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Plasmid#84933PurposecAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R739A should increase affinity for cAMP without affecting specificity.DepositorInsertcAMPr Y205A R739A
UseTagsExpressionInsectMutationPKA-C Y205A, PKA-R R739APromoter5X UASAvailable sinceMarch 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUASTattb-cAMPr Y205A, R626A
Plasmid#84932PurposecAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R626A should decrease affinity for cAMP without affecting specifity.DepositorInsertcAMPr Y205A R626A
UseTagsExpressionInsectMutationPKA-C Y205A, PKA-R R626APromoter5X UASAvailable sinceMarch 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-mROCK1-delta7
Plasmid#101295PurposeMammalian expression of Rock1-GFP for the study of localisation signaling.DepositorInsertRock1 (Rock1 Mouse)
UseTagsExpressionMammalianMutation727-1354 aaPromoterCMVAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-rhodopsin-CTS
Plasmid#86071PurposeA bacterial expression plasmid encoding rhodopsin ciliary targeting signal with N-terminus GST-tag.DepositorInsertRhodopsin (RHO Bovine)
UseTagsGSTExpressionBacterialMutationcontains the region from 309-348 of RhodopsinPromotertacAvailable sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAV0764
Plasmid#133520PurposeExpression of mCherry targeted to the endoplasmic reticulum with a signal sequence and a retention signal (AHDL)DepositorInsertpLys3(BstZ17I)-p(BiP)-SignalSequenceBiP-mCherry-AHDL-bsdMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0773
Plasmid#133519PurposeExpression of sfGFP targeted to the endoplasmic reticulum with a signal sequence and a retention signal (AHDL)DepositorInsertpLys3(BstZ17I)-p(BiP)-SignalSequenceBiP-sfGFP-AHDL-bsdMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJAZ10::LUC
Plasmid#141172PurposeLuminescent reporter for jasmonic acid signalingDepositorInsertAtJAZ10 promoter
UseLuciferaseTagsExpressionPlantMutationPromoterAtJAZ10, AT5G13220Available sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-mROCK1-delta5
Plasmid#101293PurposeMammalian expression of Rock1-GFP for the study of localisation signaling.DepositorInsertRock1 (Rock1 Mouse)
UseTagsExpressionMammalianMutation375-727 aaPromoterCMVAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXylA-agrCA-IV
Plasmid#53438Purposexylose-dependent expression of the type-IV agrC and type-I agrA genes of S. aureusDepositorInsertagrCA locus
UseSynthetic BiologyTagsnoneExpressionBacterialMutation"MVQTS" sequence added to the N-terminu…PromoterPxylAAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pWRKY70::LUC
Plasmid#141171PurposeLuminescent reporter for salicylic acid signalingDepositorInsertAtWRKY70 promoter
UseLuciferaseTagsExpressionPlantMutationPromoterAtWRKY70, AT3G56400Available sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV-Fast-1 H83R
Plasmid#16522DepositorInsertFAST-1 H83R (FOXH1 Human)
UseTagsExpressionMammalianMutationH83R mutantPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCS2 YFP-Smo-Weyer's
Plasmid#41014DepositorInsertsmoothened homolog (Smo Mouse)
UseTagsEVC2 (aa 1177-1220) and YFPExpressionMammalianMutationYFP inserted after Smo signal sequence. Transfer…PromoterAvailable sinceNov. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
GST-FAST-FH
Plasmid#16524DepositorInsertFAST-1 (FH domain) (FOXH1 Human)
UseTagsGSTExpressionBacterialMutationamino acids 1-150. Forkhead domain.PromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSCKtheoRaj12m
Plasmid#69943PurposepSTC2,TheoHHAzRaj12mut,cisRaj12-sfGFP, kanRDepositorInsertpSCKtheoRaj12m
UseSynthetic Biology; Psc101 origin of replicationTagsExpressionMutationPromoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAC-EsaR-I70V/D91G
Plasmid#47644PurposepAC-EsaR with a208g and a272g mutations in esaRDepositorInsertesaR-I70V/D91G
UseSynthetic BiologyTagsExpressionBacterialMutationChanged Isoleucine 70 to Valine and Aspartic acid…PromoterPlacAvailable sinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
GST-PC-1-CTS
Plasmid#86067PurposeA bacterial expression plasmid encoding polycystin-1 ciliary targeting signal with N-terminus GST-tag.DepositorInsertPolycystin-1 (PKD1 Human)
UseTagsGSTExpressionBacterialMutationcontains the region from 4297-4303PromotertacAvailable sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSCKtheoRaj12
Plasmid#69942PurposepSTC2,TheoHHAzRaj12,cisRaj12-sfGFP, kanRDepositorInsertTheoHHAzRaj12,cisRaj12,sfGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSCKtheoRaj11
Plasmid#69939PurposepSTC1,TheoHHAzRaj11,cisRaj11-sfGFP, kanRDepositorInsertTheoHHAzRaj11,cisRaj11,sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSCKbreak12
Plasmid#69938PurposepSTC2,cisRaj12-sfGFP, KanRDepositorInsertCisRaj12-sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAC-EsaR-D91G/V220A
Plasmid#47648PurposepAC-EsaR with a272g and c659t mutations in esaRDepositorInsertesaR-D91G/V220A
UseSynthetic BiologyTagsExpressionBacterialMutationChanged Aspartic acid 91 to Glycine, and Valine 2…PromoterPlacAvailable sinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only