We narrowed to 11,578 results for: VARS

1. pCMV-dCas12a-A3A-N57G

   Plasmid

   #193639

   Purpose

   Expression of base editor dCas12a-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   dLbCas12a-hA3A-N57G, EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   hAPOBEC3A-N57G

   Promoter

   CMV

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-dCas12a-A3A-Y130F

   Plasmid

   #193640

   Purpose

   Expression of base editor dCas12a-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescence

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   dLbCas12a-hA3A-Y130F, EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   hAPOBEC3A-Y130F

   Promoter

   CMV

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTwist-SARS-CoV-2 Δ18 B.1.351v3

   Plasmid

   #169464

   Purpose

   Encodes SARS-CoV-2 B.1.351v3 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

   Insert

   SARS-CoV-2 B.1.351v3 Spike truncated to remove 18 amino acids (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. LV_EF1a_MAPK14-P2A-Hygro_Barcode

   Plasmid

   #170239

   Purpose

   Barcoded lentiviral vector to express MAPK14 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   MAPK14 (MAPK14 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. eSpCas9-plus

   Plasmid

   #126767

   Purpose

   Expression plasmid for human codon-optimized increased fidelity eSpCas9-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   eSpCas9-plus
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   K848A, R1060A; amino acids 1005-1013 replaced wit…

   Promoter

   CBh

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOGS539_GLuc-ns

   Plasmid

   #226719

   Purpose

   Plasmid enabling yeast-mediated expression of Gaussia luciferase (GLuc)

   Depositor

   Gautam Dantas

   Article

   Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

   Insert

   Gaussia Luciferase
   

   Use

   Tags

   HA tag

   Expression

   Yeast

   Mutation

   Promoter

   pTEF1

   Available Since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGB3 alpha1 P35S:PhiC31 integrase-RDF:T35S (GB2893)

   Plasmid

   #160633

   Purpose

   TU for the constitutive expression of the PhiC31 integrase, C-terminally fused with its reversion factor (RDF)

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

   Insert

   PhiC31-RDF
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   35S

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV.hSynap-FLEX.SF-iGluSnFR.S72A

   Plasmid

   #106182

   Purpose

   Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Insert

   SF-iGluSnFR.S72A
   

   Use

   AAV

   Tags

   Expression

   Mutation

   GltI: S72A

   Promoter

   hSynapsin

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCC_02 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9NG-NLS-2A-Puro-WPRE

   Plasmid

   #139087

   Purpose

   Expresses human codon-optimized Cas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   SpCas9-NG
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   L1111R, D1135V,G1218R, E1219F, A1322R, R1335V, T1…

   Promoter

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3_SP-GCGR-V2R-NTEV-TCS-GV-2xHA

    Plasmid

    #194377

    Purpose

    Split TEV assays

    Depositor

    Michael Wehr

    Article

    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

    Insert

    SP-GCGR-V2R-NTEV-TCS-GV-2xHA (GCGR Synthetic, Human)
    

    Use

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. hCXCR4-mTFP1

    Plasmid

    #110195

    Purpose

    Encodes for human CXCR4 coding sequence tagged with the mTFP1 FP

    Depositor

    Sergi Padilla Parra

    Article

    Iliopoulou et al Nat Struct Mol Biol. 2018 Sep;25(9):814-

    Insert

    hCXCR4 (CXCR4 Human)
    

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT7-G-LbCas12a crRNA-BsaI cassette (MSP3495)

    Plasmid

    #114068

    Purpose

    T7 promoter crRNA entry vector used for in vitro transcription of LbCas12a guides

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    entry vector for LbCas12a crRNAs
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV_hsyn_NES-his-CaMPARI2-H396K-WPRE-SV40

    Plasmid

    #101062

    Purpose

    AAV vector expressing CaMPARI2_H396K (Kd = 360nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

    Depositor

    Eric Schreiter

    Article

    Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

    Insert

    CaMPARI2-H396K
    

    Use

    AAV

    Tags

    FLAG-HA-myc and NES_his

    Expression

    Mutation

    Promoter

    hsyn (synapsin-1)

    Available Since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-CARMA2sh-HA

    Plasmid

    #97072

    Purpose

    Expresses CARMA2sh/CARD14 isoform 3 in mammalian cells

    Depositor

    Pasquale Vito

    Article

    Scudiero et al J Cell Physiol. 2011 Dec;226(12):3121-31

    Insert

    CARD14 isoform 3 (CARD14 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pS6-hα2M-H6

    Plasmid

    #128495

    Purpose

    Expresses human α2M (from G27 to S1468) in mammalian cells. With C-t H6x tag.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    A2M (G27-S1468) (A2M Human)
    

    Use

    Tags

    His tag (6x)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. LentiU6-hASCL1 gRNA_multi1-3-MS2-Puro

    Plasmid

    #192682

    Purpose

    Lentiviral expression of multi gRNAs targeting hASCL1 promoter to activate human ASCL1 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Human ASCL1 activating gRNAs #1,2,3 (ASCL1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEMS2281

    Plasmid

    #111902

    Purpose

    ssAAV genome with Ple346 (NEFM MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    Ple346 (NEFM Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ple346 (NEFM MiniPromoter)

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-CoV2-Omicron BA.1 spike

    Plasmid

    #194602

    Purpose

    Mammalian expression of RBD domain from SARS-CoV-2 omicron (BA.1)

    Depositor

    Ben Murrell

    Article

    Sheward et al Lancet Infect Dis. 2022 Jun;22(6):813-82

    Insert

    CoV2-Omicron BA.1 spike (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPB-cT3G-cERP2-TSC22D3

    Plasmid

    #192916

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of TSC22D3

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    TSC22D3 (TSC22D3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEB1N-mScarlet-LZ-LOV2

    Plasmid

    #197419

    Purpose

    Mammalian expression of the mScarlet-tagged N-terminal half of photo-inactivated π-EB1

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    MAPRE1 (MAPRE1 Human)
    

    Use

    Tags

    A. sativa phototropin 1 LOV2 domain, GCN4 leucine…

    Expression

    Mammalian

    Mutation

    EB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…

    Promoter

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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