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169,779 results

Showing: 521 - 540 of 169779 results

  2. HCN3/pCDNA3.1

    Plasmid
    #197293
    Purpose
    Plasmid expressing an antigen targeting the HCN3 ion channel subunit
    Depositor
    Dane Chetkovich
    Insert
    HCN3 (Hcn3 Mouse)
    Expression
    Mammalian
    Available Since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    197293-plasmid-map-sequence-id-388075

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  7. pLNCX1 HA AKT2

    Plasmid
    #60129
    Purpose
    expresses HA tagged AKT2
    Depositor
    Morris Birnbaum
    Insert
    protein kinase B (AKT2 Human)
    Use
    Retroviral
    Tags
    HA
    Promoter
    CMV
    Available Since
    Oct. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    60129-plasmid-map-sequence-id-96079

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  10. pHA AKT2 T309A/S474A

    Plasmid
    #60128
    Purpose
    expresses a mutant AKT2
    Depositor
    Morris Birnbaum
    Insert
    protein kinase B (AKT2 Human)
    Use
    Retroviral
    Tags
    HA
    Mutation
    Serine 474 and Threonine 309 changed to alanines
    Promoter
    CMV
    Available Since
    Oct. 27, 2014
    Availability
    Academic Institutions and Nonprofits only

  14. pPB_Cdc42-TomKat-sensor-CAAX

    Plasmid
    #172473
    Purpose
    Piggybac vector for mammalian expression of TomKat (tdTomato-tdKatushka) FRET sensor for Cdc42 activity anchored to the membrane with the polybasic and CAAX motif from Kras.
    Depositor
    Sean R Collins
    Article
    Bell et al Nat Commun. 2021 Nov 16;12(1):6148. doi:
    Insert
    tdKatushka2-PBD(Pak1)-EVlinker-Cdc42-tdTomato-CAAX
    Use
    Piggybac transposon
    Tags
    polybasic and CAAX motif from Kras, tdKatushka2, …
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Sept. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    172473-plasmid-map-sequence-id-342817

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  15. AAVS1_puro_CAG_HexaPro

    Plasmid
    #164077
    Purpose
    Targeted integration of SARS-CoV-2 spike HexaPro variant into the AAVS1 genomic safe harbor locus
    Depositor
    Yannick Doyon
    Article
    Rivest et al protocols.io 2021
    Insert
    SARS-CoV-2 S HexaPro (S Severe acute respiratory syndrome coronavirus 2)
    Use
    CRISPR
    Tags
    2X Strep-Tag II, 8X His tag, and HRV 3C cleavage …
    Expression
    Mammalian
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Promoter
    CAG
    Available Since
    Jan. 4, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    164077-plasmid-map-sequence-id-320680

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  16. FLAG-hLRP6-opt(20-1613)-9xHis_MESD_pFastBacDual

    Plasmid
    #216377
    Purpose
    Baculovirus transfer vector to co-express FLAG-LRP6-His (human sequence, codon-optimized for insect cell expression) and MESD chaperone (human sequence)
    Depositor
    William Weis
    Insert
    LDL receptor related protein 6 (LRP6 Human)
    Use
    Baculovirus
    Tags
    9xHis and HA signal sequence-FLAG-3C cleavage sit…
    Expression
    Insect
    Promoter
    Polyhedrin (LRP6)/p10 (MESD)
    Available Since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    216377-plasmid-map-sequence-id-433174

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  18. pD649-HAsp-CD19-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156714
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    CD19 (CD19 Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156714-plasmid-map-sequence-id-407030

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Showing: 521 - 540 of 169779 results