We narrowed to 6,119 results for: arl

1. pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (WT)

   Plasmid

   #196702

   Purpose

   Plasmid for transient mammalian expression of wild type RNase H1 tagged with 2xNLS and EGFP that can be used to specifically degrade the nuclear R-loops.

   Depositor

   Karlene Cimprich

   Article

   Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

   Insert

   human RNaseH1 wild type
   

   Expression

   Mammalian

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-dCas9-HA

   Plasmid

   #61355

   Purpose

   encodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Insert

   S.pyogenes dCas9 with c-terminal HA tag
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag, HA, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   CMV

   Available Since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-EF1a-Cre

   Plasmid

   #55636

   Purpose

   Expresses Cre in Mammalian Cells

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Has Service

   AAV Retrograde and AAV11

   Insert

   Cre
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJ204-loxP-EF1a-tagBFP-HygR-lox2272-BGHpA-siteT9-HDR

   Plasmid

   #154827

   Purpose

   Landing pad for Cre/lox-based recombinase-mediated cassette exchange (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted integration in CHO cells (site T9)

   Depositor

   Lise Marie Grav

   Article

   Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

   Inserts

   TagBFP
   HygR
   ZsGreen1-DR
   

   Use

   CRISPR and Cre/Lox

   Tags

   PEST

   Expression

   Mammalian

   Promoter

   CMV, EF1a, and SV40 early promoter

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJ204-loxP-EF1a-mCherry-NeoR-lox2272-BGHpA-siteA-HDR

   Plasmid

   #154826

   Purpose

   Landing pad for Cre/lox-based recombinase-mediated cassette exchange (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted integration in CHO cells (site A)

   Depositor

   Lise Marie Grav

   Article

   Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

   Inserts

   mCherry
   NeoR
   ZsGreen1-DR
   

   Use

   CRISPR and Cre/Lox

   Tags

   PEST

   Expression

   Mammalian

   Promoter

   CMV, EF1a, and SV40 early promoter

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Ef1a-DIO hChR2(E123A)-EYFP

   Plasmid

   #35507

   Purpose

   AAV expression of EF1a-driven, cre-dependent, hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

   Depositor

   Karl Deisseroth

   Article

   Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

   Insert

   hChR2
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E123A

   Promoter

   Ef1a

   Available Since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Ef1a-fDIO EYFP

   Plasmid

   #55641

   Purpose

   Flp-Dependent EYFP

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   EYFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Nov. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLENTI6.3/AR-GC-E2325

   Plasmid

   #85128

   Purpose

   exogenous AR expression

   Depositor

   Karl-Henning Kalland

   Article

   Olsen et al BMC Cancer. 2016 Jul 4;16:377. doi: 10.1

   Insert

   androgen receptor (AR Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA-dCas9-VP64

   Plasmid

   #47107

   Purpose

   Expresses inactivated S. pyogenes dCas9 (D10A, H840A) fused to VP64 transactivator domain in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

   Insert

   dCas9-VP64
   

   Use

   CRISPR

   Tags

   Flag, HA, SV40 NLS, and VP64

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive)

   Promoter

   CMV

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-UbC-VP64-dCas9-VP64

    Plasmid

    #232179

    Purpose

    Lentiviral cassette that expresses 2xVP64-dCas9 fusion in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

    Insert

    VP64-dCas9-VP64
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    hUbC

    Available Since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-EF1a-ArgiNLS-AausFP1

    Plasmid

    #220595

    Purpose

    Constitutive expression of a single-cell discriminating version of AausFP1 fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    AausFP1
    

    Use

    AAV

    Tags

    ArgiNLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pE-35S-miniSdd7-nCas9-PBE-GmPPO2

    Plasmid

    #204855

    Purpose

    For cytosine base editing using miniSdd7 at PPO2 site in soybean (Agrobacterium-mediated transformation)

    Depositor

    Caixia Gao

    Article

    Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

    Insert

    miniSdd7-nSpCas9(D10A)-UGI-mScarlet
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D10A in SpCas9

    Promoter

    35S, AtU6-26p

    Available Since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYTRW22K_7Ti1

    Plasmid

    #177293

    Purpose

    genomic integration of genes (inserted in I-SceI-site) via rrn-interposon with TcR and LacZ

    Depositor

    Karl-Erich Jaeger

    Article

    Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

    Insert

    lacZ
    

    Use

    Synthetic Biology; Yeast expression, rrn genomic …

    Expression

    Bacterial

    Available Since

    Aug. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-Ef1a-Coff/Fon-EYFP

    Plasmid

    #231927

    Purpose

    Cre-off/Flp-on EYFP under the Ef1a promoter

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    eYFP with introns
    

    Use

    AAV

    Promoter

    Ef1a

    Available Since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-EF1a-Flpo

    Plasmid

    #55637

    Purpose

    Expresses Flpo in Mammalian Cells

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Has Service

    AAV Retrograde, AAV1, and AAV2

    Insert

    Flpo
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-Ef1a-sRGECO

    Plasmid

    #137125

    Purpose

    Viral expression of sRGECO

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    sRGECO
    

    Use

    AAV

    Mutation

    E217D

    Promoter

    EF1a

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-EF1a-mCherry-IRES-Dre

    Plasmid

    #55633

    Purpose

    Expresses Dre in Mammalian Cells

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Inserts

    mCherry
    Dre
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Ef1a and Ef1a/IRES

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-U6-gRNA-UbC-DsRed-P2A-Bsr

    Plasmid

    #83919

    Purpose

    Lentiviral SpCas9-gRNA expression vector with DsRed-Express2-P2A-BlastR

    Depositor

    Charles Gersbach

    Article

    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

    Inserts

    DsRed-Express2
    Bsr
    

    Use

    CRISPR and Lentiviral

    Tags

    P2A

    Promoter

    hUbC

    Available Since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-Ef1a-dDIO hChR2(H134R)-EYFP

    Plasmid

    #55640

    Purpose

    Dre-Dependent ChR2-EYFP

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    hChR2(H134R)-EYFP
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Aug. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYTRW09K_0T5

    Plasmid

    #177281

    Purpose

    genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with TcR

    Depositor

    Karl-Erich Jaeger

    Article

    Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

    Insert

    Ptet-tetA(C)
    

    Use

    Synthetic Biology; Yeast expression, tn5 genomic …

    Expression

    Bacterial

    Available Since

    Aug. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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