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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 5461 - 5480 of 6267 results

  7. pUCC001

    Plasmid
    #124451
    Purpose
    Multi-species CRISPR/Cas9 coexpression system, optimized for Golden Gate cloning of gRNA targets
    Depositor
    John Morrissey
    Article
    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97
    Insert
    HH-BsaI
    Expression
    Yeast
    Mutation
    gRNA expression cassette from pUDP002 modified to…
    Promoter
    TDH3 promoter from S.cerevisiae (already present …
    Available Since
    Sept. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    124451-plasmid-map-sequence-id-257763

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  10. p46Cpf1-OP2

    Plasmid
    #98592
    Purpose
    Produces lambda Red and Cpf1 for recombination and selection. The plasmid is combined with a donor plasmid (with crRNA and template) for rapid genome editing in E.coli.
    Depositor
    Qiong Wu
    Article
    Ao et al Front Microbiol. 2018 Oct 9;9:2307. doi:
    Insert
    FnCpf1
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    Codon optimized for E. coli
    Promoter
    araBAD
    Available Since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    98592-plasmid-map-sequence-id-201329

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  13. pFH6

    Plasmid
    #86555
    Purpose
    Subcloning of any sgRNA via BbsI sites. Note that there is an improved version of this plasmid (Addgene 105866) that results in up to 10x greater efficiency
    Depositor
    Andreas Weber
    Article
    Hahn et al Front Plant Sci. 2017 Jan 24;8:39. doi:
    Insert
    U6-26p::sgRNA scaffold
    Use
    CRISPR; Subcloning
    Promoter
    Arabidopsis U6-26 promoter
    Available Since
    May 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86555-plasmid-map-sequence-id-176427

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Showing: 5461 - 5480 of 6267 results