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pTRANS_212

Plasmid

#91111

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: ToLCV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

35S

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPglu-600uDelta13

Plasmid

#13559

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIPglu F16A Delta13

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

F16A in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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XE69 Xenopus Beta-catenin in GFP

Plasmid

#16839

Depositor

Randall Moon

Article

Miller et al J Cell Biol. 1997 Oct 6. 139(1):229-43.

Insert

beta-catenin GFP (ctnnb1.L Frog)

Use

Xenopus expression

Tags

GFP

Available Since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
pI1-MTU-DO-HPH

Plasmid

#160014

Purpose

Integrative vector for K. marxianus, targeting the LAC4 locus. Hygromycin selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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p3E tdTomato

Plasmid

#135210

Purpose

Red fluorescent tag

Depositor

David Tobin

Article

Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

Insert

tdTomato

Use

Synthetic Biology

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB6576

Plasmid

#106134

Purpose

EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_3, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Expression

Yeast

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pEB1-2xEGFP

Plasmid

#37827

Depositor

Torsten Wittmann

Article

Gierke et al Curr Biol. 2012 May 8;22(9):753-62. Epub

Insert

EB1 (MAPRE1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pZ143-pcDNA3-BvCas12b

Plasmid

#122445

Purpose

Expresses BvCas12b in mammalian cells

Depositor

Feng Zhang

Article

Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

Insert

CMV-NLS-BvCas12b-NLS-3xHA

Tags

3XHA

Expression

Mammalian

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pMOD_A3003

Plasmid

#91043

Purpose

Module A, Promoter: CmYLCV, Gene: GFP, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

GFP

Use

Unspecified

Promoter

CmYLCV

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pTRANS_222

Plasmid

#91116

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: ToLCV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

35S

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pHpgRNA13

Plasmid

#160127

Purpose

To express a sgRNA with constant targeting ADE2 by RNA pol II promoter PTEF1

Depositor

Yongjin Zhou

Article

Gao et al iScience. 2021 Feb 9;24(3):102168. doi:

Insert

PTEF1-HH-gRNA-ADE2- HDV-TAMO

Expression

Yeast

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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YIPlac204TKC-E2-Crimson-HDEL

Plasmid

#38771

Depositor

Benjamin Glick

Article

Strack et al Biochemistry. 2009 Sep 8;48(35):8279-81.

Insert

E2-Crimson

Tags

Linker peptide and yeast ER retention signal sequ…

Expression

Yeast

Promoter

TPI1

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pVITRO1-BFP-NLS

Plasmid

#184458

Purpose

Nucleus-targeted EBFP2 expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

EBFP2-3xNLS

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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shNg pA_RC3J1_CAGW

Plasmid

#92155

Purpose

Bicistronic AAV construct encoding GFP control and shRNA targeting Ng

Depositor

Weifeng Xu

Article

Han et al Cell Rep. 2017 Jan 24;18(4):892-904. doi

Insert

shRNA targeting Ng (NRGN Human)

Use

AAV

Expression

Mammalian

Promoter

H1

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pJKR-O-acuR

Plasmid

#62569

Purpose

Acrylate biosensor. Medium copy plasmid that expresses CFP proportional to acrylate concentration. Compatible with all other pJKR plasmids.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

CFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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p33Lb

Plasmid

#158156

Purpose

Expression of crRNA array and LbCas12a using one Pol II promoter. For plant genome editing.

Depositor

Kabin Xie

Article

Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_FliC_IRES_mCherry_PGK_tBFP

Plasmid

#85438

Purpose

Lentiviral vector for Gal4 inducible S. Typhi Flagellin with mCherry reporter and constitutive tBFP expression

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

S. typhi FliC

Use

Lentiviral

Available Since

Nov. 23, 2017

Availability

Academic Institutions and Nonprofits only
Paxillin

Plasmid

#186142

Purpose

For expresseion of recombinant paxillin in bacteria

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PXN (PXN Human)

Tags

GST

Expression

Bacterial

Mutation

Paxillin C1997A (silent mutation for cloning)

Promoter

Lac

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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p3E p2a-tomato

Plasmid

#135211

Purpose

Red fluorescent tag

Depositor

David Tobin

Article

Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

Insert

tdTomato

Use

Synthetic Biology

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pTRANS_150

Plasmid

#91205

Purpose

Transformation, Type: non-T-DNA, Plant Selection: PvUbi2:hpt II, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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