We narrowed to 66,557 results for: des.1
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Plasmid#118718PurposeThe donor (YPet(short)) only control for the no force control of the F40-based human desmoplakin II tension sensor provides the donor only lifetime used in lifetime measurements to determine FRET.DepositorInserthuman Desmoplakin II (1-1353)-[YPet(short)-F40-mCherry(Y72L)] (DSP Human)
UseRetroviralTagsYPet(short)-F40-mCherry(Y72L)ExpressionMammalianMutationtruncation after aa1353; Y72L mutation in mCherry…PromoterCMVAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
CaMKII-somBiPOLES-mCerulean (AAV Retrograde)
Viral Prep#154948-AAVrgPurposeReady-to-use AAV Retrograde particles produced from CaMKII-somBiPOLES-mCerulean (#154948). In addition to the viral particles, you will also receive purified CaMKII-somBiPOLES-mCerulean plasmid DNA. CamKII-driven expression of soma-targeted BiPOLES for optogenetic inhibition (blue light) and activation (red light). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIIa(0.4)TagsmCeruleanAvailable SinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB09-TRE-beta-globin-miR-124-EF1a-GFP
Plasmid#117318PurposeOverexpression of miR-124 in eurkaryotic cells, encoded in a human beta-globin intronDepositorInserthsa-miR-124-1 (MIR124-1 Human)
UseTransposon-mediated integrationTagsGFPExpressionMammalianPromoterTet-inducible promoterAvailable SinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+-Tornado-split-nLuc
Plasmid#212612PurposeFor the expression of a split nLuc that produces luminescence only when circularized. To test your IRES of interest, clone into the EcoRI, BsiWI sites.DepositorInsertTornado-CVB3-split-nLuc
UseLuciferaseExpressionMammalianMutationC373T mutation in CVB3PromoterCMVAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-mNeongreen
Plasmid#182387PurposeBacterial expression of Vesicle Nucleating peptide-mNeongreen fusionDepositorInsertVNp-mNeongreen-His
TagsVesicle Nucleating peptide (VNp)ExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gαq-LgB97
Plasmid#134360PurposeNanoluc complementation assay. Expression of Gαq protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gαq. Addition of the HA epitope at N terminus of Gαq.DepositorAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE
Plasmid#131003Purposebicistronic AAV vector to express GCaMP6m and soma-targeted ChRmine under the control of CamKIIa promoterDepositorInsertChRmine
UseAAVTagsKv2.1-HAPromoterCaMKIIaAvailable SinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Hygro(+)-mscGAS
Plasmid#102607PurposeMammalian expression of mouse cGASDepositorAvailable SinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 - PP1g - Flag - BirA* (AA2-140)
Plasmid#86886PurposeN-terminal part of the split-BioIDDepositorInsertPP1g - FLAG - BirA (AA2-140)
TagsFLAG tagExpressionMammalianMutationBirA Amino Acids 2-140Available SinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-KozATG-dL5-2XG4S-mCer3
Plasmid#73207PurposeExpresses dL5(E52D)-mCer3 fusion protein in cytosol of mammalian cells, penetrates nucleus. (MBIC5, dL5**, FAP)DepositorInsertKozATG-dL5-2XG4S-mCer3
TagsThe FAP and mCerulean3 are fused with 2 copies of…ExpressionMammalianMutationThe dL5** FAP is E50D, L89S, also known as E52D/L…PromoterCMVAvailable SinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL4.10-Claudin-1
Plasmid#46387PurposeClaudin-1 promoter reporter assayDepositorAvailable SinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TAT-1-101-FLAG
Plasmid#35981DepositorInserttransactivating protein (tat )
TagsFlagExpressionMammalianMutationtat from HIV1 101 amino acidsAvailable SinceSept. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mBATF
Plasmid#34575DepositorAvailable SinceDec. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1 GFP B56 gamma3 shRNA 14-1
Plasmid#10681DepositorAvailable SinceApril 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
MSCVpic2neo-hTERT-p16 shRNA
Plasmid#164920PurposeExpresses hTERT cDNA and p16 shRNA in mammalian cellsDepositorUseRetroviralTagsHA tagAvailable SinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PGC1(1-400)
Plasmid#1029DepositorAvailable SinceSept. 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
PU.1-S148A LZRS
Plasmid#44633DepositorInsertPU.1 S148A (Spi1 Mouse)
UseRetroviralTagsEGFP and IRESExpressionMammalianMutation148 mutation (S to A)Available SinceApril 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro-cGASsh4
Plasmid#127646PurposeKnock-down of human cGASDepositorInsertcGAS shRNA (CGAS Human)
UseLentiviralAvailable SinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-TetON-Puro-HIF1a 0819
Plasmid#118704PurposeLentivirus for expression of shRNA1 against human HIF1a (Dox-inducible)DepositorInsertshRNA against human HIF1a
UseLentiviralAvailable SinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 GfaABC1D NAPA-A SV40
Plasmid#92281PurposeFRET probe; extracellular membrane tethered eGFPDepositorInsertNeuron-astrocyte proximity assay - Astrocyte
UseAAVPromoterGfaABC1DAvailable SinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only