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pJK-caCas9-NatMX-Neut5L-Ade2 drive

Plasmid

#89577

Purpose

caCas9 integrating vector into the Neut5L locus with gene drive for targeting Ade2 locus

Depositor

George Church

Article

Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

Insert

Ade2 gene drive

Use

Synthetic Biology

Expression

Yeast

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-Cbir-Ago2_Myc-Nbir-GFP

Plasmid

#90015

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-GFP

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

CBirA*-Ago2
NBirA*-GFP

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pEasyG2_hph

Plasmid

#184915

Purpose

Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_hph recombines in vivo with a PCR product from pEasyG2_mic.

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pDNR-SpCas9-Cdt1

Plasmid

#80426

Purpose

Used for in vitro transcription of SpCas9-Cdt1 mRNA. Cas9 is fused to Cdt1 peptide and is degraded during S/G2 phases of the cell-cycle.

Depositor

Sara Howden, Melissa Little

Article

Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

Insert

SpCas9-Cdt1

Use

CRISPR

Tags

Cdt1

Mutation

codon optimized for human

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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FKBP-epsin(144-575)-GFP

Plasmid

#100733

Purpose

To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

epn1 (Epn1 Mouse)

Tags

FKBP and GFP

Expression

Mammalian

Mutation

clathrin binding middle part aa 144-575

Promoter

CMV

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBacM13-8His-ZZ-LTLT-Hook3-SNAPf

Plasmid

#130976

Purpose

Expression of 8His-ZZ-LTLT tagged Hook3-SNAPf in insect cells for protein purification.

Depositor

Anne Straube

Article

Siddiqui et al Nat Commun. 2019 Jun 19;10(1):2693. doi:

Insert

Hook3-SNAPf (HOOK3 Human)

Tags

8His-ZZ-LTLT and SNAPf

Expression

Insect

Promoter

Polyhedrin

Available Since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pWPI-UPP1-3xFLAG

Plasmid

#201644

Purpose

Over-expression of 3xFLAG-tagged human UPP1

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

UPP1 (UPP1 Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pET22b-T5-sfGFP*- MmH-MH4R

Plasmid

#196409

Purpose

Express sfGFP with TAG and enzymes for biosynthesizing DOPA

Depositor

Han Xiao

Article

Chen et al J Mol Biol. 2021 Dec 20:167412. doi: 10.

Inserts

sfGFP with TAG at 151 position
Tyrosine Hydroxylase (Th Mouse)
dihydropterine reductase from human (QDPR Human)
pterin-4a-carbinolamine dehydratase from human

Expression

Bacterial

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/MPL-V5

Plasmid

#204531

Purpose

Mammalian expression of human MPL-V5

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

MPL-V5 (MPL Human)

Expression

Mammalian

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-mCD24-T2A-GFP

Plasmid

#205448

Purpose

lentiviral plasmid for expression of mouse CD24

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

mCD24 (Cd24a Mouse)

Expression

Mammalian

Promoter

UBC

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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nuc-GafD-mTurboID-HA

Plasmid

#184641

Purpose

Expresses 3xHA-tagged GlycoID construct: GafD_short-miniTurboID in mammalian nucleus for O-GlcNAc interactome tagging

Depositor

Charlie Fehl

Article

Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021

Insert

nuc-GlycoID-HA

Tags

3xHA tag and Nuclear localization sequence

Expression

Mammalian

Mutation

GafD -> expressed 22-178 ; BirA* -> express…

Promoter

CMV

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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LUC05 (L/Cys/P)

Plasmid

#112146

Purpose

Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

Depositor

Paul Copeland

Article

Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.

Insert

Luciferase coding region with wild type Cysteine 258 and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Photinus pyralis, Rat)

Expression

Mammalian

Mutation

none

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pSLIK 3xFLAG-KPNA2 zeo

Plasmid

#192330

Purpose

Lentiviral expression vector for an inducible 3xFLAG-KPNA2

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

KPNA2 (KPNA2 Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pSS1 (hLPL-FLAG)

Plasmid

#102447

Purpose

Expresses FLAG-tagged human LPL in mammalian cells

Depositor

Brandon Davies

Article

Chi et al J Biol Chem. 2015 May 8;290(19):11865-77

Insert

Lipoprotein lipase (LPL Human)

Tags

6x His and FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 21, 2018

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast CHKA

Plasmid

#218540

Purpose

human CHKA cDNA

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

CHKA (CHKA Human)

Use

Retroviral

Expression

Mammalian

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pKT2/CAGXSP/PalmReNL

Plasmid

#182970

Purpose

Stably express PalmReNL in mammalian cells via the sleeping beauty transposon system.

