Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 11,701 results for: phen

Showing: 1351 - 1400 of 11701 results

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PCR4-Shh::lacZ-H11

Plasmid

#139098

Purpose

enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Shh minimal promoter)

Depositor

Len Pennacchio

Article

Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

Type

Empty backbone

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

Shh

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pMDC32B-AtTAS1c-D2-B/c-AtMIR173

Plasmid

#137885

Purpose

Plant expression vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor downstream 3'D1[+]. Contains AtMIR173 for syntasi expression in any plant species.

Depositor

Alberto Carbonell

Article

Lopez-Dolz et al Nucleic Acids Res. 2020 May 12. pii: 583

Inserts

AtTAS1c-D2-B/c
2x35S-AtMIR173-Tnos

Expression

Plant

Mutation

A. thaliana TAS1c precursor sequence including a …

Available Since

May 14, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) K-Ras(G12C)

Plasmid

#224284

Purpose

Cell transfection and expression of K-Ras (G12C)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

K-Ras (KRAS Human)

Expression

Mammalian

Mutation

G12C

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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PCR4-Hsp68::lacZ-H11

Plasmid

#139099

Purpose

enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Hsp68 minimal promoter)

Depositor

Len Pennacchio

Article

Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

Type

Empty backbone

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

Hsp68

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+) K-Ras(WT)

Plasmid

#224283

Purpose

Cell transfection and expression of K-Ras

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

K-Ras (KRAS Human)

Expression

Mammalian

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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p_sc-eVIP

Plasmid

#168174

Purpose

Expresses barcoded coding genetic variants in mammalian cells

Depositor

Jesse Boehm, James T. Neal, Aviv Regev

Article

Ursu et al Nat Biotechnol. 2022 Jun;40(6):896-905.

Insert

TP53 (TP53 Human)

Use

Lentiviral

Tags

N/A

Expression

Mammalian

Mutation

Wildtype

Promoter

EF-1a

Available Since

Jan. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pBABE-hygro-2xMyc-Rac1-P29S

Plasmid

#128581

Purpose

For mammalian cell expression of P29S mutant murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.

Depositor

Julian Downward

Article

Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

Insert

Rac1 (Rac1 Mouse)

Use

Retroviral

Tags

2xMyc epitope

Expression

Mammalian

Mutation

Changed Proline29 to Serine.

Available Since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pBABE-hygro-2xMyc-Rac1-Q61L

Plasmid

#128582

Purpose

For mammalian cell expression of Q61L mutant murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.

Depositor

Julian Downward

Article

Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

Insert

Rac1 (Rac1 Mouse)

Use

Retroviral

Tags

2xMyc epitope

Expression

Mammalian

Mutation

Changed Glutamine61 to Leucine.

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_STC2_WT

Plasmid

#82883

Purpose

Gateway Donor vector containing STC2, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

STC2 (STC2 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpdCas9-huTET1CD-T2A-GFP (PX458)

Plasmid

#129025

Purpose

targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-EGFP and cloning backbone for sgRNA

Depositor

Julia K Polansky

Article

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-huTET1CD, SgRNA cloning site

Tags

HA-Tag, NLS and T2A-EGFP

Expression

Mammalian

Mutation

dCas9 (D10A;H840A)

Promoter

CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-hSNCA

Plasmid

#170445

Purpose

Lentiviral vector expressing human SNCA, under control of EF1alpha promoter.

Depositor

Sang-Hun Lee

Article

Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

Insert

human SNCA (SNCA Human)

Use

Lentiviral

Promoter

EF1a

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-ARAP3

Plasmid

#39484

Depositor

Sonja Vermeren

Article

Krugmann et al Mol Cell. 2002 Jan;9(1):95-108.

