We narrowed to 1,940 results for: 974

1. T7opt in pCAGGS

   Plasmid

   #65974

   Purpose

   Human codon-optimized T7 polymerase

   Depositor

   Benhur Lee

   Article

   Yun et al J Virol. 2015 Jan 15;89(2):1242-53. doi:

   Insert

   T7-opt
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. YopH (residues 164-468)

   Plasmid

   #79749

   Purpose

   This plasmid encodes the active catalytic domain (amino acid residues 164-468) of YopH. Inteded for co-expression with human Tyr kinase plasmids to enhance bacterial kinase expression.

   Depositor

   Nicholas Levinson, John Chodera, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   YopH (residues 164-468)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV SYN PSAM4 GlyR IRES EGFP

   Plasmid

   #119742

   Purpose

   Chemogenetic inhibitor expression plasmid

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2019 Mar 14. pii: science.aav52

   Has Service

   AAV5

   Insert

   PSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   L131G, Q139L, Y217F

   Promoter

   synapsin

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMSCVpuro-DEST

   Plasmid

   #119745

   Purpose

   Allows Gateway cloning of gene of interest into retroviral pMSCV vector (with Puromycin resistance)

   Depositor

   Andrew Jackson, Martin Reijns

   Article

   Benitez-Guijarro et al EMBO J. 2018 Aug 1;37(15). pii: embj.201

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV SYN flex PSAM4 GlyR IRES EGFP

   Plasmid

   #119741

   Purpose

   Cre-dependent chemogenetic inhibitor expression

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2019 Mar 14. pii: science.aav52

   Has Service

   AAV5 and AAV9

   Insert

   PSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   L131G, Q139L, Y217F

   Promoter

   synapsin

   Available since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pITR-AAV-Rep2Cap9-GFP

   Plasmid

   #199744

   Purpose

   Expresses AAV2 Rep and AAV9 Cap, with GFP replacing a region of Cap, within AAV2 ITRs.

   Depositor

   Aravind Asokan

   Article

   Gonzalez et al Nat Protoc. 2023 Sep 21. doi: 10.1038/s4

   Insert

   AAV9 Cap w/ GFP insert
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Replaced amino acids 356-736 in Cap with GFP

   Promoter

   Available since

   Oct. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-hnRNPU-V5

   Plasmid

   #35974

   Depositor

   Susana Valente

   Article

   Mediouni et al Curr HIV Res. 2015;13(1):64-79.

   Insert

   heterogeneous nuclear ribonucleoprotein U (HNRNPU Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFORF1752

   Plasmid

   #143974

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZNF180 (ZNF180 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Flag- MCL1-3A (S159, S162, T163 mutated to A)

   Plasmid

   #197458

   Purpose

   MCL-1 phosphodegron mutants

   Depositor

   Omar Khan

   Article

   Keyan et al Cancers (Basel). 2023 Jan 13;15(2):505.

   Insert

   MCL1 (MCL1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S159A, S161A, T163A

   Promoter

   CMV

   Available since

   Aug. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX FLAG-SLC38A9.1

    Plasmid

    #199749

    Purpose

    Lentiviral construct to stably express SLC38A91.1

    Depositor

    Roberto Zoncu

    Article

    Shin et al Science. 2022 Sep 16;377(6612):1290-1298

    Insert

    SLC38A9.1
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV CAMKII PSAM4 GlyR IRES EGFP

    Plasmid

    #119744

    Purpose

    Chemogenetic inhibitor expression plasmid

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2019 Mar 14. pii: science.aav52

    Has Service

    AAV5

    Insert

    PSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    L131G, Q139L, Y217F

    Promoter

    CamKII

    Available since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pY003 (pFnCpf1_delta Cas)

    Plasmid

    #69974

    Purpose

    Expresses FnCpf1 and spacers 1-4 of CRISPR array. Cas genes are removed.

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    FnCpf1 locus without Cas genes
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Cas genes are deleted from locus

    Promoter

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEND-304_Cacnes_tetR

    Plasmid

    #225619

    Purpose

    C. acnes with tetR-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+TetR // P(BBa_23119-TetO1O3)+RBS_1+mCherry

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Insert

    TetR
    

    Use

    Synthetic Biology

    Tags

    6xHis and mCherry-V5-6xHis

    Expression

    Bacterial

    Mutation

    Codon optimized for Cutibacterium acnes

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEND-375_Cacnes_phlF

    Plasmid

    #225620

    Purpose

    C. acnes with PhlF-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+PhlF // P(BBa_23119-PhlO1O3)+RBS_1+mCherry.

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Insert

    PhlF
    

    Use

    Synthetic Biology

    Tags

    6xHis and mCherry-V5-6xHis

    Expression

    Bacterial

    Mutation

    Codon optimized for Cutibacterium acnes

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. Nrp2.3-AP-His

    Plasmid

    #71974

    Purpose

    Expresses the extracellular region of the Neuropilin 2, isoform 3 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Nrp2.3 (Nrp2 Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. FAK2_HUMAN_D0

    Plasmid

    #79747

    Purpose

    This plasmid encodes the kinase domain of FAK2. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    FAK2 (PTK2B Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

17. Orco-T2A-QF2-9xQUAS-GCaMP6f-3XP3-dsRed

    Plasmid

    #157974

    Purpose

    Template plasmid for inserting GCaMP reporter into the Orco gene of Aedes aegypti with CRISPR/Cas9

    Depositor

    Carolyn McBride

    Article

    Zhao et al Nature. 2022 May;605(7911):706-712. doi:

    Insert

    Orco
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. AICSDP-55:DMD-mEGFP

    Plasmid

    #159744

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human DMD via Cas9-excisable CAGGS-mCherry selection cassette

    Depositor

    Allen Institute for Cell Science

    Insert

    DMD Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (DMD Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. TFORF2685

    Plasmid

    #142974

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZMYND11 (ZMYND11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. STK24_HUMAN_D0

    Plasmid

    #79742

    Purpose

    This plasmid encodes the kinase domain of STK24. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    STK24 (STK24 Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only