-mito contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspAN-FRB-V5-Sec61_IRES_CFAST10-20nm-Halo-mito

Use

Lentiviral

Tags

CFAST10-Halo and RspAN-FRB-V5

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pSR348

Plasmid

#124713

Purpose

Expression of narX under Ptac

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

narX (narX )
lacI (lacI )

Use

Synthetic Biology

Expression

Bacterial

Mutation

S193* - LacI is truncated and likely deactivated.…

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pMBP-HRV3C-AcrIF9

Plasmid

#141442

Purpose

Expression of AcrIF9 in E. coli, tagged with an HRV3C-cleavage fusion to Maltose Binding Protein.

Depositor

Blake Wiedenheft

Article

Hirschi et al Nat Commun. 2020 Jun 1;11(1):2730. doi:

Insert

AcrIF9

Tags

HRV3C cleavage site and Maltose Binding Protein

Expression

Bacterial

Promoter

ptac

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-MC38-TCRB-32-riot_punisher

Plasmid

#138988

Purpose

IVT template for the beta subunit of the mouse TCR #32 that is reactive against MC38

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRB (Tcrb Mouse)

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-MC38-TCRB-31-murderous_crow

Plasmid

#138986

Purpose

IVT template for the beta subunit of the mouse TCR #31 that is reactive against MC38

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRB (Tcrb Mouse)

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pMK312 (TOP2A CRISPR)

Plasmid

#140654

Purpose

TOP2A tagging CRISPR

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

TOP2A (TOP2A Human)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti-FAB(ER-LD)

Plasmid

#214420

Purpose

Detection of ER-lipid droplet contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspAN-FRB-V5-ER_IRES_CFAST-20nm-Halo-6xHp

Use

Lentiviral

Tags

CFAST-Halo and RspAN-FRB-V5

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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NAE1 G8.3 gRNA

Plasmid

#90777

Purpose

3rd generation lentiviral gRNA plasmid targeting human NAE1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

NAE1 (Guide Designation G8.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2_mSTING_scrambled_gRNA_2

Plasmid

#196628

Purpose

Scrambled control 2 for STING knock-out in murine cells.

Depositor

Nicolas Manel

Article

Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

Insert

mSTING scrambled gRNA 2

Use

CRISPR and Lentiviral

Mutation

WT

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPEY-GAL-Kan-ADE2

Plasmid

#232105

Purpose

Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

Depositor

Jeffrey Lewis

Article

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Insert

ADE2 gRNA and repair template

Use

CRISPR

Expression

Yeast

Mutation

Repair template introduces C at nt 466 of ADE2 to…

Promoter

GAL7

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA3 V5 mDispatched WT 2x HA

Plasmid

#126410

Purpose

Expression of V5 Dispatched WT HA in mammalian cell lines.

Depositor

Stacey Ogden

Article

Stewart et al Elife. 2018 Jan 23;7. pii: 31678. doi: 1

Insert

m-Dispatched1 (Disp1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

V5 iserted between A106 and A107

Available Since

June 14, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a-LCB1_v1.3-PDGFR-T2A-mCherry (pZYW048)

Plasmid

#194231

Purpose

Lentiviral expression vector expressing LCB1 on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction marker

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

EF1a-LCB1-PDGFR TMD-T2A-mCherry

Use

Lentiviral

Promoter

EF1a

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO-puro-tetON-shRFP

Plasmid

#110940

Purpose

Tet-inducible shRNA targeting RFP

Depositor

William Hahn

Article

Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

Insert

shRFP

Use

Lentiviral and RNAi

Promoter

H1/TO

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-LiMETER-v1

Plasmid

#113933

Purpose

Express an optogenetic tether to label and interrogate ER-plasma membrane contact sites (MCS); visualized by GFP

Depositor

Yubin Zhou

Article

Jing et al Nat Cell Biol. 2015 Oct;17(10):1339-47.

Insert

LOV2 and Rit polybasic domain (PB)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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Lenti-sgRb1/Cre

Plasmid

#89647

Purpose

Expresses an Rb1-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting Rb1 (Rb1 Mouse)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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