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pLKO.1-shMBNL1-9155

Plasmid

#115460

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shMBNL1-9155 (MBNL1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pFRT-TO-RPB1-His K1278R CRres si2,4R

Plasmid

#139406

Purpose

Dox-inducible, K1278R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

Depositor

Jesper Svejstrup

Article

Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

Insert

RPB1 (POLR2A Human)

Tags

6xHis

Expression

Mammalian

Mutation

K1278R

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pMTS_mScarlet-H_N1

Plasmid

#85058

Purpose

In vivo visualization of the mitochondria (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

MTS (COX8A Human)

Tags

mScarlet-H

Expression

Mammalian

Mutation

aa1-29

Promoter

CMV

Available Since

March 16, 2017

Availability

Academic Institutions and Nonprofits only

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pTE4495

Plasmid

#80338

Purpose

mammalian expression MbCpf1 nuclease and Mb crRNA

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Inserts

Mb crRNA
MbCpf1

Use

CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Promoter

CMV and human U6

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-BRCA1 3'UTR

Plasmid

#29477

Depositor

Judy Lieberman

Article

Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

Insert

BRCA1 3'UTR (BRCA1 Human)

Expression

Mammalian

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
G31, Cdc20-AID tagging

Plasmid

#130269

Purpose

Tagging the Saccharomyces cerevisiae Cdc20 gene with a auxin-degron tag, via homologous recombination

Depositor

Matthias Heinemann

Article

Ozsezen et al Cell Syst. 2019 Oct 4. pii: S2405-4712(1

Insert

Cdc20/AID(71-114)-tCYC1-NatMX (CDC20 Budding Yeast, Synthetic)

Tags

AID(71-114)

Expression

Yeast

Promoter

Native promoter

Available Since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pLV EF1A mSOG1-94-FRB-P2A-T2A-FKBP-mSOG-Ja95-140

Plasmid

#136601

Purpose

Lentiviral vector encoding mSOG1-94-FRB-P2A-T2A-FKBP-mSOG-Ja95-140 under the EF1A promoter.

Depositor

John Ngo

Article

Boassa et al Cell Chem Biol. 2019 Oct 17;26(10):1407-

Insert

N-mSOG-FRB-P2A-T2A-FKBP-C-mSOG

Use

Lentiviral

Expression

Mammalian

Promoter

EF1A

Available Since

May 6, 2021

Availability

Academic Institutions and Nonprofits only

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EPHB4-2A-EGFP

Plasmid

#139968

Purpose

Donor vector to knockin EGFP (separated by 2A) into EPHB4 allele

Depositor

James Thomson

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jul 25;11

Insert

Human EPHB4 knockin homology arms (EPHB4 Human)

Expression

Mammalian

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-RAB10 K154A

Plasmid

#130886

Purpose

Expresses human RAB10 K154A mutant with N-terminal GFP tag

Depositor

Michael Bassik

Article

Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

Insert

RAB10 (RAB10 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

K154A

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shMBNL1-3964

Plasmid

#115459

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shMBNL1-3964 (MBNL1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-CDreV

Plasmid

#140133

Purpose

can be used to generate AAV virus that will express fusion protein of split Dre (C-terminal) and fungal photoreceptor Vivid (VVD) monomer

Depositor

Hongkui Zeng

Article

Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

Insert

CDreV

Use

AAV

Promoter

EF1a

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 hSlp2-a C2AB

Plasmid

#40051

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp2-a C2AB (SYTL2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

C2AB domains only (aa 597-910)

Promoter

CMV promoter

Available Since

Oct. 19, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-C1 hSlp2-a del.SHD

Plasmid

#40050

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp2-a del.SHD (SYTL2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

deletion of SHD domain (1-56)

Promoter

CMV promoter

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP Kind2 S159/181/666A

Plasmid

#105310

Purpose

To study altered downstream functions of phospholylation-site null kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S159/181/666A

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pFRT-TO-RPB1-His K710,719R CRres si2,4R

Plasmid

#139407

Purpose

Dox-inducible, K710, 719R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

Depositor

Jesper Svejstrup

Article

Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

Insert

RPB1 (POLR2A Human)

Tags

6xHis

Expression

Mammalian

Mutation

K710,719R

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shMBNL3-0455

Plasmid

#115463

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shMBNL3-0455 (MBNL3 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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G30, Cdc20-mCherry-AID tagging

Plasmid

#130270

Purpose

Tagging the Saccharomyces cerevisiae Cdc20 gene with a mCherry and auxin-degron tag, via homologous recombination

Depositor

Matthias Heinemann

Article

Ozsezen et al Cell Syst. 2019 Oct 4. pii: S2405-4712(1

Insert

Cdc20/mCherry-AID(71-114)-tCYC1-NatMX (CDC20 Budding Yeast, Synthetic)

Tags

AID(71-114) and mCherry

Expression

Yeast

Promoter

Native promoter

Available Since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pX330-Esrrb gRNA

Plasmid

#128841

Purpose

gRNA for targeting mouse Esrrb locus using CRISPR-cas technique

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

Insert

Esrrb gRNA (Esrrb Mouse)

Use

CRISPR

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 hSlp4-a C2AB

Plasmid

#40037

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a C2AB (SYTL4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

C2A and C2B domains (353-671)

Promoter

CMV promoter

Available Since

Oct. 17, 2012

Availability

Academic Institutions and Nonprofits only

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Lenti-sgZmat3.55.9/Cre

Plasmid

#167853

Purpose

Expresses Cre recombinase and an sgRNA targeting Zmat3

Depositor

Laura Attardi

Article

Bieging-Rolett et al Mol Cell. 2020 Nov 5;80(3):452-469.e9. d

Insert

sgRNA targeting Zmat3

Use

CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

Expression

Mammalian

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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