We narrowed to 10,414 results for: 158

1. pcs2-HRI-GFP-IRES-mCherry

   Plasmid

   #226099

   Purpose

   HRI stability reporter construct for transient expression in mammalian cells

   Depositor

   Michael Rape

   Article

   Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

   Insert

   HRI (EIF2AK1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLenti-Puro-sgNUAK1

   Plasmid

   #138692

   Purpose

   Expresses a human NUAK1-targeting sgRNA and Cas9

   Depositor

   Reuben Shaw

   Article

   Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

   Insert

   sgNUAK1 human (NUAK1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. eTd-CBE

   Plasmid

   #196601

   Purpose

   Mammalian expression of eTd-CBE

   Depositor

   Dali Li

   Article

   Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

   Insert

   bpNLS-TadA-8e (N46L)-nSpCas9-bpNLS-P2A-UGI-bpNLS
   

   Use

   Tags

   6*His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHS2 (hANGPTL4-V5)

   Plasmid

   #102446

   Purpose

   Expresses V5-tagged human ANGPTL4 in mammalian cells

   Depositor

   Brandon Davies

   Article

   Chi et al J Biol Chem. 2015 May 8;290(19):11865-77

   Insert

   ANGPTL4 (ANGPTL4 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-mCherry-sesRNA-smFlag-tTA2-W3SL

   Plasmid

   #192066

   Purpose

   Expression of mCherry, sesRNA in mammalian cells, with smFlag and tTA2 as efRNA.

   Depositor

   Josh Huang

   Article

   Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

   Insert

   sesRNA for Mouse Ctip2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLCHKO_HPRT1_exon2_1_Lb

   Plasmid

   #155051

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and LbCas12a nucleases (CHyMErA system)

   Depositor

   Jason Moffat, Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of HPRT1 exon 2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLCHKO_HPRT1_exon3_1_Lb

   Plasmid

   #155053

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and LbCas12a nucleases (CHyMErA system)

   Depositor

   Jason Moffat, Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of HPRT1 exon 3
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti CMV SNAP MDC1

   Plasmid

   #227375

   Purpose

   SNAP–MDC1 expression

   Depositor

   David Pellman

   Article

   Papathanasiou et al Nature. 2023 Jul;619(7968):184-192. doi:

   Insert

   MDC1, mediator of DNA damage checkpoint 1 (MDC1 Human)
   

   Use

   Lentiviral

   Tags

   SNAP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. SpCas9-NRCH-ABE8e(V106W)

   Plasmid

   #198556

   Purpose

   Expresses human codon-optimized SpCas9-NRCH-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-NRCH-ABE8e(V106W)-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NRCH-ABE8e(V106W)
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLenti-Puro-sgNUAK2

    Plasmid

    #138693

    Purpose

    Expresses a human NUAK2-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgNUAK2 human (NUAK2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MSI2-DCD

    Plasmid

    #166968

    Purpose

    Express the fusion protein of human MSI2 and the catalytic dead domain of Drosophila ADAR

    Depositor

    Michael Kharas

    Article

    Nguyen et al Nat Commun. 2020 Apr 24;11(1):2026. doi:

    Insert

    MSI2-DCD
    

    Use

    Retroviral

    Tags

    MSI2-DCD

    Expression

    Mammalian

    Mutation

    N495S

    Promoter

    MSCV

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV.Ple155-CI-mRuby2-W3SL_BbsI(GGA)

    Plasmid

    #231364

    Purpose

    Ple155-driven mRuby2, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    mRuby2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MSI2-ADA

    Plasmid

    #166967

    Purpose

    Express the fusion protein of human MSI2 and the hyperactive catalytic domain of Drosophila ADAR

    Depositor

    Michael Kharas

    Article

    Nguyen et al Nat Commun. 2020 Apr 24;11(1):2026. doi:

    Insert

    MSI2-ADA
    

    Use

    Retroviral

    Tags

    MSI2-ADA

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. EGFP-COV M

    Plasmid

    #165124

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    COV M (M codon optimized SARS-COV-2)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-HaloEKAR2.2-T2A-EGFP

    Plasmid

    #216495

    Purpose

    Expression of chemigenetic ERK biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

    Depositor

    Jin Zhang

    Article

    Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

    Insert

    HaloEKAR2.2
    

    Use

    Tags

    T2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pIRESpuro-Flag-Pol b

    Plasmid

    #23257

    Depositor

    Robert Sobol

    Article

    Tang et al Mol Cancer Res. 2010 Jan;8(1):67-79. doi

    Insert

    DNA polymerase beta (POLB Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pKH151_PB_flag-LC3B_wt_IRES-BFP

