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pTriEx4-Rac1-2G

Plasmid

#66110

Purpose

Second generation Rac1 FRET biosensor

Depositor

Olivier Pertz

Article

Fritz et al Dev Cell. 2015 Sep 29. pii: S1534-5807(1

Insert

Second generation Rac1 FRET biosensor

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pTriEx4-Rac1-2G

Plasmid

#66110

Purpose

Second generation Rac1 FRET biosensor

Depositor

Olivier Pertz

Article

Fritz et al Dev Cell. 2015 Sep 29. pii: S1534-5807(1

Insert

Second generation Rac1 FRET biosensor

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mCherry-mTrim21

Plasmid

#105522

Purpose

T7 promotor drives in vitro transcription of mCherry-tagged wildtype mouse TRIM21 mRNA

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Use

In vitro transcription

Tags

mCherry

Promoter

T7

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mCherry-mTrim21

Plasmid

#105522

Purpose

T7 promotor drives in vitro transcription of mCherry-tagged wildtype mouse TRIM21 mRNA

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Use

In vitro transcription

Tags

mCherry

Promoter

T7

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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Fuw-dCas9-Tet1CD-P2A-BFP

Plasmid

#108245

Purpose

Lentiviral vector to express dCas9-Tet1CD-P2A-tagBFP

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

Insert

dCas9-Tet1CD-HA-P2A-BFP

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

dCas9-D10A, dCas9-H840A

Promoter

Ubc

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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Fuw-dCas9-Tet1CD-P2A-BFP

Plasmid

#108245

Purpose

Lentiviral vector to express dCas9-Tet1CD-P2A-tagBFP

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

Insert

dCas9-Tet1CD-HA-P2A-BFP

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

dCas9-D10A, dCas9-H840A

Promoter

Ubc

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pLM-mCerulean-cMyc

Plasmid

#23244

Depositor

Michel Sadelain

Article

Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

Insert

mCerulean-2A-MYC (MYC synthetic fluorescent protein, Human)

Use

Lentiviral

Available Since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only

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pLM-mCerulean-cMyc

Plasmid

#23244

Depositor

Michel Sadelain

Article

Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

Insert

mCerulean-2A-MYC (MYC Human, synthetic fluorescent protein)

Use

Lentiviral

Available Since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only

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pLM-mCherry-Klf4

Plasmid

#23243

Depositor

Michel Sadelain

Article

Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

Insert

mCherry_2A_KLF4 (KLF4 synthetic fluorescent protein, Human)

Use

Lentiviral

Available Since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only

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pLM-mCherry-Klf4

Plasmid

#23243

Depositor

Michel Sadelain

Article

Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

Insert

mCherry_2A_KLF4 (KLF4 Human, synthetic fluorescent protein)

Use

Lentiviral

Available Since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only

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CMV-Mito4x-GCaMP6f

Plasmid

#127870

Purpose

Fluorescent reporter for mitochondrial matrix Ca2+

Depositor

Timothy Ryan

Article

Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

Insert

4 repeats of COX8 signal peptide plus GCaMP6f

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

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CMV-Mito4x-GCaMP6f

Plasmid

#127870

Purpose

Fluorescent reporter for mitochondrial matrix Ca2+

Depositor

Timothy Ryan

Article

Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

Insert

4 repeats of COX8 signal peptide plus GCaMP6f

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

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Twitch-2B pcDNA3

Plasmid

#49531

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-2B

Tags

ECFP and cpCit174

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

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Twitch-2B pcDNA3

Plasmid

#49531

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-2B

Tags

ECFP and cpCit174

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

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METTL3 shRNA 2 tet pLKO puro

Plasmid

#162984

Purpose

Tet-inducible shRNA targeting human METTL3 #2

Depositor

Mark Rubin

Article

Cotter et al Mol Cancer Res. 2021 Jun 4. pii: 1541-77

Insert

methyltransferase like 3 (METTL3 Human)

Use

Lentiviral

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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METTL3 shRNA 2 tet pLKO puro

Plasmid

#162984

Purpose

Tet-inducible shRNA targeting human METTL3 #2

Depositor

Mark Rubin

Article

Cotter et al Mol Cancer Res. 2021 Jun 4. pii: 1541-77

Insert

methyltransferase like 3 (METTL3 Human)

Use

Lentiviral

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pSLCAR-CD19-CD3z

Plasmid

#135993

Purpose

Modular CD3z alone CAR backbone, For Transient Expression or Lentiviral Production

Depositor

Scott McComb

Article

Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

Insert

FMC63-3z-P2A-EGFP (CD247, CD28 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a-short

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pSLCAR-CD19-CD3z

Plasmid

#135993

Purpose

Modular CD3z alone CAR backbone, For Transient Expression or Lentiviral Production

Depositor

Scott McComb

Article

Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

Insert

FMC63-3z-P2A-EGFP (CD247, CD28 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a-short

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-APOBEC1-YTH

Plasmid

#131636

Purpose

Expresses APOBEC1 fused to the YTH domain

Depositor

Kate Meyer

Article

Meyer et al Nat Methods. 2019 Sep 23. pii: 10.1038/s

Insert

APOBEC1 fused to YTH domain (YTHDF2 Human, Rat)

Tags

HA

Expression

Mammalian

Available Since

Oct. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-APOBEC1-YTH

Plasmid

#131636

Purpose

Expresses APOBEC1 fused to the YTH domain

Depositor

Kate Meyer

Article

Meyer et al Nat Methods. 2019 Sep 23. pii: 10.1038/s

Insert

APOBEC1 fused to YTH domain (YTHDF2 Human, Rat)

Tags

HA

Expression

Mammalian

Available Since

Oct. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge