We narrowed to 6,251 results for: nls

1. AXBEv2

   Plasmid

   #204606

   Purpose

   Mammalian expression of evolved adenine transversion base editor AXBEv2

   Depositor

   Dali Li

   Article

   Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

   Insert

   bpNLS-TadA-8e-nSpCas9-mAAG (R165E/Y179F)-bpNLS-P2A-GFP
   

   Use

   CRISPR

   Tags

   6*His

   Expression

   Mammalian

   Mutation

   mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)

   Available Since

   Aug. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-TO-NGN2-EMX1 BSD-mApple

   Plasmid

   #182311

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 and EMX1 expression, bsd selection, mApple

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Inserts

   hNGN2-P2A-EMX1 (NEUROG2, EMX1 Human)
   bsd-T2A-mycNLS-mApple
   

   Tags

   T2A-mycNLS-mApple

   Expression

   Mammalian

   Promoter

   EF1a and TRE3G

   Available Since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. ACBE

   Plasmid

   #204607

   Purpose

   Mammalian expression of adenine-to-cytosine base editor ACBE

   Depositor

   Dali Li

   Article

   Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

   Insert

   bpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
   

   Use

   CRISPR

   Tags

   6*His

   Expression

   Mammalian

   Mutation

   mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)

   Available Since

   Aug. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. miniCGBE1 (pBM1219)

   Plasmid

   #140253

   Purpose

   CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

   Insert

   BE4max(R33A)-ΔUGI
   

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1, D10A in Cas9

   Promoter

   CMV

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-Target-ACEmax

   Plasmid

   #139105

   Purpose

   Mammalian Target-ACEmax expression vector (pSI-625)

   Depositor

   Nozomu Yachie

   Article

   Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. lentiBE3-SpRY-Blast

   Plasmid

   #199303

   Purpose

   Expresses SpRY Cas9 nickase cytosine base editor FNLS-BE3 and blasticidin resistance

   Depositor

   Neville Sanjana

   Article

   Morris et al Science. 2023 May 19;380(6646):eadh7699.

   Insert

   FNLS-BE3-SpRY
   

   Use

   CRISPR and Lentiviral

   Tags

   2A tag

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRD406

   Plasmid

   #163111

   Purpose

   Expression of mNeonGreen_H-NSdbd in eukaryotes for visualisation of nuclear DNA

   Depositor

   Remus Dame

   Article

   Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

   Insert

   NLS_mNeonGreen_H-NSdbd_NLS
   

   Use

   Eukaryotic expression

   Expression

   Mammalian

   Promoter

   CMV promoter; T7 promoter

   Available Since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRD399

   Plasmid

   #163106

   Purpose

   Expression of mEGFP_H-NSdbd in eukaryotes for visualisation of nuclear DNA

   Depositor

   Remus Dame

   Article

   Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

   Insert

   NLS_mEGFP_H-NSdbd_NLS
   

   Use

   Eukaryotic expression

   Expression

   Mammalian

   Promoter

   CMV promoter; T7 promoter

   Available Since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. miniCGBE1-NG (pBM1182)

   Plasmid

   #140257

   Purpose

   CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

   Insert

   BE4max(R33A)_NG-ΔUGI
   

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

   Promoter

   CMV

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-ACBEmax

    Plasmid

    #139106

    Purpose

    Mammalian ACBEmax expression vector (pSI-632)

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-rAPOBEC1-2xUGI-SV40NLS
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Polymerase expression - pCMV-Phi29(+exo) (LM2759)

    Plasmid

    #208964

    Purpose

    Unfused Phi29 DNA polymerase (+exo), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    Phi29(+exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Polymerase expression - pCMV-Phi29 (LM2708)

    Plasmid

    #208965

    Purpose

    Unfused Phi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    Phi29-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    Phi29(-exo;D169A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. SunTagCACTA1g2-22aa-TET1cd

    Plasmid

    #106437

    Purpose

    SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the CACTA1 promoter

    Depositor

    Steven Jacobsen

    Article

    Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

    Insert

    CACTA1gRNA2_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. Polymerase expression - pCMV-B103 (LM2907)

    Plasmid

    #208968

    Purpose

    Unfused B103 DNA polymerase, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    B103-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRD398

    Plasmid

    #163105

    Purpose

    Expression of mCherry_H-NSdbd in eukaryotes for visualisation of nuclear DNA

    Depositor

    Remus Dame

    Article

    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

    Insert

    NLS_mCherry_H-NSdbd_NLS
    

    Use

    Eukaryotic expression

    Expression

    Mammalian

    Promoter

    CMV promoter; T7 promoter

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRD190

    Plasmid

    #163100

    Purpose

    Expression of mEos3.2 in eukaryotes to stain the nuclear compartment

    Depositor

    Remus Dame

    Article

    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

    Insert

    NLS_mEos3.2_NLS
    

    Use

    Eukaryotic expression

    Expression

    Mammalian

    Promoter

    CMV promoter; T7 promoter

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRD397

    Plasmid

    #163104

    Purpose

    Expression of mTurquoise2_H-NSdbd in eukaryotes for visualisation of nuclear DNA

    Depositor

    Remus Dame

    Article

    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

    Insert

    NLS_mTurquoise2_H-NSdbd_NLS
    

    Use

    Eukaryotic expression

    Expression

    Mammalian

    Promoter

    CMV promoter; T7 promoter

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. TadA-8e-eIscBn-C-HMG-D

    Plasmid

    #227145

    Purpose

    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e and HMG-D domain in C terminus driven by CMV promoter

    Depositor

    Dali Li

    Article

    Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e

    Insert

    bpNLS-TadA-8e-linker-nOgeuIscB-v3-linker-HMG-D-bpNLS
    

    Mutation

    D96R/E84R/V159R/H339A

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. TadA-8e-eIscBn-N-HMG-D

    Plasmid

    #227143

    Purpose

    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e and HMG-D domain in N terminus driven by CMV promoter

    Depositor

    Dali Li

    Article

    Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e

    Insert

    bpNLS-HMG-D-linker-TadA-8e-linker-nOgeuIscB-v3-bpNLS
    

    Mutation

    D96R/E84R/V159R/H339A

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. HEpl016

    Plasmid

    #179991

    Purpose

    pCBh_3xFLAG_NLS_SiCas12i_NLS_pA_pCMV_mCherry_pU6_crRNA_BbsI: vector for encoding a human codon-optimized SiCas12i driven by CBh promoter and tagmCherry driven by CMV promoter

    Depositor

    Yingsi Zhou

    Article

    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

    Insert

    SiCas12i
    

    Tags

    3xFlag

    Expression

    Mammalian

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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