Depositor

Masamitsu Kanada

Article

Perez et al Adv Genet (Hoboken). 2022 Jan 19;3(1):21

Insert

PalmReNL

Expression

Mammalian

Mutation

N/A

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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MYH11-gRNA1

Plasmid

#132587

Purpose

gRNA used for knockin NanoLuc and tdTomado (separated by 2A) into MYH11 allele (MYH11-NanoLuc-2A-tdTomato vector) )

Depositor

James Thomson

Article

Zhang et al Stem Cell Reports. 2019 Jun 11;12(6):126

Insert

MYH11-gRNA1

Expression

Bacterial

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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PB-mCar-EGFP-WPRE-BGH-pA-UBC-Bla

Plasmid

#174620

Purpose

PiggyBac plasmid expressing EGFP under the control of a cone arrestin promoter

Depositor

Volker Busskamp

Article

Gasparini et al J Clin Invest. 2022 Apr 28. pii: 154619.

Insert

enhanced green fluorescent protein

Use

Piggybac

Expression

Mammalian

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV2_hSyn_NES-Caprola_on-mEGFP_WRPE-SV40

Plasmid

#194691

Purpose

hSyn1 driven expression of the consitutively active calcium recorder positive control Caprola_on fused to mEGFP for neuronal expression through AAV transduction

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_on-mEGFP

Use

AAV

Tags

mEGFP

Promoter

hSyn1

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223-MCU-D261A

Plasmid

#31729

Depositor

Vamsi Mootha

Article

Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

Insert

MCU (MCU Human)

Use

Pdonr gateway

Tags

None

Mutation

Changed D261 to Alanine

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pT2/sh-Col1a1/GFP4_Seq1.2

Plasmid

#201403

Purpose

Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

Insert

COL1A1

Expression

Mammalian

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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PGK EGFP-PPM1H-WT

Plasmid

#200084

Purpose

Mammalian expression of wild-type PPM1H

Depositor

Erika Holzbaur

Article

Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

Insert

PPM1H (PPM1H Human)

Tags

EGFP

Expression

Mammalian

Promoter

PGK

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACt-mCherry-NLS

Plasmid

#138214

Purpose

Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; nuclear targeted.

Depositor

Jin Zhang

Article

Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

Insert

sACt-mCherry-NLS (Adcy10 Rat)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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SBP-GFP-Tac-TC

Plasmid

#162506

Purpose

Expresses GFP tagged partial length Tac (TMD and cytosolic tail) in RUSH vector in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

Insert

GFP and SBP tagged partial length Tac (TMD and cytosolic tail) (IL2RA Human)

Tags

GFP and SBP

Expression

Mammalian

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-hCD46-T2A-GFP

Plasmid

#205457

Purpose

lentiviral plasmid for expression of human CD46

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

hCD46 (CD46 Human)

Expression

Mammalian

Promoter

UBC

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAcBac1-Pyl-TAG

Plasmid

#228784

Purpose

Pyl aaRS/tRNA system for mammalian TAG suppression

Depositor

Abhishek Chatterjee

Article

Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

Inserts

PylRS
PytR

Use

Baculoviral

Expression

Mammalian

Mutation

TAG suppressor

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223-MCU-E264A

Plasmid

#31730

Depositor

Vamsi Mootha

Article

Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

Insert

MCU (MCU Human)

Use

Pdonr gateway

Tags

None

Mutation

Changed E264 to Alanine

Promoter

None

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pB80-LAMP1-mCherry-iLID

Plasmid

#174625

Purpose

Optogenetic coupling to lysosomes (LAMP1) via iLID

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

LAMP1-mCherry-iLID (LAMP1 Synthetic, Human)

Tags

mCherry-iLID

Expression

Mammalian

Mutation

mCherry: Met1Del

Promoter

Chicken beta-actin

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMRX-IP/Venus-rLC3

Plasmid

#58740

Purpose

Expresses Venus-rLC3 in mammalian cells

Depositor

Noboru Mizushima

Article

Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Retroviral

Tags

Venus

Available Since

Dec. 8, 2014

Availability

Academic Institutions and Nonprofits only
pLentiCRISPRv1_SDHB

Plasmid

#177981

Purpose

lentiviral vector expressing Cas9 and a sgRNA targeting SDHB

Depositor

David Sabatini, Jessica Spinelli

Article

Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

Insert

sgRNA targeting SDHB (SDHB Human)

Use

Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

U6

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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