We narrowed to 17,342 results for: She

1. pCMV-ALFA-MCP

   Plasmid

   #207628

   Purpose

   Expresses ALFA-tagged MCP fusion protein in mammalian cells (with a CMV promoter)

   Depositor

   Jay Shendure

   Article

   Choi et al Elife. 2025 Feb 12;13:RP98110. doi: 10.7

   Insert

   ALFA-MCP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   pCMV

   Available since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1-Zeo-ssIFNA2

   Plasmid

   #183469

   Purpose

   Expression of the Salmo salar interferon ifna2

   Depositor

   Bertrand Collet

   Article

   Collins et al Fish Shellfish Immunol. 2014 Oct;40(2):6

   Insert

   Salmo salar interferon alpha 2 precursor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1-Zeo-ssIFNG

   Plasmid

   #183470

   Purpose

   Expression of the Salmo salar gamma interferon

   Depositor

   Bertrand Collet

   Article

   Collins et al Fish Shellfish Immunol. 2014 Oct;40(2):6

   Insert

   Salmo salar interferon gamma precursor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. relAE335Q_NS1

   Plasmid

   #100917

   Purpose

   expresses E.coli relAE335Q from the NS1 site in the S. elongatus PCC7942 genome

   Depositor

   Erin O'Shea

   Article

   Puszynska et al Cell Rep. 2017 Dec 12;21(11):3155-3165.

   Insert

   RelA fragment from E.coli with point mutation E335Q
   

   Use

   Cyanobacteria cloning vector

   Tags

   FLAG

   Expression

   Mutation

   E 335 converted to Q

   Promoter

   Available since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. KFP08

   Plasmid

   #119798

   Purpose

   SigF2 epitope tagged construct for integration at neutral site 2.2

   Depositor

   Erin O'Shea

   Article

   Fleming et al Cell Rep. 2018 Dec 11;25(11):2937-2945.e

   Insert

   Synpcc7942_1784
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCR3.1-OPNc PSM1

   Plasmid

   #106328

   Purpose

   Mammalian expression of mutant osteopontin

   Depositor

   Georg Weber

   Article

   Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

   Insert

   OPNc (SPP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   OPNc phosphorylation site mutant SGSSEEKQNAVSSEET…

   Promoter

   Available since

   June 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCR3.1-OPNc PSM3

   Plasmid

   #106327

   Purpose

   Mammalian expression of mutant osteopontin

   Depositor

   Georg Weber

   Article

   Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

   Insert

   OPNc (SPP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   OPNc phosphorylation site mutant SGSSEEKQNAVSSEET…

   Promoter

   Available since

   June 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCR3.1-OPNc PSM2

   Plasmid

   #106326

   Purpose

   Mammalian expression of mutant osteopontin

   Depositor

   Georg Weber

   Article

   Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

   Insert

   OPNc (SPP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   OPNc phosphorylation site mutant SGSSEEKQNAVSSEET…

   Promoter

   Available since

   June 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCR3.1-OPNc M1

   Plasmid

   #106321

   Purpose

   Mammalian expression of mutant osteopontin

   Depositor

   Georg Weber

   Article

   Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

   Insert

   OPNc (SPP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   OPNc mutated splice site EEKQ-->AAAA

   Promoter

   Available since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCR3.1-OPNc PSM4

    Plasmid

    #106329

    Purpose

    Mammalian expression of mutant osteopontin

    Depositor

    Georg Weber

    Article

    Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

    Insert

    OPNc (SPP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    OPNc phosphorylation site mutant SGSSEEKQNAVSSEET…

    Promoter

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCR3.1-OPNc M5

    Plasmid

    #106325

    Purpose

    Mammalian expression of mutant osteopontin

    Depositor

    Georg Weber

    Article

    Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

    Insert

    OPNc (SPP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    OPNc splice site insertion mutant EEKQNA-->EEK…

    Promoter

    Available since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCR3.1-OPNc M4

    Plasmid

    #106324

    Purpose

    Mammalian expression of mutant osteopontin

    Depositor

    Georg Weber

    Article

    Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

    Insert

    OPNc (SPP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    OPNc splice site deletion mutant EEKQNA-->EEKNA

    Promoter

    Available since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCR3.1-OPNc M3

    Plasmid

    #106323

    Purpose

    Mammalian expression of mutant osteopontin

    Depositor

    Georg Weber

    Article

    Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

    Insert

    OPNc (SPP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    OPNc mutated splice site EEKQNAV-->AAAAAAA

    Promoter

    Available since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRec-GalS

    Plasmid

    #104573

    Purpose

    Expresses Cas1, Cas2 under PLTetO-1 promoter, and TetR and LacI-GalS chimera constitutively.

