Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 2,347 results for: puc19

Showing: 1351 - 1400 of 2347 results

  4. pC1.530

    Plasmid
    #205441
    Purpose
    Level 1 Position 1 pUC19 vector with sgRNA and GmR. Used for homologous recombination with Cas12a editing vector pC1.509. sgRNA targets pC1.509 for removal.
    Depositor
    Alistair McCormick
    Article
    Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:
    Insert
    RepB gRNA
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    May 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    205441-plasmid-map-sequence-id-408759

    Enlarge

  10. pADH139

    Plasmid
    #90987
    Purpose
    NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR; use with pADH110 to generate target-specific part 2 of 2 for C.mal LEUpOUT CRISPR system.
    Depositor
    Aaron Hernday
    Article
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Insert
    gRNA conserved, C, mal LEU2 2 of 2
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    90987-plasmid-map-sequence-id-180520

    Enlarge

  24. pIF251

    Plasmid
    #133935
    Purpose
    Kanamycin selection marker flanked by ~200 bp of homology to bacteriophage lambda. The selection marker will recombineer 21.3-22.8 kb away from the cosL DNA end of the phage genome.
    Depositor
    IIya Finkelstein
    Article
    Kim et al Sci Rep. 2017 May 18;7(1):2071. doi: 10.
    Insert
    Kanamycin selection marker flanked by ~200 bp of homology to bacteriophage lambda
    Use
    Λ-phage recombineering plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    March 3, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    133935-plasmid-map-sequence-id-269109

    Enlarge

  26. pILGFP3

    Plasmid
    #65451
    Purpose
    pILGFP3 was designed to test the promoter activity using the yeast enhanced green fluorescent protein (yEGFP) as reporter.
    Depositor
    Claudia Vickers
    Article
    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi
    Type
    Empty backbone
    Use
    Tags
    yEGFP reporter
    Expression
    Yeast
    Mutation
    Promoter
    Available Since
    June 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    65451-plasmid-map-sequence-id-110968

    Enlarge

  27. pITGFP3

    Plasmid
    #65452
    Purpose
    pITGFP3 was designed to test the promoter activity using the destabilized yeast enhanced green fluorescent protein (yEGFP-CLN2PEST) as reporter.
    Depositor
    Claudia Vickers
    Article
    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi
    Type
    Empty backbone
    Use
    Tags
    yEGFP-CLN2PEST reporter
    Expression
    Yeast
    Mutation
    Promoter
    Available Since
    June 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  34. pJAID5VY

    Plasmid
    #165048
    Purpose
    Integrative expression of CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.
    Depositor
    Claudia Vickers
    Article
    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:
    Insert
    P-URA3>KlURA3>T-AgTEF1-P-TEF1>CUP1-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
    Use
    Tags
    Expression
    Yeast
    Mutation
    OsTIR1 is codon-optimised for expression in S. ce…
    Promoter
    Available Since
    March 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    165048-plasmid-map-sequence-id-327148

    Enlarge

  35. pJAID8Z104

    Plasmid
    #165056
    Purpose
    Integrative expression of yEGFP-AID*-CUP1 and SKP1-fusing rice auxin receptor gene OsTIR1.
    Depositor
    Claudia Vickers
    Article
    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:
    Insert
    P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>T-PGK1-P-ACS2> SKP1>OsTIR1opt>T-URA3
    Use
    Tags
    Expression
    Yeast
    Mutation
    WT
    Promoter
    Available Since
    March 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    165056-plasmid-map-sequence-id-327155

    Enlarge

  45. pDM043

    Plasmid
    #216813
    Purpose
    Aspergillus nidulans codon-adjusted mScarlet3 fluorescent protein, includes linker for N-terminal tagging.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Insert
    mScarlet3
    Use
    Tags
    VinnyLinker
    Expression
    Bacterial
    Mutation
    Aspergillus nidulans codon-adjusted
    Promoter
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216813-plasmid-map-sequence-id-430146

    Enlarge

  46. pDM052

    Plasmid
    #216814
    Purpose
    Aspergillus nidulans codon-adjusted mNeonGreen fluorescent protein, includes linker for C-terminal tagging.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Insert
    mNeonGreen
    Use
    Tags
    VinnyLinker
    Expression
    Bacterial
    Mutation
    Aspergillus nidulans codon-adjusted
    Promoter
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216814-plasmid-map-sequence-id-429539

    Enlarge

  47. pDM070

    Plasmid
    #216816
    Purpose
    Aspergillus nidulans codon-adjusted mCardinal fluorescent protein, includes linker for C-terminal tagging.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Insert
    mCardinal
    Use
    Tags
    VinnyLinker
    Expression
    Bacterial
    Mutation
    Aspergillus nidulans codon-adjusted
    Promoter
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216816-plasmid-map-sequence-id-429610

    Enlarge

Showing: 1351 - 1400 of 2347 results