We narrowed to 5,840 results for: pCas

1. pX330A-nBRD4/PITCh

   Plasmid

   #91794

   Purpose

   PITCh sgRNA, N-terminal BRD4 sgRNA, and Cas9 expressing plasmid for use with the dTAG knock-in system and BRD4

   Depositor

   Behnam Nabet, James Bradner

   Article

   Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

   Insert

   SpCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   chicken β-actin promoter

   Available Since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRN3P_T3_ABE8e_IVT

   Plasmid

   #201676

   Purpose

   Plasmid to be used as DNA template for in vitro RNA transcription of the ABE8e base editor (A to G) by T3 RNA polymerase. The plasmid contains optimised 5'UTR and 3'UTR to improve protein expression.

   Depositor

   Timo Otonkoski

   Article

   Jalil et al Am J Hum Genet. 2024 Apr 4;111(4):714-72

   Insert

   ecTadA(8e)-nSpCas9
   

   Use

   CRISPR; Vector for in-vitro transcription

   Promoter

   T3 promoter

   Available Since

   April 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. SIN40C.SFFV.dCas9-KRAB.P2A.mNeon

   Plasmid

   #170482

   Purpose

   Lentiviral dSpCas9-KRAB (CRISPRi) delivery, mNeon coexpression

   Depositor

   Dirk Heckl

   Article

   Ng et al iScience. 2023 Sep 7;26(10):107844. doi:

   Inserts

   dCas9-KRAB
   SFFV
   P2A-mNeonGreen
   

   Use

   Lentiviral

   Tags

   HA

   Available Since

   Aug. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDicAID_nCas9-PmCDA_NptII_Della

   Plasmid

   #91694

   Purpose

   Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1 with sgRNA targeting SlDella

   Depositor

   Akihiko Kondo

   Article

   Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   SpCas9
   

   Tags

   PmCDA1

   Expression

   Plant

   Mutation

   D10A for nickase Cas9

   Promoter

   PcUbi

   Available Since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. SPDK3876(TRV-RNA2-pPEBV-MCS)

   Plasmid

   #149275

   Purpose

   Modified Tobacco rattle virus RNA2 vector that could be used for expression of proteins or RNAs

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

   Insert

   Modified TRV RNA2 with PEBV promoter
   

   Use

   T-dna vector

   Mutation

   T2318C (DNA) in PEBV coat protein sequence

   Available Since

   June 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Hygro-Cas9 donor

   Plasmid

   #86883

   Purpose

   Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus with hygromycin selection

   Depositor

   Danwei Huangfu

   Article

   Shi et al Cell Stem Cell. 2017 Feb 8. pii: S1934-5

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3xFlag, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Available Since

   March 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. SOX17-3'gRNA

   Plasmid

   #210465

   Purpose

   gRNA targeting SOX17 3' terminal for CRISPR-Cas9-mediated knock-in

   Depositor

   Kai Wang

   Article

   Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

   Insert

   SOX17 (SOX17 Human)
   

   Use

   CRISPR

   Available Since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330-PITCh-GOLGA2

   Plasmid

   #207791

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of GOLGA2 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of GOLGA2 (GOLGA2 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-CANX

   Plasmid

   #227279

   Purpose

   Expresses SpCas9 and a sgRNA targeting the C-terminus of CANX for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of CANX (CANX Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPBT/EFS-Cas9-PuroTk

    Plasmid

    #170542

    Purpose

    Expresses spCas9 with a P2A junction to the puromycin resistance gene directly fused to the thymidine kinase

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

    Insert

    PuroTK
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330-PITCh-TOMM20

    Plasmid

    #207789

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of TOMM20 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of TOMM20 (TOMM20 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. PRDM9(KRAB-SSXRD-PR/SET-ZF1-dC)-Cas9

    Plasmid

    #184464

    Purpose

    Plasmid encoding PRDM9-Cas9 fusion under CMV promoter

    Depositor

    Jennifer Doudna

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

    Insert

    PRDM9(KRAB-SSXRD-PR/SET-ZF1-dC)-Cas9 (PRDM9 Human)
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-U6-gRNA-UbC-DsRed-P2A-Bsr

    Plasmid

    #83919

    Purpose

    Lentiviral SpCas9-gRNA expression vector with DsRed-Express2-P2A-BlastR

    Depositor

    Charles Gersbach

    Article

    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

    Inserts

    DsRed-Express2
    Bsr
    

    Use

    CRISPR and Lentiviral

    Tags

    P2A

    Promoter

    hUbC

    Available Since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. NKP38 GFP (1009)

    Plasmid

    #62021

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Nucleus. 2012 Nov-Dec;3(6):552-64. doi:

    Insert

    AYTL1 (LPCAT2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

15. iCAP-BI-28-KanR

    Plasmid

    #203532

    Purpose

    RepCap for AAV production

    Depositor

    Ben Deverman

    Article

    Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

    Insert

    AAV-BI28 Cap
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330-PITCh-VIM

    Plasmid

    #227301

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of VIM for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of VIM (VIM Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. AXBEv2

    Plasmid

    #204606

    Purpose

    Mammalian expression of evolved adenine transversion base editor AXBEv2

    Depositor

    Dali Li

    Article

    Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

    Insert

    bpNLS-TadA-8e-nSpCas9-mAAG (R165E/Y179F)-bpNLS-P2A-GFP
    

    Use

    CRISPR

    Tags

    6*His

    Expression

    Mammalian

    Mutation

    mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)

    Available Since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRubiG-T2A-Cas9

    Plasmid

    #75348

    Purpose

    Ubiquitin promoter expresses GFP and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.

    Depositor

    Bryan Luikart

    Article

    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

    Insert

    Cas9
    

    Use

    CRISPR and Retroviral

    Tags

    GFP (via t2a)

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. VIPR2-Tango

    Plasmid

    #66521

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    VIPR2 (VIPR2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pX330-PITCh-MAPRE1

    Plasmid

    #207793

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of MAPRE1 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of MAPRE1 (MAPRE1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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