Insert

ARAP3 (ARAP3 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 22, 2012

Availability

Academic Institutions and Nonprofits only
pSpdCas9-huTET1CD-T2A-mCherry(PX458)

Plasmid

#129027

Purpose

targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-mCherry and cloning backbone for sgRNA

Depositor

Julia K Polansky

Article

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-huTET1CD, SgRNA cloning site

Tags

HA-Tag, NLS and T2A-mCherry

Expression

Mammalian

Mutation

dCas9 (D10A;H840A)

Promoter

CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pSpdCas9-hudTET1CD-T2A-GFP (PX458)

Plasmid

#129026

Purpose

inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-EGFP and cloning backbone for sgRNA

Depositor

Julia K Polansky

Article

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-hudTET1CD, SgRNA cloning site

Tags

HA-Tag, NLS and T2A-EGFP

Expression

Mammalian

Mutation

dCas9 (D10A;H840A), catalytic domain huTET1 inact…

Promoter

CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_S100A13_WT_V5

Plasmid

#82975

Purpose

Gateway Donor vector containing S100A13, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

S100A13 (S100A13 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_PYGL_WT_V5

Plasmid

#82991

Purpose

Gateway Donor vector containing PYGL, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

PYGL (PYGL Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-FRT/T0-Flag-BRIP1

Plasmid

#222619

Purpose

Mammalian expression vector of flag-tagged BRIP1

Depositor

Daniel Durocher

Article

Zhao et al Mol Cell. 2023 Aug 3;83(15):2792-2809.e9

Insert

BRIP1 (BRIP1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONR223_RIT1_WT

Plasmid

#82882

Purpose

Gateway Donor vector containing RIT1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RIT1 (RIT1 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_ZBTB24_WT

Plasmid

#82887

Purpose

Gateway Donor vector containing ZBTB24, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

ZBTB24 (ZBTB24 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_STK11_p.D176H

Plasmid

#82870

Purpose

Gateway Donor vector containing STK11, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

STK11 (STK11 Human)

Use

Gateway entry vector

Mutation

D176H

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_STK11_p.G251F

Plasmid

#82862

Purpose

Gateway Donor vector containing STK11, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

STK11 (STK11 Human)

Use

Gateway entry vector

Mutation

G251F

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_STK11_p.G56V

Plasmid

#82760

Purpose

Gateway Donor vector containing STK11, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

STK11 (STK11 Human)

Use

Gateway entry vector

Mutation

G56V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_STK11_p.G242W

Plasmid

#82763

Purpose

Gateway Donor vector containing STK11, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

STK11 (STK11 Human)

Use

Gateway entry vector

Mutation

G242W

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_STK11_p.G251V

Plasmid

#82765

Purpose

Gateway Donor vector containing STK11, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

STK11 (STK11 Human)

Use

Gateway entry vector

Mutation

G251V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_STK11_p.L245F

Plasmid

#82751

Purpose

Gateway Donor vector containing STK11, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

STK11 (STK11 Human)

Use

Gateway entry vector

Mutation

L245F

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_NUP88_WT_V5

Plasmid

#83011

Purpose

Gateway Donor vector containing NUP88, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

NUP88 (NUP88 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_AKR1B1_WT

Plasmid

#82928

Purpose

Gateway Donor vector containing AKR1B1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

AKR1B1 (AKR1B1 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
EIF2B3

Plasmid

#172072

Purpose

In vitro transcription for rescue experiments

Depositor

Cheol-Hee Kim

Article

Lee et al Hum Mol Genet. 2021 Apr 27;30(5):331-342

Insert

EIF2B3 (EIF2B3 Human)

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

July 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpdCas9-hudTET1CD-T2A-mCherry(PX458)

Plasmid

#129028

Purpose

inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-mCherry and cloning backbone for sgRNA

Depositor

Julia K Polansky

Article

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-hudTET1CD, SgRNA cloning site

Tags

HA-Tag, NLS and T2A-mCherry

Expression

Mammalian

Mutation

dCas9 (D10A;H840A), catalytic domain huTET1 inact…

Promoter

CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_PCCB_WT_V5

Plasmid

#82938

Purpose

Gateway Donor vector containing PCCB, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

PCCB (PCCB Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_POLR2I_WT_V5

Plasmid

#82955

Purpose

Gateway Donor vector containing POLR2I, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

POLR2I (POLR2I Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_SMAD4_p.P356R

Plasmid

#82769

Purpose

Gateway Donor vector containing SMAD4, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

SMAD4 (SMAD4 Human)