    Depositor

    Harris Wang

    Article

    Sheth et al Science. 2017 Dec 15;358(6369):1457-1461

    Insert

    PTet-cas12, tetR, lacI-galS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRec-TreR

    Plasmid

    #104574

    Purpose

    Expresses Cas1, Cas2 under PLTetO-1 promoter, and TetR and LacI-TreR chimera constitutively.

    Depositor

    Harris Wang

    Article

    Sheth et al Science. 2017 Dec 15;358(6369):1457-1461

    Insert

    PTet-cas12, tetR, lacI-treR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. rpaA_NS1

    Plasmid

    #101829

    Purpose

    drives expression of rpaA from the NS1 site in the S. elongatus PCC7942 genome

    Depositor

    Erin O'Shea

    Article

    Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

    Insert

    RpaA with its native promoter
    

    Use

    Cyanobacteria cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. P0050::rpaA

    Plasmid

    #101830

    Purpose

    drives expression of rpaA from an rpaA-independent promoter

    Depositor

    Erin O'Shea

    Article

    Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

    Insert

    RpaA with p0050 promoter (+ gentamicin resistance casette)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Ptrc::galP_NS1

    Plasmid

    #101843

    Purpose

    drives expression of galP transporter from NS1 locus

    Depositor

    Erin O'Shea

    Article

    Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

    Insert

    Ptrc promoter driving GalP transporter expression
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJRL2 GAL1(TATA-C2)pr VYFP (EB#1577)

    Plasmid

    #14851

    Depositor

    Erin O'Shea

    Article

    Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

    Insert

    GAL1 promoter TATA-C2 (GAL1 Budding Yeast)
    

    Use

    Tags

    VYFP

    Expression

    Yeast

    Mutation

    TATA-C2. TATA box mutated from TATATAAG to TCTATA…

    Promoter

    Available since

    May 25, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pJRL2 GAL1(TATA-C2)pr ECFP (EB#1578)

    Plasmid

    #14852

    Depositor

    Erin O'Shea

    Article

    Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

    Insert

    GAL1 promoter TATA-C2 (GAL1 Budding Yeast)
    

    Use

    Tags

    ECFP

    Expression

    Yeast

    Mutation

    TATA-C2. TATA box mutated from TATATAAG to TCTATA…

    Promoter

    Available since

    May 25, 2007

    Availability

    Academic Institutions and Nonprofits only

21. pBAD-KIRIN1-GR

    Plasmid

    #107417

    Purpose

    Bacterial Expression plasmid for potassium ion raitometric indicator KIRIN1-GR

    Depositor

    Robert Campbell

    Article

    Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

    Insert

    KIRIN1-GR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAM5409

    Plasmid

    #132662

    Purpose

    RSF1010-based broad host-range plasmid with RK2 bom site expressing YFP and Sp/Sm resistance

    Depositor

    Susan Golden

    Article

    Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

    Inserts

    aadA
    YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PaadA and PconII

    Available since

    Nov. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAM5505

    Plasmid

    #132664

    Purpose

    pRL623-based helper plasmid for conjugations, with RSF1010 mobA gene

    Depositor

    Susan Golden

    Article

    Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

    Insert

    MobA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    please see depositor comments below

    Promoter

    p1,p3

    Available since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Beta-arrestin2 GFP WT

    Plasmid

    #35411

    Depositor

    Robert Lefkowitz

    Article

    Shenoy et al J Biol Chem. 2005 Apr 15;280(15):15315-2

    Insert

    βarrestin2 (Arrb2 Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Lenti-Cas9-gRNA-GFP

    Plasmid

    #124770

    Purpose

    3rd generation lentiviral plasmid encoding Cas9, GFP, and a gRNA backbone

    Depositor

    Jason Sheltzer

    Article

    Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBad-HisB-RRvT

    Plasmid

    #87364

    Purpose

    Expresses RRvT, a tandem heterodimer red fluorescent protein

    Depositor

    Robert Campbell

    Article

    Wiens et al Chembiochem. 2016 Oct 26. doi: 10.1002/c

    Insert

    RRvT
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBad (Arabinose)

