We narrowed to 32,197 results for: crispr

1. pCas2B

   Plasmid

   #82373

   Purpose

   dCas9 expressed from aTc-inducible promoter in acsA, B RBS: TCGAGA

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   dCas9
   

   Promoter

   PEZ3

   Available Since

   Oct. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCas8

   Plasmid

   #82380

   Purpose

   dCas9 without a promoter in acsA

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   dCas9
   

   Promoter

   none

   Available Since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCas7

   Plasmid

   #82379

   Purpose

   dCas9 expressed from low constituve promoter, c225, in acsA

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   dCas9
   

   Promoter

   c225

   Available Since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCas2E

   Plasmid

   #82376

   Purpose

   dCas9 expressed from aTc-inducible promoter in acsA, E RBS: AGGGCG

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   dCas9
   

   Promoter

   PEZ3

   Available Since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCas2A

   Plasmid

   #82372

   Purpose

   dCas9 expressed from aTc-inducible promoter in acsA, A RBS: AGGAGA

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   dCas9
   

   Promoter

   PEZ3

   Available Since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCas28

   Plasmid

   #82385

   Purpose

   sgRNA targeting YFP expressed from pJ23108 in the NS1 locus with kanamycin resistance

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   gRNA
   

   Promoter

   PJ23108

   Available Since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCas27

   Plasmid

   #82384

   Purpose

   sgRNA targeting YFP expressed from pJ23117 in the NS1 locus with kanamycin resistance

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   gRNA
   

   Promoter

   PJ23117

   Available Since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. MsMUT_sgRNA1

   Plasmid

   #111296

   Purpose

   CRISPR KO murine MUT

   Depositor

   Vamsi Mootha

   Article

   Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

   Insert

   sgRNA1 against murine MUT
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Availability

   Academic Institutions and Nonprofits only

   

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9. MsMUT_sgRNA3

   Plasmid

   #111297

   Purpose

   CRISPR KO murine MUT

   Depositor

   Vamsi Mootha

   Article

   Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

   Insert

   sgRNA3 against murine MUT
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGMC00018 (aka pRC0082)

    Plasmid

    #195320

    Purpose

    Minimal plasmid backbone with multiple cloning site for HDR donor DNA construction

    Depositor

    Raj Chari

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial and Mammalian

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGMC00026 (aka pRC0462)

    Plasmid

    #195318

    Purpose

    Lentiviral guide RNA backbone derived from pSB700 with neomycin/G418

    Depositor

    Raj Chari

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. CMV-CASANOVA

    Plasmid

    #113035

    Purpose

    CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. FASTHDR-tRNApromo-sgRNA-espCas91_1

    Plasmid

    #167210

    Purpose

    Plasmid for easy cloning of a sgRNA sequence. The Plasmid contain a tRNA promoter to facilitate the expression of any sgRNA sequence and also expresses eSpCas9(1.1)

    Depositor

    Oscar Perez

    Article

    Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT3TS nErCas12an

    Plasmid

    #132642

    Purpose

    Used for in vitro transcription of dual NLS ErCas12a mRNA

    Depositor

    Stephen Ekker

    Article

    Wierson et al CRISPR J. 2019 Dec;2(6):417-433. doi: 10

    Insert

    ErCas12a
    

    Use

    CRISPR; In vitro transcription

    Tags

    SV40 NLS

    Promoter

    T3

    Available Since

    Nov. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR-EF1a-dCas9-HA-BFP-KRAB-NLS

    Plasmid

    #102244

    Purpose

    Lentiviral expression of dCas9-HA-BFP-KRAB-NLS for CRISPRi driven by a hEF1alpha promoter

    Depositor

    Jacob Corn

    Insert

    dCas9-HA-BFP-KRAB-NLS
    

    Use

    CRISPR and Lentiviral

    Tags

    HA-TagBFP-KRAB-NLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. PX408 Francisella tularensis subsp. novicida Cas9

    Plasmid

    #68705

    Purpose

    Francisella tularensis subsp. novicida Cas9

    Depositor

    Feng Zhang

    Insert

    FtCas9
    

    Use

    CRISPR

    Tags

    HA-NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. p-dCas9-dTET1-H1672Y-D1674A-Hygro

    Plasmid

    #104413

    Purpose

    transient expression of dCas9-dTET1 fusion protein

    Depositor

    Stephan Beck

    Insert

    dTET1
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    H1672Y-D1674A

    Promoter

    CMV promoter

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. PX400 Campylobacter lari CF89-12 Cas9

    Plasmid

    #68336

    Purpose

    Campylobacter lari CF89-12 Cas9

    Depositor

    Feng Zhang

    Insert

    ClCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCB32

    Plasmid

    #111911

    Purpose

    Expresses gRNA to target Nte1 locus and has HygR marker

    Depositor

    Yi Tang

    Insert

    gRNA cassette targeting Nte1 locus
    

    Use

    CRISPR

    Expression

    Bacterial and Yeast

    Promoter

    SNR52p

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. p-dCas9-JMJD2A-S288G-T289G-Hygro

    Plasmid

    #104418

    Purpose

    transient expression of dCas9-dJMJD2A fusion protein

    Depositor

    Stephan Beck

    Insert

    dJMJD2A
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    S288G-T289G

    Promoter

    CMV promoter

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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