We narrowed to 17,881 results for: por
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Plasmid#109423PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody with a TEV site. VHH-tev-std is flanked by an N-terminal T7 epitope, and a C-terminal cassette composed of HA, BAP and a His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, TEV cleavage site, HA, BAP and His6 epitope
TagsBAP, HA, His6, and T7ExpressionBacterialPromoterT7Available SinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG-KPNA2
Plasmid#192306PurposeGateway entry vector for an inducible 3xFLAG-KPNA2DepositorAvailable SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-EF1a-DIO-GRAB_r5-HT1.0
Plasmid#208725PurposeExpresses the red 5-HT sensor GRAB_r5-HT1.0 in neurons in the presence of Cre recombinaseDepositorInsertRed fluorescent 5-HT sensor GRAB_r5-HT1.0
UseAAVPromoterEF1aAvailable SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
ATP9A-Tol2
Plasmid#113385Purposeevaluate FOXA1 DIV motif enhancer activity near 50 kb of ATP9A geneDepositorInsertATP9A-enhancer (ATP9A Human)
UseTol2 reporterAvailable SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330-sgRNA_Ago2_1
Plasmid#73529PurposeExpresses a human codon-optimized SpCas9 and sgRNA targeting Ago2DepositorInsertAGO2
UseCRISPRExpressionMammalianPromoterhU6Available SinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330-sgRNA_Ago2_2
Plasmid#73530PurposeExpresses a human codon-optimized SpCas9 and sgRNA targeting Ago2DepositorInsertAGO2
UseCRISPRExpressionMammalianPromoterhU6Available SinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPN062
Plasmid#91595PurposeExpress sgRNA targeting human DFNA5DepositorAvailable SinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPN063
Plasmid#91596PurposeExpress sgRNA targeting human DFNA5DepositorAvailable SinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_PfeAQ482A
Plasmid#128945Purposeexpresses Q482A mutant of PfeA in E. coliDepositorInsertferric enterobactin receptor
TagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-75), …PromoterT7Available SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
ALARM NMR
Plasmid#90209PurposeExpresses the human La antigen for use in ALARM NMRDepositorInsertLa antigen (SSB Human)
Tags6xHisExpressionBacterialMutationcontains amino acids 100-324PromoterT7 promoterAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
FLS2-YFPc
Plasmid#87166Purposefluorescence complementation assayDepositorAvailable SinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSF3-Flag-Cbir-Cdc25C WT_Myc-Nbir-GFP
Plasmid#90012PurposeSplit-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C and N-terminally tagged NBir[2-256]-GFPDepositorInsertsCBirA*-Cdc25C
NBirA*-GFP
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianPromoterCMVAvailable SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-MCU-S259A
Plasmid#31728DepositorAvailable SinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
QSOX1-Tol2
Plasmid#113383Purposeevaluate FOXA1 DIV motif enhancer activity near 50 kb of QSOX1 geneDepositorInsertQSOX1-enhancer (QSOX1 Human)
UseTol2 reporterAvailable SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9-Puro HLAG-2A-sgRNA
Plasmid#182551PurposeCas9 from S.pyogenes with 2A-Puro, and the 2A-sgRNA to targeting exon 2 of human HLA-G geneDepositorInserthSpCas9-2A-Puro-HLAG-2A-sgRNA
UseCRISPRExpressionMammalianPromoterCbhAvailable SinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-IFT27
Plasmid#218722PurposeExpresses N-terminally EGFP-tagged IFT27 in mammalian cellsDepositorAvailable SinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
FLS2-YFPn
Plasmid#87165Purposefluorescence complementation assayDepositorAvailable SinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro Xlone SOX17
Plasmid#179838PurposeDoxycycline-inducible expression of human SOX17DepositorInsertSOX17 (SOX17 Human)
UseAAV, CRISPR, Synthetic Biology, and TALEN ; Donor…ExpressionMammalianPromoterTRE3GSAvailable SinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pCL026 AAVS1-puroR-9xTet0-pPGK-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
Plasmid#196545PurposeDonor plasmid for integrating PGK promoter-driven dual surface marker/fluorescent reporter at AAVS1 locusDepositorInsertSurface marker and Citrine reporter
UseSynthetic BiologyExpressionMammalianAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-NTerm 6xHis-zmADH-T500
Plasmid#92228PurposeExpression of zmADH using the p70a promoter and UTR1DepositorInsertNTerm 6xHis Tagged zmADH
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialPromoterp70aAvailable SinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
1K245 GLUT1-GFP
Plasmid#200097Purposehuman GLUT1 with a single cytosolic lysine (K245) and C-terminal GFP tag in pQCXIP vectorDepositorInsertGLUT1 (SLC2A1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationK-->R mutations of amino acids 6, 7, 225, 229,…PromoterCMVAvailable SinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-GRAB_g5-HT3.0mut
Plasmid#208724PurposeExpresses the green 5-HT sensor GRAB_g5-HT3.0mut in neurons in the presence of Cre recombinaseDepositorInsertGreen fluorescent 5-HT sensor GRAB_g5-HT3.0mut
UseAAVPromoterEF1aAvailable SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AMPKsub(TA)-YPet-NES
Plasmid#84636PurposeEncodes negative-control C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertAMPKsub(TA)-YPet-NES
Tags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable SinceApril 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC6A2
Plasmid#131891PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC6A2 (SLC6A2 Human)
ExpressionMammalianAvailable SinceOct. 9, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAS_4xG1 PylT FLAG-G1 PylRS
Plasmid#154768Purposeplasmid with 4xG1 PylT cassette and G1 PylRS for amber suppression in transient or stable, piggybac-mediated, integrationDepositorInsertG1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
TagsFLAGExpressionMammalianPromoterEF1Available SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
shFGF2#2
Plasmid#157668PurposeTet inducible knockdown of FGF2DepositorAvailable SinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
LightR-bRaf-mVenus
Plasmid#162154PurposeExpresses LightR-bRaf-mVenus in mammalian cellsDepositorInsertLightR-bRaf-mVenus
TagsmVenusExpressionMammalianMutationValine 600 is substituted with Glutamic acidPromoterCMVAvailable SinceDec. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF3B tail 391-747
Plasmid#134615PurposeMammalian expression of mouse KIF3B 391-747DepositorInsertKIF3B (Kif5b Mouse)
TagseGFPExpressionMammalianMutationWT with deletion of the 390 N-terminal residues.PromoterChicken Beta actinAvailable SinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only