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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,022 results for: ENA;

Showing: 5321 - 5340 of 11022 results

  2. pPPC011

    Plasmid
    #171143
    Purpose
    Expression of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) on pRK2-KmR plasmid
    Depositor
    Jesse Zalatan
    Article
    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(
    Insert
    Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    SoxS has R93A and S101A mutations
    Promoter
    Sp.pCas9, BBa_J23107
    Available Since
    Oct. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    171143-plasmid-map-sequence-id-345216

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  3. AA145

    Plasmid
    #215937
    Purpose
    Fragmid fragment: (guide cassette) guide expression; Chen F+E trRNA (preferred)
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v4 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215937-plasmid-map-sequence-id-431395

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  5. pBF_HsPepT1

    Plasmid
    #73943
    Purpose
    Xenopus oocyte expression vector containing the cDNA of human PepT1 with a c terminal flag tag
    Depositor
    Simon Newstead
    Article
    Beale et al Structure. 2015 Oct 6;23(10):1889-99. do
    Insert
    PepT1 (SLC15A1 Human)
    Use
    Make rna for xenopus oocyte injection
    Tags
    Flag
    Expression
    Mutation
    Promoter
    beta globin
    Available Since
    April 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  6. pBF_HsPepT2

    Plasmid
    #73944
    Purpose
    Xenopus oocyte expression vector containing the cDNA of human PepT2 with a c terminal flag tag
    Depositor
    Simon Newstead
    Article
    Beale et al Structure. 2015 Oct 6;23(10):1889-99. do
    Insert
    PepT2 (SLC15A2 Human)
    Use
    Make rna for xenopus oocyte injection
    Tags
    Flag
    Expression
    Mutation
    Promoter
    beta globin
    Available Since
    April 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  7. LSB-hsa-miR-181a-5p

    Plasmid
    #103276
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-181a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-181a-5p target
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103276-plasmid-map-sequence-id-195601

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  14. LSB-hsa-miR-941

    Plasmid
    #103757
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-941 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-941 target
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103757-plasmid-map-sequence-id-196562

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  15. pPKm-248

    Plasmid
    #90504
    Purpose
    Expresses HO1, PCYA, FD and FNR, required for PCB synthesis. pSIN - EF-1alpha - MTS - tPCYA - IRES - MTS - tHO1 - P2A - MTS - tFD - P2A - MTS - tFNR
    Depositor
    Todd Coleman
    Article
    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717
    Insert
    mito-tHO1, mito-tPCYA, mito-tFD, mito-tFNR
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    April 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    90504-plasmid-map-sequence-id-204704

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  18. pCas9-beta-mmr

    Plasmid
    #169241
    Purpose
    Cas9, beta, and mutL mutant expression plasmid for introducing chromosomal point mutations
    Depositor
    Brian Pfleger
    Article
    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:
    Inserts
    cas9
    beta
    mutL-E36K
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Changed glutamic acid 36 to lysine
    Promoter
    Available Since
    June 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    169241-plasmid-map-sequence-id-333575

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  20. pAAV-CAG-DIO-(STChRger2-TS-EYFP)

    Plasmid
    #129394
    Purpose
    Soma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Uses the CAG promoter and double floxed.
    Depositor
    Viviana Gradinaru
    Article
    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s
    Insert
    soma targeted ChRger2
    Use
    AAV and Cre/Lox
    Tags
    Kv2.1-TS-EYFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available Since
    Oct. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    129394-plasmid-map-sequence-id-259728

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Showing: 5321 - 5340 of 11022 results