We narrowed to 69,671 results for: cas

1. lenti-dCas9-VP64-BFP

   Plasmid

   #196711

   Purpose

   CRISPR-activation. dCas9-VP64 with TagBFP for sorting

   Depositor

   Bernhard Schmierer

   Insert

   dCas9-VP64-T2A-TagBFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mutation

   D10A, N863A

   Promoter

   EF1a

   Available since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. lenti-dCas9-VP64-BFP

   Plasmid

   #196711

   Purpose

   CRISPR-activation. dCas9-VP64 with TagBFP for sorting

   Depositor

   Bernhard Schmierer

   Insert

   dCas9-VP64-T2A-TagBFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mutation

   D10A, N863A

   Promoter

   EF1a

   Available since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. eSpCas9(1.1)-T2A-Puro

   Plasmid

   #101039

   Purpose

   Enhanced SpCas9(1.1) with puromycin resistance gene via T2A linker.

   Depositor

   Andrea Németh

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   3xFLAG and T2A-Puro

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Nov. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. eSpCas9(1.1)-T2A-Puro

   Plasmid

   #101039

   Purpose

   Enhanced SpCas9(1.1) with puromycin resistance gene via T2A linker.

   Depositor

   Andrea Németh

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   3xFLAG and T2A-Puro

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Nov. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pspCas9-HF-tetra-com-vector

   Plasmid

   #132555

   Purpose

   Vector plasmid expressing high fidelity spCas9 (R691A) and sgRNA scaffold with com replacing the Tetraloop.

   Depositor

   Baisong Lu

   Article

   Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

   Inserts

   High fidelity Cas9
   sgRNA scaffold with com replacing the Tetraloop
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pspCas9-HF-tetra-com-vector

   Plasmid

   #132555

   Purpose

   Vector plasmid expressing high fidelity spCas9 (R691A) and sgRNA scaffold with com replacing the Tetraloop.

   Depositor

   Baisong Lu

   Article

   Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

   Inserts

   High fidelity Cas9
   sgRNA scaffold with com replacing the Tetraloop
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCE046-SiT-Cas12a-[Repr]

   Plasmid

   #128132

   Purpose

   Express both AsCas12a fused to [Repr] repressor domain and CRISPR arrays on a single transcript

   Depositor

   Randall Platt

   Article

   Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

   Insert

   AsCas12a-[Repr]-Triplex
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCE046-SiT-Cas12a-[Repr]

   Plasmid

   #128132

   Purpose

   Express both AsCas12a fused to [Repr] repressor domain and CRISPR arrays on a single transcript

   Depositor

   Randall Platt

   Article

   Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

   Insert

   AsCas12a-[Repr]-Triplex
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDF0317 HvsCas7-11 mammalian expression

   Plasmid

   #172511

   Purpose

   Encodes human codon optimized HvsCas7-11 protein alone for expression in mammalian cells

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

   Insert

   HvsCas7-11 mammalian expression
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDF0317 HvsCas7-11 mammalian expression

    Plasmid

    #172511

    Purpose

    Encodes human codon optimized HvsCas7-11 protein alone for expression in mammalian cells

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    HvsCas7-11 mammalian expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCas9-EcRT-GAL-Hyg

    Plasmid

    #232091

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Insert

    SpCas9 and Ec86 reverse transcriptase
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Ec86 reverse transcriptase is codon optimized for…

    Promoter

    GAL1-GAL10

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCas9-EcRT-GAL-Hyg

    Plasmid

    #232091

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Insert

    SpCas9 and Ec86 reverse transcriptase
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Ec86 reverse transcriptase is codon optimized for…

    Promoter

    GAL1-GAL10

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MO91-dCas9-AID(P182X)

    Plasmid

    #83260

    Purpose

    Expresses fusion of human codon-optimized S. pyogenes dCas9 (D10A, H840A ) protein with a human AID mutant protein (p182X). MSCV Retroviral backbone.

    Depositor

    Xing Chang

    Article

    Ma et al Nat Methods. 2016 Dec;13(12):1029-1035.

    Insert

    dCas9-AID P182X
    

    Use

    Retroviral

    Tags

    3xFLAG-NLS and FLAG

    Expression

    Mammalian

    Mutation

    D10A and H840A in Cas9; P182X in AID

    Promoter

    MSCV

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

14. MO91-dCas9-AID(P182X)

    Plasmid

    #83260

    Purpose

    Expresses fusion of human codon-optimized S. pyogenes dCas9 (D10A, H840A ) protein with a human AID mutant protein (p182X). MSCV Retroviral backbone.

    Depositor

    Xing Chang

    Article

    Ma et al Nat Methods. 2016 Dec;13(12):1029-1035.

    Insert

    dCas9-AID P182X
    

    Use

    Retroviral

    Tags

    3xFLAG-NLS and FLAG

    Expression

    Mammalian

    Mutation

    D10A and H840A in Cas9; P182X in AID

    Promoter

    MSCV

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

15. pCas9/VRQR-sgRNA (BbsI)

    Plasmid

    #129725

    Purpose

    Expressing SpCas9/VRQR mutant and sgRNA for target sequence with NGA PAM

    Depositor

    Elina Ikonen

    Article

    Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCas9/VRQR-sgRNA (BbsI)

    Plasmid

    #129725

    Purpose

    Expressing SpCas9/VRQR mutant and sgRNA for target sequence with NGA PAM

    Depositor

    Elina Ikonen

    Article

    Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Pmec-18 caspase-3 (p12)::nz [TU#813]

    Plasmid

    #16082

    Depositor

    Martin Chalfie

    Article

    Chelur et al Proc Natl Acad Sci U S A. 2007 Feb 13. 1

    Insert

    caspase-3 (p12) :: nz (CASP3 Human)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 23, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Pmec-18 caspase-3 (p12)::nz [TU#813]

    Plasmid

    #16082

    Depositor

    Martin Chalfie

    Article

    Chelur et al Proc Natl Acad Sci U S A. 2007 Feb 13. 1

    Insert

    caspase-3 (p12) :: nz (CASP3 Human)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 23, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AID∆C-Cas9n-UGI-NLS

    Plasmid

    #198890

    Purpose

    Express AID(1-188) BE3-based base editor

    Depositor

    Reuben Harris

    Article

    Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

    Insert

    Actication induced deaminase (AICDA Human)
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 189-198

    Promoter

    CMV

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AID∆C-Cas9n-UGI-NLS

    Plasmid

    #198890

    Purpose

    Express AID(1-188) BE3-based base editor

    Depositor

    Reuben Harris

    Article

    Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

    Insert

    Actication induced deaminase (AICDA Human)
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 189-198

    Promoter

    CMV

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge