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pDEST40-DJ1-C106A

Plasmid

#29409

Depositor

Mark Cookson

Insert

DJ1 (PARK7 Human)

Use

Gateway Cloning

Tags

6XHis and V5

Expression

Mammalian

Available Since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
PT7lacO-EF-Tu-His8

Plasmid

#224370

Purpose

Express EF-Tu protein for his-tagged purification

Depositor

Richard Murray

Article

Zhang et al ACS Synth Biol. 2025 May 16;14(5):1496-1

Insert

EF-Tu (tufB E. coli)

Tags

His8

Expression

Bacterial

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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LbCas12a-crRNA

Plasmid

#213753

Purpose

For circular RNA-mediated prime editor using LbCas12a-crRNA in HEK293T cells

Depositor

Caixia Gao

Article

Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Insert

LbCas12a-crRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pYS16

Plasmid

#202082

Purpose

Parent vector for producing universal minicircle donors for mNeonGreen C-terminal knock-in

Depositor

Daniel Dickinson

Article

Shi et al Development. 2023 May 15;150(10):dev2013

Insert

CT mNG-T2A-PuroR Minicircle Parent

Use

CRISPR

Tags

mNeonGreen

Promoter

None

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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hKCNQ3(WT).ires.mCherry-pcDNA3

Plasmid

#204362

Purpose

Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

Depositor

Aguan Wei

Article

Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

Insert

KCNQ3 (KCNQ3 Human)

Expression

Mammalian

Promoter

CMV/T7 RNApol

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_HA1m

Plasmid

#190474

Purpose

Express the medium-affinity histamine sensor GRAB_HA1m in neurons

Depositor

Yulong Li

Article

Dong et al Neuron. 2023 Mar 7:S0896-6273(23)00128-9

Insert

Histamine sensor GRAB_HA1m (medium affinity)

Use

AAV

Promoter

hSyn

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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EYFP Peroxisome visual barcode

Plasmid

#158677

Purpose

tagging clonal populations with Peroxisomal EYFP fluorescent protein. Adding Peroxisome localization signal to EYFP WC

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

EYFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-UXPS

Plasmid

#172136

Purpose

mammalian expression plasmid for a UDP/UTP sensor

Depositor

Huiwang Ai

Article

Li et al ACS Cent Sci. 2021 Oct 27;7(10):1763-177

Insert

UXPS

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_eCBmut

Plasmid

#164605

Purpose

Express the endocannabinoid non-responsive sensor GRAB_eCBmut in neurons

Depositor

Yulong Li

Article

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid non-responsive sensor GRAB_eCBmut

Use

AAV

Mutation

S383T, F177A

Promoter

hSyn

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV_eA3A-nCas9

Plasmid

#163535

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

eA3A-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pLV_mTurquoise2-iLID-CAAX

Plasmid

#160999

Purpose

Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via C-terminal fusion to CAAX motif from KRAS

Depositor

Sean R Collins

Article

Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

Insert

mTurquoise2-iLID-CAAX

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-SYN_mCyRFP2-CREB

Plasmid

#137008

Purpose

AAV backbone, mCyRFP2-CREB donor for R-CREB

Depositor

Ryohei Yasuda

Article

Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

Insert

CREB1 (Creb1 Rat)

Use

AAV

Tags

mCyRFP2

Expression

Mammalian

Promoter

hSynapsin

Available Since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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pET15b T7pCONS sfGFP

Plasmid

#154467

Purpose

pET15b with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFP

Depositor

Daniel Daley

Article

Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

Insert

super folder GFP

Tags

His-thrombin

Expression

Bacterial

Promoter

T7

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pEF-APEX2-NLS

Plasmid

#140281

Purpose

Expression of APEX2-NLS

Depositor

Yusuke Miyanari

Article

Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

Insert

APEX2-NLS

Expression

Mammalian

Promoter

EF1a

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF3_P2A_Hygro_Barcode

Plasmid

#120490

Purpose

Barcoded lentiviral vector to express KLF3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF3 (KLF3 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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FUW-hR3-EGFP

Plasmid

#119231

Purpose

Plasmid encodes full-length human VEGFR-3 gene fused in-frame with EGFP cloned in lentiviral vector.

Depositor

Kari Alitalo

Insert

VEGFR3-EGFP (FLT4 Human)

Use

Lentiviral

Tags

EGFP

Promoter

P hUbC

Available Since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)_NADP-Snifit

Plasmid

#113913

Purpose

Plasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+

Depositor

Kai Johnsson

Article

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Insert

NADP-Snifit

Expression

Bacterial

Promoter

T7

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pEM705 Gs15

Plasmid

#109360

Purpose

GsX chimera with Gα15 C-terminus

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Gs15 chimera

Tags

None

Expression

Mammalian

Promoter

CAG

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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pLJM1-EGFP-BarcodeV2

Plasmid

#78632

Purpose

GESTALT V2 integrating barcode with Puro and EGFP

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

GESTALT V2 insert

Use

Lentiviral

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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PT7-deGFP-MGapt

Plasmid

#67741

Purpose

T7 promoter expressing deGFP-MG aptamer fusion

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

UTR1-deGFP-MGapt-T7term

Use

Synthetic Biology

Expression

Bacterial

Promoter

PT7

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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