Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 11,938 results for: emb

Clear keywords
Showing: 5561 - 5580 of 11938 results

  2. pSP64-hERG1a S624A

    Plasmid
    #53056
    Purpose
    Expresses alpha subunit of S624A hERG1a K channel in Xenopus oocytes
    Depositor
    Michael Sanguinetti
    Publication
    Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
    Use
    Xenopus oocyte expression
    Mutation
    changed Serine 624 to Alanine
    Promoter
    SP6
    Available since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    53056-plasmid-map-sequence-id-82522

    Enlarge

  5. pGL-p21UTRm2

    Plasmid
    #20879
    Depositor
    Robert Blelloch
    Publication
    Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    p21 3'UTR site 2 mutation (Cdkn1a Mouse)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    Predicted miRNA binding site 2 (Position ~1100) G…
    Available since
    March 12, 2012
    Availability
    Academic Institutions and Nonprofits only

  10. CCR5-SZ190b-sfGFP

    Plasmid
    #162447
    Purpose
    Expression in HEK293T cell and compete ligand singaling against full-length receptors, the truncated receptor can perform signaling at high ligand concentration
    Depositor
    Shuguang Zhang
    Publication
    Qing et al iScience. 2020 Oct 28;23(12):101670. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    C-C chemokine receptor type 5 (CCR5 Human)
    Expression
    Mammalian
    Mutation
    truncation from aa88 to aa249
    Promoter
    CMV
    Available since
    Aug. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    162447-plasmid-map-sequence-id-331353

    Enlarge

  11. pCRI018-pKW20088-PelcA-mCherry-TtrpC-PU3-E1-Cas9Scaffold-8xT

    Plasmid
    #140205
    Purpose
    Proof-of-concept of CRISPR/dSpCas9-VPR activation, encoding PelcA-mCherry along with the sgRNA E1 expression cassette, targeting PelcA.
    Depositor
    Yit Heng Chooi
    Publication
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    mCherry
    sgRNA E1
    Use
    CRISPR and Synthetic Biology; Proof-of-concept te…
    Mutation
    G174D
    Promoter
    PelcA and U3
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    140205-plasmid-map-sequence-id-310180

    Enlarge

  12. pelB-MBP-HtrA-ss

    Plasmid
    #137066
    Purpose
    E. coli expression clone (T7lac promoter) for mature HtrA (aa 28-474) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage
    Depositor
    Debra Hansen
    Publication
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66500.2 (BB_0104 Borrelia burgdorferi B31)
    Tags
    PelB signal peptide + His10 + maltose binding pro…
    Expression
    Bacterial
    Mutation
    mature HtrA: lacks the HtrA signal peptide
    Promoter
    T7lac
    Available since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137066-plasmid-map-sequence-id-291956

    Enlarge

  15. GFP-CRY2-P14P5K

    Plasmid
    #79569
    Purpose
    expression of GFP-tagged CRY2PHR fused to iSH2 domain of mouse Pip5k1c
    Depositor
    Olof Idevall-Hagren
    Publication
    Xie et al Cell Chem Biol. 2016 Jul 21;23(7):816-26
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Pip5k1c (Pip5k1c Mouse)
    Tags
    CRY2 (photolyase homology region domain of Arabid…
    Expression
    Mammalian
    Mutation
    R445E and R446E; truncation of amino acids 636-66…
    Promoter
    CMV
    Available since
    Aug. 9, 2016
    Availability
    Academic Institutions and Nonprofits only

  16. pN1 VAMP2 R125TAG

    Plasmid
    #69877
    Purpose
    express in mammalian cells for unnatural amino acid incorporation into VAMP2
    Depositor
    Silvio Rizzoli
    Publication
    Vreja et al ACS Nano. 2015 Nov 24;9(11):11034-41. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    VAMP2 (Vamp2 Rat)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    Amber and Ochre stop codons in the former linker …
    Available since
    Jan. 25, 2016
    Availability
    Academic Institutions and Nonprofits only

  20. pETM11-SUMO3-KSR1-CA3-sfGFP (C-KSR)

    Plasmid
    #217766
    Purpose
    Fluorescent reporter for ceramide (for bacterial expression and purification)
    Depositor
    Paula Nunes-Hasler
    Publication
    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do
    Insert
    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    Use
    Protein purification (6xhis tag, tev site, sumo3 …
    Tags
    sfGFP
    Expression
    Bacterial
    Mutation
    CA3 domain aa 317-400 plus vector-based linker LE…
    Promoter
    T7 promoter
    Available since
    June 10, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    217766-plasmid-map-sequence-id-435787

    Enlarge

Showing: 5561 - 5580 of 11938 results