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET28a-KIRIN1

    Plasmid

    #107414

    Purpose

    Bacterial Expression plasmid for potassium ion raitometric indicator KIRIN1

    Depositor

    Robert Campbell

    Article

    Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

    Insert

    KIRIN1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBAD-LSSmCherry1

    Plasmid

    #89986

    Purpose

    Long stokes-shift variant of red fluorescent protein mCherry

    Depositor

    Robert Campbell

    Article

    Shen et al PLoS One. 2017 Feb 27;12(2):e0171257. do

    Insert

    LSSmCherry1
    

    Use

    Tags

    6xHis-T7-Xpress

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBbAW4k-loxP-TT-loxP-mRFP1

    Plasmid

    #134405

    Purpose

    Fluorescent reporter for Cre recombinase activity. Cre removes a transcription terminator, leading to RFP production.

    Depositor

    Mary Dunlop

    Article

    Sheets et al ACS Synth Biol. 2020 Feb 21;9(2):227-235

    Insert

    W4-loxP-TT-loxP-mRFP1
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    W4 (modified T7A1)

    Available since

    Jan. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. NLS-Halo

    Plasmid

    #200197

    Purpose

    Expression for SV40 NLS

    Depositor

    Sheila Teves

    Article

    Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

    Insert

    3X SV40 NLS-Halo
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    None

    Promoter

    EF_1alpha

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Phat2-dr-cPla2-C2-EGFP-6H

    Plasmid

    #187001

    Purpose

    Protein Expression

    Depositor

    Philipp Niethammer

    Article

    Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

    Insert

    ENSDARG00000024546 (pla2g4aa Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pEF_dCas9

    Plasmid

    #68416

    Purpose

    Transient expression of Sp-dCas9 in mammalian cells, under an EF1-alpha promoter.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A/H841A

    Promoter

    human EF1[alpha]

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pUBC_V5_MS2-mCherry

    Plasmid

    #68420

    Purpose

    Transient expression of humanized MS2 (V75E/A81G), fused to mCherry in mammalian cells, under a UBC promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    MS2
    

    Use

    Synthetic Biology

    Tags

    V5 and mCherry

    Expression

    Mammalian

    Mutation

    MS2: V75E/A81G

    Promoter

    human UbC

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAM5411

    Plasmid

    #132663

    Purpose

    RSF1010-based broad host-range plasmid with RK2 bom site and pUC origin expressing YFP and Sp/Sm resistance

    Depositor

    Susan Golden

    Article

    Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

    Inserts

    aadA
    YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PaadA and PconII

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. MLV Gag-RFP

    Plasmid

    #1814

    Depositor

    Walther Mothes

    Article

    Sherer et al Traffic 2003 Nov;4(11):785-801.

    Insert

    Gag (gag MuLV)
    

    Use

    Tags

    RFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 19, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCambia-PUP-IT

    Plasmid

    #186478

    Purpose

    Expresses POI-PUP-IT fusion in Plants

    Depositor

    Jen Sheen

    Article

    Ye et al Nature. 2022 Sep 14. pii: 10.1038/s41586

    Insert

    FLAG-Pup(E)
    

    Use

    Tags

    Triple Flag Tag

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLenti-dCas9-KRAB-gRNA-TRE-blast

    Plasmid

    #201152

    Purpose

    Lentiviral expression of S. pyogenes dead Cas9 (dCas9/dSpCas9/SpdCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

    Depositor

    Moshe Szyf

    Inserts

    dCas9-KRAB
    gRNA-TRE
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mutation

    gRNA sequence: TACGTTCTCTATCACTGAT

    Promoter

    Available since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. NES-Halo

    Plasmid

    #200201

    Purpose

    Expression of 3X HIV NES

    Depositor

    Sheila Teves

    Article

    Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

    Insert

    3X HIV Rev NES-Halo
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    None

    Promoter

    EF_1alpha

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Friend MLV Env YFP

    Plasmid

    #1820

    Depositor

    Walther Mothes

    Article

    Sherer et al Traffic 2003 Nov;4(11):785-801.

    Insert

    Envelope (env MuLV)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 19, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA-KIRIN1-GR

    Plasmid

    #107418

    Purpose

    Mammalian cell expression plasmid for potassium ion raitometric indicator KIRIN1-GR

    Depositor

    Robert Campbell

    Article

    Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

    Insert

    KIRIN1-GR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge