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pGGGSx4

Plasmid

#165807

Purpose

Flexible peptide linker of 4 repeats of GGGS, for assembly of TL1 to TL5 parts

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

GGGSx4

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pT2A

Plasmid

#165809

Purpose

Viral T2A linker element for assembly of TL3 to TL5 parts

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

T2A

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pGGSx4 NT

Plasmid

#165810

Purpose

Flexible peptide linker of 4 repeats of GGS, for assembly of N-terminal TL1 tags to TL3 parts

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

GGSx4 NT

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pR20A02

Plasmid

#165791

Purpose

GB2.0 compatible FlyLight Enhancer element R20A02 in enhancer grammar

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

R20A02

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pR70B04

Plasmid

#165792

Purpose

GB2.0 compatible FlyLight Enhancer element R70B04 in enhancer grammar

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

R70B04

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pR76H03

Plasmid

#165793

Purpose

GB2.0 compatible FlyLight Enhancer element R76H03in enhancer grammar

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

R76H03

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCP6-Dros

Plasmid

#165794

Purpose

Fusion grammar bacterial CP6 promoter, assembles with pHSP70B-TACT

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

CP6 Promoter

Expression

Bacterial

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCP7-Dros

Plasmid

#165796

Purpose

Fusion grammar bacterial CP7 promoter, assembles with pHSP70B-TACT

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

CP7 promoter

Expression

Bacterial

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pdSCP

Plasmid

#165776

Purpose

Minimal synthetic Drosophila core promoter, requires enhancer element for full expression

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

dSCP

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pAra-tns

Plasmid

#170632

Purpose

Expression of CRISPR-associated transposases

Depositor

Sheng Yang

Article

Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

Insert

VchTnsABC

Expression

Bacterial

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pmCherry-NT-Tagging

Plasmid

#165870

Purpose

Template for amplification of mCherry N-terminal tagging cassette

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Vector - NT Tagging

Use

Synthetic Biology

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pVG97

Plasmid

#164849

Purpose

Plasmid for replacement of PEST sequence in strains with pr-yEVenus-PEST-ADH1t construct

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

Insert

Part of URA3 5' UTR and yEVenus with stop codon for recombination with pr-yEVenus-PEST-ADH1t:URA

Expression

Yeast

Available Since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pDest-639 (attR4-attR2)

Plasmid

#161880

Purpose

Multisite Gateway (attR4-attR2) vector for expression of multiple proteins using the Bac-to-Bac insect cell system

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Expression

Insect

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pDest-624 (attR4-attR2)

Plasmid

#161879

Purpose

Multisite Gateway (attR4-attR2) vector for expression of multiple proteins using the Bac-to-Bac insect cell system

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Expression

Insect

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pAG369-lyn11-redFAST

Plasmid

#160356

Purpose

Expresses Lyn11-redFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

lyn11-redFAST

Expression

Mammalian

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pAG361-lyn11-greenFAST

Plasmid

#160352

Purpose

Expresses Lyn11-greenFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

lyn11-greenFAST

Expression

Mammalian

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pPB-VASA-tdTomato-pNeo

Plasmid

#141030

Purpose

Reporter integration

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

tdTomato

Expression

Mammalian

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pBz118_His_CsoS2

Plasmid

#140841

Purpose

Halothiobacillus neapolitanus CsoS2 with N-terminal His-tag, pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2

Tags

Hexahistidine tag

Expression

Bacterial

Promoter

T7

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-L1-U6-BsmBI-R5

Plasmid

#141032

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Expression

Mammalian

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pUC-DEST-cjSTELLA-mCerulean-PuroTK

Plasmid

#141029

Purpose

Reporter integration

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

mCerulean

Expression

Mammalian

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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