We narrowed to 9,180 results for: mel

1. pCMV-BE4-PpAPOBEC1 H122A

   Plasmid

   #138338

   Purpose

   Mammalian expression plasmid for BE4-PpABOBEC1H122A

   Depositor

   Nicole Gaudelli

   Article

   Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

   Insert

   BE4-PpAPOBEC1 H122A
   

   Use

   Synthetic Biology

   Tags

   BP-NLS

   Mutation

   Change of His122 to Ala.

   Promoter

   CMV

   Available Since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMM0638

   Plasmid

   #129005

   Purpose

   Integration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2

   Depositor

   Megan McClean

   Article

   An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

   Insert

   5' URA3 -pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-URA3 URA3 3'- KanR-ColE1
   

   Expression

   Yeast

   Available Since

   Oct. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. roGFP_iE_C147S_pQE30

   Plasmid

   #48631

   Purpose

   Bacterial expression vector encoding for the 6XHis tagged insensitive variant of fluorescent redox probe roGFPiE with Cysteine 147 to Serine mutation (roGFP C147S, E147b, C204)

   Depositor

   David Ron

   Article

   Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

   Insert

   roGFP1
   

   Tags

   HisTag

   Expression

   Bacterial

   Mutation

   insertion E147B, C147S

   Available Since

   Oct. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGK:HygroR-CMV:mTagBFP2-A_UTR

   Plasmid

   #68479

   Purpose

   Lentivirus expressing mTagBFP2 with UTR sensor cassette and hygromycin resistance

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Inserts

   Hygromycin Resistance
   mTagBFP2-A_UTR
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   shRNA sensor cassette "A" added to 3&#x…

   Promoter

   CMV and pGK

   Available Since

   Nov. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSws1-nfsB-CFP

   Plasmid

   #86375

   Purpose

   for zebrafish transgenesis, expression of nfsB-CFP, also expresses EGFP

   Depositor

   Rachel Wong

   Article

   Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

   Insert

   nitroreductase (nfsB bacteria)
   

   Use

   Zebrafish

   Tags

   ECFP

   Available Since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. F-CDK8L

   Plasmid

   #24762

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Conaway et al FEBS Lett. 2005 Feb 7. 579(4):904-8.

   Insert

   F-CDK8L (CDK19 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   May 14, 2010

   Availability

   Academic Institutions and Nonprofits only

7. PB-CT3G-ERP2-MG-IRES2-mNeonGreen

   Plasmid

   #175506

   Purpose

   All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (hEF1a-driven rtTA and puromycin resistance)

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   TRE3G-mCMV

   Available Since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCOLA-Gent-yibDp-Erv1p

   Plasmid

   #202483

   Purpose

   Expresses sulfydryl oxidase (Erv1p) after phosphate depletion

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   sulfydryl oxidase (Erv1p) (ERV1 Budding Yeast)
   

   Expression

   Bacterial

   Promoter

   yibDp

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUBC_HA_PP7-mCherry

   Plasmid

   #107605

   Purpose

   Transient expression of non-aggregating delta-FG mutant, fused to mCherry in mammalian cells, under a hUBC promoter, HA tag

   Depositor

   John Rinn

   Article

   Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10

   Insert

   PP7-mCherry
   

   Use

   Synthetic Biology

   Tags

   mCherry

   Mutation

   PP7: [delta]67_74 (non-aggregating)

   Promoter

   hUBC

   Available Since

   April 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFUGW-ZipACR-EYFP

    Plasmid

    #88844

    Purpose

    Fast anion channelrhodopsin (current half-decay time 2-4 ms depending on voltage) for time-resolved inhibition of neuronal spiking

    Depositor

    John Spudich

    Article

    Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

    Insert

    ZipACR-NotI-EYFP
    

    Use

    Lentiviral

    Tags

    EYFP

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti_CRISPRi_sgNeg1

    Plasmid

    #166998

    Purpose

    Lentiviral expression of negative control sgRNA for CRISPRi knockdown

    Depositor

    Gian-Paolo Dotto

    Article

    Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

    Insert

    Negative control sgRNA 1
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. GST-MAGE-2-429

    Plasmid

    #89129

    Purpose

    bacterial expression of full-length human MAGE-A11 with N-terminal GST tag for rapid isolation, in pGEX-4T-1 EcoRI and XhoI sites

    Depositor

    Elizabeth Wilson

    Article

    Askew et al Mol Cell Endocrinol. 2017 Mar 5;443:42-5

    Insert

    MAGE-A11 (MAGEA11 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

13. FLAG-GAS41

    Plasmid

    #15428

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

    Insert

    GAS41 (Yeats4 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pCMV-BE4-RrA3F F130L

    Plasmid

    #138341

    Purpose

    Mammalian expression plasmid for BE4-RrA3F F130L

    Depositor

    Nicole Gaudelli

    Article

    Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

    Insert

    BE4-RrA3F F130L
    

    Use

    Synthetic Biology

    Tags

    BP-NLS

    Promoter

    CMV

    Available Since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. GFP-GW182delta1

    Plasmid

    #11592

    Depositor

    Edward Chan

    Article

    Jakymiw et al Nat Cell Biol. 2005 Dec . 7(12):1267-74.

    Insert

    GW182 (TNRC6A Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains only aa1-498; SEE DEPOSITOR NOTES BELOW

    Available Since

    June 30, 2006

    Availability

    Academic Institutions and Nonprofits only

16. SNF-5 pXOOM

    Plasmid

    #48697

    Purpose

    Expressed SNF-5 in Xenopus oocytes or mammalian cells

    Depositor

    Dmitri Boudko

    Article

    Metzler et al J Exp Biol. 2013 Aug 1;216(Pt 15):2843-5

    Insert

    snf-5 (snf-5 Nematode)
    

    Use

    Xenopus oocyte expression

    Expression

    Mammalian

    Mutation

    K42E (when compared to NP_496735.1)

    Promoter

    T7

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX459-sg-mGreenLantern/EGFP

    Plasmid

    #179913

    Purpose

    All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting mGreenLantern and EGFP fluorescent proteins.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    mGreenLantern/EGFP sgRNA
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGK:TRL-EFS:iRFP670

    Plasmid

    #68475

    Purpose

    Lentiviral plasmid expressing tTR-KRAB-rtTA3-Luc cassette and iRFP670

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Inserts

    tTR-KRAB-2A-rtTA3-2A-Luciferase
    iRFP670
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Promoter

    EFS and pGK

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a(TIGIT)

    Plasmid

    #177902

    Purpose

    Bacterial expression of his10-tagged human TIGIT Intracellular Domain

    Depositor

    Ron Vale

    Article

    Hui et al Science. 2017 Mar 31;355(6332):1428-1433

    Insert

    TIGIT (aa 163-244 only) (TIGIT Human)
    

    Tags

    10xHis-Thrombin cut site-T7 tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. p168

    Plasmid

    #62618

    Purpose

    ePathBrick pETM6 vector encoding CsF3H, FaDFR, and DuLAR

    Depositor

    Mattheos Koffas

    Article

    Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1

    Inserts

    flavaone 3B-hydroxylase
    dihydroflavonol 4-reductase
    leucoanthocyanidin reductase
    

    Use

    Synthetic Biology

    Tags

    GBD, PDZ, and SH3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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