    Plasmid

    #214976

    Purpose

    Flag tagged LC3B

    Depositor

    Wade Harper

    Article

    Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

    Insert

    FLAG-LC3B (MAP1LC3B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. A*11:01:01

    Plasmid

    #203281

    Purpose

    Overexpress HLA allele

    Depositor

    Catherine Wu, Derin Keskin

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    A*11:01:01 (HLA-A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-FLAG-MTOR-C1483F

    Plasmid

    #69008

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-C1483F (MTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Cysteine 1483 to Phenylalanine

    Promoter

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pCI-neo-Flag-DHHS

    Plasmid

    #85813

    Purpose

    To rescue the expression of DHHC5 catalytically inactive mutant in the knockdown cell lines

    Depositor

    Sandra Hofmann

    Article

    Tian et al Mol Cancer Res. 2015 Apr;13(4):784-94. d

    Insert

    ZDHHC5 C134S mutant (Zdhhc5 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pKH152_PB_flag-LC3B_LDSmut_IRES-BFP

    Plasmid

    #214977

    Purpose

    Flag tagged LDS mutant LC3B

    Depositor

    Wade Harper

    Article

    Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

    Insert

    FLAG-LC3B_mut (MAP1LC3B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    LDS mutant

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pFS_0414_pET30_MCS-BBaJ23100-LuxR-B0015term-pLux rR-Fluc-rrnBterm-pCat-tetR-Term-ptetA-Rluc-rrnBterm_FsRT-Cas1-Cas2_ARRAY2_FaqI

    Plasmid

    #117005

    Purpose

    Expresses FsRT-Cas1-Cas2 under pT7lac, Fluc under pLuxR and Rluc under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV_CAG_FLEX_PKIα-P2A-mRuby3_WPRE

    Plasmid

    #196677

    Purpose

    AAV plasmid for mammalian Cre-dependent expression of PKIα-P2A-mRuby3 under a CAG promotor w/ WPRE enhancer. PKIα (protein kinase inhibitor α) inhibits the activity of protein kinase A (PKA).

    Depositor

    Haining Zhong

    Article

    Ma et al Nature. 2022 Nov;611(7937):762-768. doi:

    Insert

    PKIα-P2A-mRuby3
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. A*02:02:01:01

    Plasmid

    #203277

    Purpose

    Overexpress HLA allele

    Depositor

    Catherine Wu, Derin Keskin

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    A*02:02:01:01 (HLA-A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA -CLCN3-3xFlag

    Plasmid

    #225506

    Purpose

    CLCN3 expression vector

    Depositor

    Wade Harper

    Article

    Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

    Insert

    CLCN3 (CLCN3 Human)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET-His6MBP-A3A-Gly6His6

    Plasmid

    #187822

    Purpose

    Expression human APOBEC3A in E.coli.

    Depositor

    Hao Wu

    Article

    Fabyanic et al Nat Biotechnol. 2023 Aug 28. doi: 10.103

    Insert

    APOBEC3A (APOBEC3A Human)
    

    Use

    CRISPR

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7 promoter (on the backbone)

    Available since

    July 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSL5069 (pDonor(AAVS1),CRISPR_Pse)

    Plasmid

    #200904

    Purpose

    Encodes a mini-transposon derived from Pseudoalteromonas Tn7016 with a splice acceptor-T2A-PuroR-T2A-eGFP cargo, and a CRISPR RNA under a U6 promoter. Total transposon size = 2,024 bp.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    Mini Tn7016 (AAVS1 NGS), PseCRISPR (tSL425)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV.minBG-EGFP-W3SL_2x-U6-sasgAi14

    Plasmid

    #231365

    Purpose

    minBG-driven EGFP, also encoding U6-driven sgRNAs to direct saCas9 to both sides of stop cassette in Ai14 mice.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV.Ple155-CI-EGFP-W3SL_2x-U6-sasgAi14

    Plasmid

    #231366

    Purpose

    Ple155-driven EGFP, also encoding U6-driven sgRNAs to direct saCas9 to both sides of stop cassette in Ai14 mice.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV.minCMVe-SCP1-SaCas9-W3SynpA

    Plasmid

    #231370

    Purpose

    SCP1 promoter-driven SaCas9 with truncated CMV enhancer and short synthetic terminator sequence.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    SaCas9
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge