Addgene

We narrowed to 8,125 results for: gnal

Showing: 541 - 560 of 8125 results

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pLenti-FoxO1-Clover

Plasmid

#67759

Purpose

Fluorescent fusion protein for tracking Akt signaling

Depositor

Peter Rotwein

Article

Gross et al J Cell Sci. 2015 Jun 3. pii: jcs.168773.

Insert

Fox01 (Foxo1 Mouse)

Use

Lentiviral

Tags

Clover

Expression

Mutation

Changed Serine 209 to Alanine, changed Histidine …

Promoter

EF1a

Available since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pGL3-NFAT luciferase

Plasmid

#17870

Purpose

3x NFAT binding sequence upstream of a luciferase reporter; can be used to assay signal transduction through Ca2+, calcineurin and NFAT in any tissue

Depositor

Jerry Crabtree

Article

Clipstone et al Nature. 1992 Jun 25. 357(6380):695-7.

Insert

3x NFAT binding sequence (IL2 )

Use

Luciferase

Tags

IL2 promoter and Luciferase

Expression

Mammalian

Mutation

Promoter

IL-2 promoter

Available since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

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pRAB18-GFP

Plasmid

#106984

Purpose

ABA signaling reporter, GFP under the RAB18 promoter

Depositor

Julian Schroeder

Article

Kim et al Curr Biol. 2011 Jun 7;21(11):990-7. doi:

Insert

sGFP

Use

Tags

Expression

Plant

Mutation

Promoter

RAB18

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-cytoEKAR4

Plasmid

#174438

Purpose

Cytosol-targeted EKAR4.

Depositor

Jin Zhang

Article

Keyes et al eLife. 2020 May 26;9. pii: 57410. doi: 1

Insert

cytoEKAR4

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pDN77

Plasmid

#61347

Purpose

mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/IkBα NES)

Depositor

Roland Eils, Barbara Di Ventura

Article

Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

Insert

mCherry

Use

Tags

GS linker-AsLov2-biNLS2 and NES IkBa

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-nuclear-EKAR4

Plasmid

#174439

Purpose

Nuclear-targeted EKAR4.

Depositor

Jin Zhang

Article

Keyes et al eLife. 2020 May 26;9. pii: 57410. doi: 1

Insert

nuclear-EKAR4

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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7Gli:GFP

Plasmid

#110494

Purpose

reporter of Hedgehog signaling activity

Depositor

Michael Lewis

Article

Li et al PLoS Genet. 2016 May 19;12(5):e1006054.

Insert

7 repeats of the Gli binding site GAACACCCA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

7 repeats of Gli biding site

Available since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST-V1-ORF

Plasmid

#73635

Purpose

Gateway™ destination vector encoding N-terminal fusion with Venus fluorescent protein fragment 1 (V1).

Depositor

Darren Saunders

Article

Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

Type

Empty backbone

Use

Tags

Venus fragment 1 (V1)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST-V2-ORF

Plasmid

#73636

Purpose

Gateway™ destination vector encoding N-terminal fusion with Venus fluorescent protein fragment 2 (V2).

Depositor

Darren Saunders

Article

Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

Type

Empty backbone

Use

Tags

Venus fragment 2 (V2)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2016

Availability

Academic Institutions and Nonprofits only

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pDN34

Plasmid

#61343

Purpose

mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/PKIt NES)

Depositor

Roland Eils, Barbara Di Ventura

Article

Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

Insert

mCherry

Use

Tags

GS linker-AsLov2-biNLS2 and PKIt NES

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pAL175

Plasmid

#22276

Purpose

Expresses YFP-PIF for red-light-induced binding to Phy

Depositor

Christopher Voigt

Article

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

PIF6APB (PIL2 Mustard Weed)

Use

Synthetic Biology

Tags

mYFP

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pFastBac1-Ls-AChBP

Plasmid

#63126

Purpose

Expression of Lymnaea stagnalis AChBP

Depositor

Titia Sixma

Article

Celie et al Nat Struct Mol Biol. 2005 Jul;12(7):582-

Insert

AChBP

Use

Tags

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Nuc-ExRai-AMPKAR

Plasmid

#192450

Purpose

ExRai-AMPKAR targeted to the nucleus.

Depositor

Jin Zhang

Article

Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

Insert

ExRai-AMPKAR-NLS

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pDB22

Plasmid

#61342

Purpose

Mammalian expression of mCherry fused to LINuS, a light-inducible nuclear localization signal (cMycP1A NLS/PKIt NES)

Depositor

Roland Eils, Barbara Di Ventura

Article

Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

Insert

mCherry

Use

Tags

GS linker-AsLov2-cMyc1A NLS and PKIt NES

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pCW57.1-ZNF746

Plasmid

#207825

Purpose

Dox-inducible expression of ZNF746

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

ZNF746 (ZNF746 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

TRE

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pDN42

Plasmid

#61345

Purpose

mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS10/PKIt NES)

Depositor

Roland Eils, Barbara Di Ventura

Article

Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

Insert

mCherry

Use

Tags

GS linker-AsLov2-biNLS10 and PKIt NES

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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FLAG-mFzd4-ΔC(40-513)-3C-sGFP1-10_pVL1393

Plasmid

#216380

Purpose

Baculovirus transfer vector to express FLAG-tagged mouse Fzd4 ΔC (residues 40-513) fused to split GFP (strands 1-10)

Depositor

William Weis

Article

Bruguera et al bioRxiv 2024.02.03.578714

Insert

Frizzled-4 (Fzd4 Mouse)

Use

Baculovirus

Tags

3C-splitGFP1to10opt-ETVV and HA signal sequence-F…

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAL188

Plasmid

#22277

Purpose

Expresses Tiam DH-PH domains fused to mYFP-PIF6, for optogenetic recruitment

Depositor

Christopher Voigt

Article

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

TiamDHPH-20aaLinker-mYFP-PIF6APB (PIL2 Mustard Weed)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pCDNA3 T7 Jip1

Plasmid

#51699

Purpose

JIP-1 may suppress signal transduction by the JNK pathway"

Depositor

Roger Davis

Article

Dickens et al Science. 1997 Aug 1. 277(5326):693-6.

Insert

Jnk interacting protein 1 (Mapk8ip1 Mouse)

Use

Tags

T7

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)-ExRai-AKAR-NES

Plasmid

#118407

Purpose

Cytosol-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

ExRai-AKAR-NES

Use

Tags

Nuclear export signal (NES)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

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