We narrowed to 17,461 results for: She
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Plasmid#111858PurposeN-terminal SNAPf tag on BICDR1 for expression in Sf9 cells. 8xHis-ZZ-SNAPf N-terminal tag, TEV site to cleave 8xHis-ZZ.DepositorInsertBICDR1 (Bicdl1 Mouse)
UseTagsHis-tag, SNAPf-tag, TEV site, and ZZExpressionInsectMutationR250Q, Optimised for Sf9 expressionPromoterAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
Ratiometric FPX sensor for ERK kinase activity
Plasmid#60974PurposeFor imaging of ERK kinase activity using a single polypeptide RA-WW domain-ERK substrate-B protein and free GA-NES.DepositorInsertddRFP A-WW domain-ERK substrate-ddFP B
UseTagsERK substrate is before ddFP copy B, WW domain is…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pME-Sun1-2xsfGFP-6xMYC-pA (JDW 681)
Plasmid#224489PurposeGateway compatible middle entry clone containing Sun1 reporter fusion to 2 copies of sfGFP and 6 MYC tags (Nuclear envelope reporter and affinity tag)DepositorInsertSun1-2xsfGFP-6xMYC-pA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMT-Hrp48-ADARcd-V5
Plasmid#81172Purposeidentification of cell-specific targets of RNA binding proteinsDepositorInsertHrp48-ADARcd fusion (Hrb27C Fly)
UseTagsExpressionInsectMutationPromoterDrosophila metallothionein promoterAvailable sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8s-p10
Plasmid#162383PurposeLexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP8s
UseTags6xHisExpressionInsectMutationS26M F286Y Q315HPromoterhsp70Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
MBP-FUS_FL_6E
Plasmid#98655PurposeExpresses MBP-tagged full length phosphomimetic FUSDepositorInsertFUS (FUS Human)
UseTags6xHis-MBPExpressionBacterialMutation6 S/T to EPromoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
dSV40-NLS-dCas9-HA-NLS-NLS-10xGCN4
Plasmid#107310PurposeEncoding dCas9-10xGCN4 driven by dSV40 promoterDepositorInsertdSV40-dCas9-10xGCN4
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSW031
Plasmid#231144PurposeUsed to express cyclic sortase cW11 (recognizes APXTG motif) from E. coli.DepositorInsertS. aureus Sortase A mutant
UseTagsNostoc punctiform split intein C-terminal fragmen…ExpressionBacterialMutationP94S, E108Q, S116V, D160N, T164Q, V168M, L169H, D…PromoterT7Available sinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pD40-His/V5-c-MycT58A
Plasmid#45598DepositorInsertc-Myc T58A (Myc Mouse)
UseTags6xHis and V5ExpressionMammalianMutationT58APromoterCMVAvailable sinceJune 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 SARS-CoV-2 S D614
Plasmid#158074PurposeExpresses SARS-CoV-2 spike proteinDepositorInsertSARS-CoV-2 spike (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUNO mouse CD274 + 3'UTR WT full length
Plasmid#107012PurposeExpresses murine PD-L1 with the 3'UTRDepositorInsertCD274 (Cd274 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
FLAG-PRMT6(V86K/D88A)
Plasmid#164698PurposeExpression of PRMT6(V86K/D88A) with an N-terminal FLAG tagDepositorInsertPRMT6 (PRMT6 Human)
UseTagsFLAGExpressionMammalianMutationV86K, D88APromoterCMVAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO-puro-tetON-shOGDH 2497
Plasmid#110942PurposeTet-inducible shRNA targeting OGDHDepositorInsertshOGDH (OGDH Human)
UseLentiviralTagsExpressionMutationPromoterH1/TOAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSlick-Neo-RECK
Plasmid#121986PurposeTetracycline-inducible expression of untagged full-length RECKDepositorInsertRECK (RECK Human)
UseLentiviralTagsExpressionMutationPromoterTight TRE promoterAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
OA-1136J
Plasmid#153023PurposeExpress His-MBP-TEV-CasRx recombinant proteinDepositorInsertCasRx
UseTagsHis-MBP-TEVExpressionBacterialMutationPromoterT7Available sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO-puro-tetON-shOGDH 1714
Plasmid#110941PurposeTet-inducible shRNA targeting OGDHDepositorInsertshOGDH (OGDH Human)
UseRNAiTagsExpressionMutationPromoterH1/TOAvailable sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGP-20XUAS-IVS-Syn21-jGCaMP8m-p10
Plasmid#162387PurposeGal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP8m
UseTags6xHisExpressionInsectMutationA25G F286YPromoterhsp70Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO-puro-tetON-shOGDH 2956
Plasmid#110943PurposeTet-inducible shRNA targeting OGDHDepositorInsertshOGDH (OGDH Human)
UseLentiviral and RNAiTagsExpressionMutationPromoterH1/TOAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV_UdgX-APOBEC1-UdgX-NG-nCas9
Plasmid#163562PurposeMammalian CG-to-GC base editingDepositorInsertUdgX-APOBEC1-UdgX-NG-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…PromoterAvailable sinceDec. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-SomArchon-P2A-CoChR-Kv2.1
Plasmid#126945PurposeExpression of SomArchon-P2A-CoChR-Kv2.1 under Syn promoterDepositorInsertSomArchon-P2A-CoChR-Kv2.1
UseAAVTagsGFPExpressionMammalianMutationPromoterSynapsinAvailable sinceJune 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
FRT_htr2c(c)-GFP
Plasmid#79677Purposeserotonin receptor minigene with consensus splice sites that expresses the full length serotonin receptor with a C-terminal EGFPDepositorInsertserotonin receptor 2C (HTR2C Human)
UseTagsEGFP in FrameExpressionMammalianMutationsplice site of exon Vb in consensusPromoterCMVAvailable sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV_UdgX-Anc689-UdgX-NG-nCas9-RBMX
Plasmid#163554PurposeMammalian CG-to-GC base editingDepositorInsertUdgX-Anc689-UdgX-NG-nCas9-RBMX
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…PromoterAvailable sinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
GANES-DEVD-BNLS
Plasmid#50842PurposeExpresses a tandem green ddFP heterodimer in mammalian cells, construct 1 for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with a second plasmid RANLS.DepositorInsertddGFP A and ddGFP B
UseTagsNES sequence LALKLAGLDIGS placed after ddGFP A an…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pME Actin Vhh mNeonGreen-HA-pA (JDW 1248)
Plasmid#224500PurposeGateway compatible middle entry clone containing Actin nanobody fused to mNeonGreen with an HA tag (Visualizing actin, actin chromobody)DepositorInsertActin-Vhh-mNeonGreen-HA-pA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-CKAP4-WPRE-UbC-Emerald
Plasmid#225943PurposeLentiviral vector plasmid expressing human cytoskeleton associated protein 4 (CKAP4) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorInsertsUseLentiviralTagsExpressionMammalianMutationPromoterSFFV and UbCAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TRE-FH-TET3 short
Plasmid#166916PurposeLentivirus for inducible overexpression of human TET3 short isoform lacking CXXC domain (NM_001366022.1)DepositorInsertTET3 short isoform
UseLentiviralTagsHA TagExpressionMammalianMutationChanged nucleotide 18 (G) to (A) to delete FseI r…PromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
HiBiT-FZD6
Plasmid#195844PurposeExpresses human FZD6 with an N-terminal HiBiT; contains M345LDepositorInsertFZD6 (FZD6 Human)
UseTagsHiBiTExpressionMammalianMutationM345LPromoterCMVAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNK5712
Plasmid#219752PurposepGAP-like vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 under control of GAP promoter, for yeast expressionDepositorInsertpGAP - nnH3H_v2 - tAOX
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK5785
Plasmid#219753PurposepGAP-like vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 under control of GAP promoter, for yeast expressionDepositorInsertpGAP - nnLuz_v4 - tAOX
UseLuciferaseTagsExpressionYeastMutationPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV_UdgX-APOBEC1-UdgX-HF-NG-nCas9
Plasmid#163561PurposeMammalian CG-to-GC base editingDepositorInsertUdgX-APOBEC1-UdgX-HF-NG-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)N497A, R661A, Q695A, Q926A//L111R, D1…PromoterAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-jYCaMP1
Plasmid#135423PurposeYellow protein calcium sensor expressed under human Synapsin1 promoter, for AAV production or direct transfectionDepositorInsertjYCaMP1
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Puro-IKBalpha-wt
Plasmid#15290DepositorInsertinhibitor of Kappa B alpha (NFKBIA Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8f-p10
Plasmid#162385PurposeLexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP8f
UseTags6xHisExpressionInsectMutationQ315LPromoterhsp70Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB1H2 UV5 Zif268-cons (2-hybrid bait plasmid)
Plasmid#128164PurposeUV5 promoter drives expression of Zif268-consensus peptide fusion. Pair with pGHUC w-ERBIN as a positive control (turns on HIS3/GFP).DepositorInsert10 amino acid glycine/serine linker + ERBIN PDZ consensus ligand (WETWV)
UseSynthetic BiologyTagsFLAG and zif268ExpressionBacterialMutationPromoterUV5Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8m-p10
Plasmid#162384PurposeLexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP8m
UseTags6xHisExpressionInsectMutationA25G F286YPromoterhsp70Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNK5867
Plasmid#219751PurposepGAP-like vector encoding hispidin-synthase from Mycena citricolor under control of GAP promoter, for yeast expressionDepositorInsertpGAP - mcitHispS - tAOX
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK1508
Plasmid#219750PurposepGAP-like vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA under control of GAP promoter, for yeast expressionDepositorInsertpGAP - npgA - tAOX
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
NESRA-DEVD-BNLS
Plasmid#50849PurposeExpresses a tandem red ddFP heterodimer in mammalian cells, construct 1 for a red to green colour switch-based fluorescent caspase-3 biosensor, used together with a second plasmid GANLS.DepositorInsertddRFP A and ddRFP B
UseTagsNES sequence LQKKLEELELDE placed after ddGFP A an…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
Human FLAG-UAF1 S170Y
Plasmid#217619PurposeMammalian expression of FLAG tagged mutant (UAF1 S170Y) UAF1DepositorInsertUAF1 (WDR48 Human)
UseTagsExpressionMammalianMutationS170YPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only