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pCas9-CtIP

Plasmid

#109403

Purpose

Expresses Cas9 fusion to human CtIP protein

Depositor

Jean-Paul Concordet

Article

Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

Insert

CtIP (RBBP8 Human)

Mutation

full length CtIP

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Myc2- Hs TTP WT

Plasmid

#107008

Purpose

Expresses epitope-tagged wild-type human TTP

Depositor

Julian Downward

Article

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

tristetraprolin (ZFP36 Human)

Tags

Double Myc peptide epitope tag

Expression

Mammalian

Available Since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST53-LRRK2-R1441C

Plasmid

#25046

Depositor

Mark Cookson

Article

Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

Insert

LRRK2 (LRRK2 Human)

Tags

GFP

Expression

Mammalian

Mutation

Arginine 1441 mutated to Cysteine

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-SOX4

Plasmid

#110360

Purpose

Mammalian expression of FLAG-tagged SOX4

Depositor

Carlos Moreno

Article

Liu et al Cancer Res. 2006 Apr 15;66(8):4011-9. do

Insert

SOX4 (SOX4 Human)

Tags

FLAG

Expression

Mammalian

Mutation

lacking 5' UTR

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCXN2-FLAG-AT2R-CFP

Plasmid

#101660

Purpose

Expresses AT2R with FLAG tag and CFP in mammalian cells.

Depositor

Yusuke Ohba

Article

Inuzuka et al Sci Rep. 2016 Feb 9;6:21613. doi: 10.103

Insert

Angiotensin II type 2 receptor (AGTR2 Human)

Tags

ECFP and FLAG

Expression

Mammalian

Promoter

CAG promoter

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pCas9-HE

Plasmid

#109400

Purpose

Expresses Cas9 fusion to HE (HDR-enhancer) domain of human CtIP protein

Depositor

Jean-Paul Concordet

Article

Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

Insert

HDR-Enhancer domain of human CtIP protein (RBBP8 Human)

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-tetOn-IRES-G418 (EtO)

Plasmid

#84776

Purpose

Second generation lenti tetracycline operator (on)

Depositor

Fred Gage

Article

Mertens et al Cell Stem Cell. 2015 Oct 6. pii: S1934-5

Insert

TET OPERATOR

Use

Lentiviral

Promoter

pTIGHT

Available Since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A STING V155M

Plasmid

#102591

Purpose

Lentivector to express STING V155M and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

V155M

Promoter

SFFV

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCas9-Geminin

Plasmid

#109401

Purpose

Expresses Cas9 fusion to Geminin degron domain

Depositor

Jean-Paul Concordet

Article

Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

Insert

Geminin (GMNN Human)

Mutation

Geminin degron fragment

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pTU1-A-pdh_RiboJ_mCherry_Bba_B0015

Plasmid

#107581

Purpose

B. megaterium DSM319 pdh promoter, mCherry (Bacillus codon optimised) for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)

Depositor

Paul Freemont

Article

Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

Insert

mCherry

Use

Synthetic Biology

Expression

Bacterial

Promoter

pdh promoter Bacillus megaterium DSM319

Available Since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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pMP71-HLA-B0702-His

Plasmid

#104850

Purpose

HLA-B0702 his tagged

Depositor

Sébastien Walchli

Article

Lampen et al Immunohorizons. 2018 Jul 2;2(6):172-184.

Insert

major histocompatibility complex, class I, B (HLA-B Human)

Use

Retroviral

Tags

his

Expression

Mammalian

Promoter

LTR

Available Since

Dec. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCV-hygro-STING V155M

Plasmid

#102599

Purpose

Express STING V155M for IFN-beta Luciferase reporter assay.

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

V155M

Available Since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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Myc-Nix

Plasmid

#100795

Purpose

Mammalian expression of Nix (Bnip3L)

Depositor

Joseph Gordon

Article

Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

Insert

BCL2 interacting protein 3 like (BNIP3L Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-CIB1-mCerulean-MP

Plasmid

#58366

Purpose

For use in light-induced protein inactivation through the interaction with CRY2

Depositor

Won Do Heo

Article

Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

Inserts

CIB1[1-147] (CIB1 Mustard Weed)
Camk2a[315-478] (Camk2a Rat)

Tags

mCerulean

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pHR-human U6-sgRNA-EF1Alpha-puro-T2A-BFP

Plasmid

#118594

Purpose

Parental vector for the CRISPRa libraries. Expresses an sgRNA from the human U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

Depositor

Stanley Qi

Article

Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

Insert

sgRNA/Puro-T2A-BFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF1Alpha

Available Since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs-ms-c-Myc-L420P

Plasmid

#50776

Purpose

retroviral expression of mouse c-Myc L420P mutant

Depositor

Shinya Yamanaka

Article

Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

c-Myc L420P (MYC, Myc Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

L420P (does not bind to Max)

Available Since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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lentiCas9-Venus

Plasmid

#70267

Purpose

Expresses human codon-optimized S. pyogenes Cas9 protein and Venus reporter from EFS promoter. Lentiviral backbone.

Depositor

Daniel Bauer

Article

Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature

Inserts

Cas9
Venus Reporter

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EFS-NS and P2A

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST53-LRRK2-3XKD

Plasmid

#25049

Depositor

Mark Cookson

Article

Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

Insert

LRRK2 (LRRK2 Human)

Tags

GFP

Expression

Mammalian

Mutation

"Kinase Dead" Lysine 1906 mutated to A…

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-HtrA2-FLAG

Plasmid

#15938

Depositor

L. Miguel Martins

Article

Plun-Favreau et al Nat Cell Biol. 2007 Nov;9(11):1243-52. d

Insert

HtrA2 (HTRA2 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 5, 2007

Availability

Academic Institutions and Nonprofits only

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pGEN25-Ub Hs

Plasmid

#83232

Purpose

pHis-par2-Ub Hs

Depositor

Gali Prag

Article

Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

Insert

Ubiqutin (UBC Human)

Tags

His

Expression

Bacterial

Available Since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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