Addgene

We narrowed to 1,088 results for: Streptococcus

Showing: 541 - 560 of 1088 results

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pBBK01 pCAS-Tyr-[gRNA: BsaI GFP dropout] (KanR, AmpR)

Plasmid

#178985

Purpose

Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Kanamycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pspCas9-3'UTR-Tetra-com-vector

Plasmid

#132553

Purpose

Expresses spCas9 and sgRNA scaffold with com aptamer replacing the Tetraloop

Depositor

Baisong Lu

Article

Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

Inserts

Cas9
sgRNA scaffold with com replacing the Tetraloop

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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nos-Cas9.874Z1

Plasmid

#112685

Purpose

Express hSpCas9 under nanos promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

hSpCas9

Use

Tags

3x FLAG, EGFP, and NLS

Expression

Insect

Mutation

Humanized Cas9 bacterial sequence

Promoter

nanos

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pBBK05 pCAS-Tyr-[gRNA: BsaI GFP dropout] (NatR,AmpR)

Plasmid

#178989

Purpose

Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Nourseothricin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pJT25_GalL_BE3

Plasmid

#143736

Purpose

Expresses BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

BE3

Use

CRISPR

Tags

Expression

Yeast

Mutation

spCas9(D10A)

Promoter

GalL

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST-Cas9-tdTomato

Plasmid

#141013

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

Cas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pIntegrase_3

Plasmid

#60575

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_3

Use

Tags

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pIntegrase_10

Plasmid

#60581

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_10

Use

Tags

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available since

March 5, 2015

Availability

Academic Institutions and Nonprofits only

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pIntegrase_4

Plasmid

#60576

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_4

Use

Tags

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCF730

Plasmid

#121668

Purpose

EF1a-ProCas9-Flavi-P2A-Puro

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Insert

Cas9(C)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF-1a core promoter

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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Sox1/2/3-2373/+3>nls::Cas9::nls

Plasmid

#59989

Purpose

Sox1/2/3 (SoxB1) driver driving nls::Cas9::nls

Depositor

Lionel Christiaen

Article

Stolfi et al Development. 2014 Nov;141(21):4115-20. d

Insert

nls::Cas9::nls

Use

CRISPR

Tags

Expression

Mutation

Reverted mutations from dCas9 to wiltdtype

Promoter

Ciinte.Sox1/2/3 (SoxB1) -2373/+3

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pJT42_GalL_BE-PAPAPA

Plasmid

#145035

Purpose

Expresses BE-PAPAPA in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

BE-PAPAP

Use

CRISPR

Tags

Expression

Yeast

Mutation

spCas9(D10A)

Promoter

GalL

Available since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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pHCas9-Nours

Plasmid

#107733

Purpose

Escherichia coli- S. cerevisiae shuttle plasmid harbors a Cas9 gene, a natMX6 and URA3 selection markers used in S. cerevisiae

Depositor

Sheng Yang

Article

Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.

Insert

Cas9

Use

CRISPR

Tags

SV40 NLS

Expression

Yeast

Mutation

Human optimized

Promoter

TEF1

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pRS315e_pGal-dCas9-SH3

Plasmid

#79613

Purpose

Expresses dCas9-SH3 in yeast cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Use

Tags

SH3 domain

Expression

Yeast

Mutation

D10A and H840A for nuclease deficient Cas9

Promoter

pGal1

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pCF204

Plasmid

#121656

Purpose

U6-sgRNA EFS-Cas9-wt-P2A-Puro

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Insert

Cas9

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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LacD_WT_pET21a

Plasmid

#29785

Depositor

SeWon Suh

Article

Lee et al FEBS Lett. 2011 Jan 21. 585(2):307-12.

Insert

Tagatose 1,6-diphosphate aldolase

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

July 7, 2011

Availability

Academic Institutions and Nonprofits only
LacD_L165E_pET21a

Plasmid

#29786

Depositor

SeWon Suh

Article

Lee et al FEBS Lett. 2011 Jan 21. 585(2):307-12.

Insert

Tagatose 1,6-diphosphate aldolase

Use

Tags

His

Expression

Bacterial

Mutation

L165E

Promoter

Available since

July 7, 2011

Availability

Academic Institutions and Nonprofits only
LacD_L275S_pET21a

Plasmid

#29787

Depositor

SeWon Suh

Article

Lee et al FEBS Lett. 2011 Jan 21. 585(2):307-12.

Insert

Tagatose 1,6-diphosphate aldolase

Use

Tags

His

Expression

Bacterial

Mutation

L275S

Promoter

Available since

July 7, 2011

Availability

Academic Institutions and Nonprofits only
LacD_L165E/L275S_pET21a

Plasmid

#29788

Depositor

SeWon Suh

Article

Lee et al FEBS Lett. 2011 Jan 21. 585(2):307-12.

Insert

Tagatose 1,6-diphosphate aldolase

Use

Tags

His

Expression

Bacterial

Mutation

L165E and L275S

Promoter

Available since

July 7, 2011

Availability

Academic Institutions and Nonprofits only
pENTR-L3-Cas9-L2

Plasmid

#141038

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

Cas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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