Use

Gateway entry vector

Mutation

P356R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_RBM45_WT_V5

Plasmid

#82968

Purpose

Gateway Donor vector containing RBM45, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RBM45 (RBM45 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CAG-NLS-DNMT3A-L-N-spC9-N-intein-hGH

Plasmid

#112212

Purpose

Targeted DNA methylation

Depositor

Xingxu Huang

Article

Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

Insert

N-terminus of dCas9 and DNMT3A (DNMT3A Human)

Use

AAV

Mutation

H840A,N863A

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_STC2_WT_V5

Plasmid

#82957

Purpose

Gateway Donor vector containing STC2, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

STC2 (STC2 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_NOSIP_WT_V5

Plasmid

#82970

Purpose

Gateway Donor vector containing NOSIP, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

NOSIP (NOSIP Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_PSMD9_WT_V5

Plasmid

#82950

Purpose

Gateway Donor vector containing PSMD9, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

PSMD9 (PSMD9 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_BRP44_WT_V5

Plasmid

#82976

Purpose

Gateway Donor vector containing BRP44, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

MPC2 (MPC2 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-Enhancer-ZC3H12D_PromoterMUT

Plasmid

#153068

Purpose

ZC3H12D promoter region carrying mutations in BHLHE40 binding sites, cloned upstream of luciferase reporter

Depositor

Silvia Monticelli

Article

Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

Insert

ZC3H12D promoter region mutated in BHLHE40 binding sites (ZC3H12D Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutagenesis to disrupt 3 BHLHE40 binding sites. N…

Promoter

None

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-8

Plasmid

#129036

Purpose

targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA8 targeting human TSDR

Depositor

Julia K Polansky

Article

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-huTET1CD, SgRNA8 (huTSDR)

Tags

HA-Tag, NLS and T2A-mCherry

Expression

Mammalian

Mutation

dCas9 (D10A;H840A)

Promoter

CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_RBM45_p.D434Y

Plasmid

#82758

Purpose

Gateway Donor vector containing RBM45 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RBM45 (RBM45 Human)

Use

Gateway entry vector

Mutation

D434Y

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_RBM45_p.M126I

Plasmid

#82759

Purpose

Gateway Donor vector containing RBM45 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RBM45 (RBM45 Human)

Use

Gateway entry vector

Mutation

M126I

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-1

Plasmid

#129053

Purpose

targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA1 targeting mouse TSDR

Depositor

Julia K Polansky

Article

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-huTET1CD, SgRNA1 (mouseTSDR)

Tags

HA-Tag, NLS and T2A-mCherry

Expression

Mammalian

Mutation

dCas9 (D10A;H840A)

Promoter

CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-1

Plasmid

#129029

Purpose

targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA1 targeting human TSDR

Depositor

Julia K Polansky

Article

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-huTET1CD, SgRNA1 (huTSDR)

Tags

HA-Tag, NLS and T2A-mCherry

Expression

Mammalian

Mutation

dCas9 (D10A;H840A)

Promoter

CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_RBKS_WT_V5

Plasmid

#82959

Purpose

Gateway Donor vector containing RBKS, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RBKS (RBKS Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_STAP2_WT_V5

Plasmid

#83000

Purpose

Gateway Donor vector containing STAP2, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

STAP2 (STAP2 Human)

Use

Gateway entry vector

Mutation

D93N

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_RIT1_p.F82L

Plasmid

#82739

Purpose

Gateway Donor vector containing RIT1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RIT1 (RIT1 Human)

Use

Gateway entry vector

Mutation

F82L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SMAD4_p.P215S

Plasmid

#82829

Purpose

Gateway Donor vector containing SMAD4, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

SMAD4 (SMAD4 Human)

Use

Gateway entry vector

Mutation

P215S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SMAD4_p.D493N

Plasmid

#82788

Purpose

Gateway Donor vector containing SMAD4, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

SMAD4 (SMAD4 Human)

Use

Gateway entry vector

Mutation

D493N

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SMAD4_p.V492F

Plasmid

#82785

Purpose

Gateway Donor vector containing SMAD4, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

SMAD4 (SMAD4 Human)

Use

Gateway entry vector

Mutation

V492F

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

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