We narrowed to 37,319 results for: kan
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Plasmid#172318PurposeCRISPR dCas9 SunTag system to target a variant of bacterial DNA methyltransferase MQ1 to install CG specific methylation to the FWA locus with three guide RNAsDepositorInsertg18_U6_g10_U6_g4_U6_NOS_MQ1(Q147L)_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyExpressionPlantAvailable sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET_His6-SUMO-TEV-TtCsm6
Plasmid#172486PurposeBacterial expression of codon-optimized His-SUMO-TtCsm6 with a TEV protease cleavage site.DepositorInsertT. thermophilus Csm6 (codon optimized) (csm6 Synthetic)
TagsHis-SUMO-TEVExpressionBacterialPromoterT7 promoter and lac operatorAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-sfGFP-DogTag Loop A
Plasmid#171779PurposeExpresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Val22 and Asn23DepositorInsertsfGFP-DogTag Loop A
TagsHis6ExpressionBacterialMutationDogTag and linkers inserted between residues Val2…PromoterT7Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pK27Sumo_His-SUMO-nsp10-nsp14 fusion (SARS-CoV-2)
Plasmid#169161PurposeTo express nsp10-nsp14 fusion protein in E. coliDepositorInsert14His-SUMO-nsp10-GGSGGS-nsp14 (ORF1ab SARS-CoV-2, Synthetic)
Tags14His-SUMO and Fusion protein of SARS-CoV-2 nsp10…ExpressionBacterialMutationCodon optimised for E. coliPromoterT5Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-26b-Nb127D01-HA-His
Plasmid#171566PurposeBacterial expression of HA-His-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-HA-His)DepositorPublicationInsertNb127D01-HA-His (CXCR2 Human)
TagsHA-tag/His-tag and PelB signal peptideExpressionBacterialAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
SQ171/pCSacB/ptRNA100
Bacterial strain#155206PurposeSQ171 strain supported by pCSacB and ptRNA100 plasmidsDepositorBacterial resistanceKanamycinAvailable sinceApril 27, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJYDNg
Plasmid#162452PurposeYeast display plasmid intended for expression of N-terminal fusions with Aga2p yeast agglutinin. The plasmid is carrying the eUnaG2 fluorescent protein and long 2G linker.DepositorPublicationTypeEmpty backboneUseShuttle vector bacteria/yeastTagsHa and myc and eUnaG2 fluorescent proteinAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mNeongreen-2xrGBD
Plasmid#129624PurposeVisualization of active RhoADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmNeongreen-2xrGBD
ExpressionMammalianPromoterCMVdelAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLX-B2 (GB3374)
Plasmid#160636PurposepLX series: pBBR1-based T-DNA binary vector for plant transformation (alpha1)DepositorTypeEmpty backboneUseSynthetic BiologyAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-A3A(N57Q)-BE3
Plasmid#158157PurposeT7 promoter bacterial expression plasmid for hAPOBEC3A(N57Q)-BE3 with N-terminal 6xHis-tag and C-terminal SV40 NLSDepositorInsert6xHis-hA3A(N57Q)-XTEN linker-hSpCas9n(D10A)-UGI-SV40NLS
Tags6xHis tag on N-terminusExpressionBacterialPromoterT7Available sinceNov. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET30a-His6-LOXCATmut
Plasmid#140085PurposeA control for His6-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)DepositorInsertA fusion of lactate oxidase from A. viridans and catalase from E. coli
TagsHis6ExpressionBacterialMutationMutation in H265A and R268A in LOX (used original…PromoterT7Available sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
mMaple-OMP25
Plasmid#141151PurposePhotoconvertible fluorescent protein targeted to the outer mitochondrial membrane (OMM) in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertOMP25 (Synj2bp Rat)
TagsmMapleExpressionMammalianMutationOMP25 c terminus from addgene plasmid #69598PromoterCMVAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pETM33_Nsp16
Plasmid#156482PurposeBacterial expression of Sars-CoV2 Nsp16 protein with His-tag and GST-tagDepositorInsertNsp16 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
TagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEE391
Plasmid#149281PurposeT-DNA encoding TRV2 with FT augmented gRNA targeting NbPDS3DepositorInsertp35S:TRV2_NbPDS3sgRNA_FT:tNos
ExpressionPlantPromoter35SAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
P305-FBP_4
Plasmid#139695PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, complete pathwayDepositorInsertpLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:tAtuOCS, pCmYLCV:NnHisps:tHsp, pAtuMas:NnLuz:trbcSE9
UseLuciferaseExpressionPlantPromotervariousAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV-mEGFP-CREB
Plasmid#137001PurposemEGFP-CREB donor for G-CREBDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCREB1 (Creb1 Rat)
TagsmEGFPExpressionMammalianPromoterCMVAvailable sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVAX1-BMP7
Plasmid#137910PurposeConstitutive mammalian overexpression vector for human bone morphogenetic protein 7 cDNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBMP7 (BMP7 Human)
ExpressionMammalianPromoterCMVAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLD1 translation factors
Plasmid#117760PurposeBioBrick encoding cistrons 1-13 in pET for preparation of PURE 3.0 translation system from E. coliDepositorInsertTranslation factor cistrons 1-13
UseEscherichia coliAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
[pSK131] pCOLADuet p14|MP1
Plasmid#136145Purposebacterial expression of p14 and MP1DepositorInsertp14 and MP1
ExpressionBacterialAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pME-NR2F2
Plasmid#138359PurposeGateway compatible middle entry clone contains human full length NFR2F2 gene with stop codonDepositorInsertHuman NR2F2
ExpressionMammalianAvailable sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by