We narrowed to 20,861 results for: OMP

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pHH0103_SH3GL1-1/1

Plasmid

#91473

Purpose

Protein expression and purification of human SH3 domain construct SH3GL1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3GL1-1/1 (SH3GL1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_STAC3-1/2

Plasmid

#91271

Purpose

Protein expression and purification of human SH3 domain construct STAC3-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

STAC3-1/2 (STAC3 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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Flag-ATXN1_V591A_S602D

Plasmid

#48190

Purpose

Expresses V591A and S602D mutated Ataxin1

Depositor

Huda Zoghbi

Article

Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

Insert

Ataxin1 (ATXN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2013

Availability

Academic Institutions and Nonprofits only

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Flag-ATXN1_V591A_L686E

Plasmid

#48191

Purpose

Expresses V591A and L686E mutated Ataxin1

Depositor

Huda Zoghbi

Article

Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

Insert

Ataxin1 (ATXN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2013

Availability

Academic Institutions and Nonprofits only

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Flag-ATXN1_S602D_L686E

Plasmid

#48192

Purpose

Expresses S602D and L686E mutated Ataxin1

Depositor

Huda Zoghbi

Article

Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

Insert

Ataxin1 (ATXN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2013

Availability

Academic Institutions and Nonprofits only

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pKT3Ts-goldyTALEN

Plasmid

#80330

Purpose

Updated TALEN vector; compatible with the Golden Gate TALEN assembly protocol. More stable backbone with reduced recombination compared to pT3Ts-goldyTALEN due to the removal of a lacZ sequence

Depositor

Jeffrey Essner

Article

Welker et al Hum Gene Ther. 2016 Jun;27(6):423-4. doi

Type

Empty backbone

Use

TALEN

Expression

Bacterial

Promoter

T3

Available Since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

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PKG I beta CAT

Plasmid

#16396

Depositor

Bernard Weinstein

Article

Deguchi et al Mol Cancer Ther. 2002 Aug . 1(10):803-9.

Insert

PKG1 beta (PRKG1 Human)

Tags

HA

Expression

Mammalian

Mutation

CAT: catalytic domain

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
pLBM0090

Plasmid

#172824

Purpose

Level 2 module containing mCHERRY-nVENUS HSP21-cVENUS

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

[35S-P19][35S-CTPSSU-mHSP21-nVENUS][35S-CTPSSU-mHSP21-X3FLAGcVENUS][35S-SP_SV40-CFP]

Use

Synthetic Biology

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3HA-AMOT p130∆PPxY1

Plasmid

#166711

Purpose

expresses AMOT with inactivating mutation in first PPxY motif in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

Angiomotin p130 L129A P130A

Tags

2xHA

Expression

Mammalian

Mutation

changed leucine 129 to alanine, proline 130 to al…

Promoter

CMV promoter

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_SPTAN1-1/1

Plasmid

#91292

Purpose

Protein expression and purification of human SH3 domain construct SPTAN1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SPTAN1-1/1 (SPTAN1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pBacPAK8-ElonginC

Plasmid

#29505

Depositor

Joan Conaway, Ronald Conaway

Article

Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

Insert

Elongin C (ELOC Human)

Expression

Insect

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pEBTetD-SNAP-FOP

Plasmid

#136797

Purpose

Mammalian expression of the centrosomal protein FGFR N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-FGFR

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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CP1041-ABE8e-dimer

Plasmid

#138499

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(WT)-ecTadA(8e)-nSpCas9 (CP1041)

Expression

Mammalian

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_SNX33-1/1

Plasmid

#91278

Purpose

Protein expression and purification of human SH3 domain construct SNX33-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SNX33-1/1 (SNX33 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-miRE_shBRD9_508-SV40-GFP

Plasmid

#75138

Purpose

Retroviral expression plasmid encoding shBRD9_508 (targeting human BRD)

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

shBRD9_508

Use

Retroviral

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only
minitalin (headR13DD)

Plasmid

#182994

Purpose

expression of minitalin wt in mammalian cells

Depositor

Vesa Hytönen

Article

Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

Insert

minitalin (Tln1 Mouse)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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A3H HapII-Cas9n-UGI

Plasmid

#119141

Purpose

Base editor made from APOBEC3H Haplotype II

Depositor

Reuben Harris

Article

Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

Insert

APOBEC3H Haplotype II-Cas9 nickase-UGI

Use

CRISPR

Mutation

None

Promoter

CMV

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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SaKKH-ABE8e-dimer

Plasmid

#138503

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(WT)-ecTadA(8e)-nSaCas9 (KKH)

Expression

Mammalian

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-24

Plasmid

#172750

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-24; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-24

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-9

Plasmid

#172742

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-9; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-9

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pIBA2-SpyTag-MBP

Plasmid

#107422

Purpose

Control construct for SpyCatcher experiments. Encodes MBP with N-terminal SpyTag, including OmpA signal peptide for periplasmic targeting.

Depositor

Jack Leo

Article

Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/

Insert

Maltose-binding protein (MalE)

Tags

OmpA signal peptide, SpyTag, and StrepII

Expression

Bacterial

Promoter

Tet

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-JNKsubDD-YPet-NES

Plasmid

#84633

Purpose

Encodes C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

JNKsubDD-YPet-NES

Tags

6xHis, Nuclear export signal (NES), T7 tag (gene …

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pBS U6 WIP RNAi

Plasmid

#11774

Depositor

Jack Strominger

Article

Krzewski et al J Cell Biol. 2006 Apr 10. 173(1):121-32.

Insert

WIP RNAi (WIPF1 Human)

Use

RNAi

Expression

Mammalian

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO GFP DNAJB7

Plasmid

#19503

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJB7 (DNAJB7 Human)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pC1300_pUB10_pco-nCASphi_E9t_V2_U6_AtPDS3_gRNA10

Plasmid

#197965

Purpose

T-DNA binary vector to express pco-nCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by U6 promoter.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Inserts

pco-nCasphi-2
AtPDS3 gRNA10

Use

CRISPR

Expression

Plant

Promoter

AtU6-26 and pUBQ10

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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COG4 shRNA

Plasmid

#163861

Purpose

shRNA targeting the coding sequences of COG4

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

component of oligomeric golgi complex 4 (COG4 Human)

Expression

Mammalian

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pLBT0160

Plasmid

#172812

Purpose

Level 1 module containing P19_P1 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-CDS1_P19-3U+TERNOS

Use

Synthetic Biology

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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LbABE8e-dimer

Plasmid

#138505

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(WT)-ecTadA(8e)-dLbCas12a

Expression

Mammalian

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pOpLexA::mini35s (GB0733)

Plasmid

#68180

Purpose

Provides the 4xLexA Operator fused to CaMV 35s minimal promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

4xOpLexA:mini35S

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-PPHLN1 ts2

Plasmid

#115878

Purpose

PPHLN1 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

PPHLN1 shRNA (PPHLN1 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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