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pMTG06-Amp-FRT

Plasmid

#128275

Purpose

Deg-LITer2.0 gene circuit with the following architecture: CMV-TetOx2-TetR-LOV-RRRG-P2A-GFP

Depositor

Gabor Balazsi

Article

Guinn et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

Inserts

TetR-LOV-RRRG
EGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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BS-TRP1-TetOff(7 repeats)-mRuby3-Pho8

Plasmid

#207031

Purpose

Tet-Off controlled mRuby3-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Pho8

Tags

mRuby3

Expression

Yeast

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/TO-myc-BirA*-NES

Plasmid

#112714

Purpose

For tetracycline-inducible expression of myc-BirA*-NES in mammalian cells. For BioID studies.

Depositor

Daniel Schoenberg

Article

Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

Insert

HIV Rev nuclear export signal (NES)

Expression

Mammalian

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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SnFR-gamma8

Plasmid

#165496

Purpose

Green fluorescent glutamate reporter fused to TARP gamma-8 for mammalian expression.

Depositor

Andrew Plested

Article

Hao et al Elife. 2023 Jan 9;12:e84029. doi: 10.755

Insert

iGluSnFR extracellular domain fused to gamma-8 via NETO2 TM domain (Cacng8 Synthetic, Rat)

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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C1-FT-mCitrine-EAAARx8-CaaX

Plasmid

#155224

Purpose

MPAct with increased search radius

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

F-tractin (aa9-52 of rat ITPKA) fused to mCitrine C-term tagged with codon optimized (EAAAR)x8 and CaaX seq derived from Kras4b

Tags

mCitrine

Expression

Mammalian

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pDendra2-H2a

Plasmid

#157816

Purpose

Fluorescent reporter for histone H2A tracking

Depositor

Xin Chen

Article

Ma et al Cell Rep. 2020 Aug 11;32(6):108003. doi:

Insert

H2A (H2ac13 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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mCHOP10.pCDNA1

Plasmid

#21899

Depositor

David Ron

Article

Ron et al Genes Dev. 1992 Mar;6(3):439-53. doi: 10

Insert

CHOP10 (Ddit3 Mouse)

Expression

Mammalian

Available Since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only
pMP71-tCD19-LLO-SIINFEKL

Plasmid

#174598

Purpose

expresses the extracellular and transmembrane domain of murine CD19 with a C terminal fusion of the LLO190 and SIINFEKL epitopes

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

membrane bound CD19, LLO antigen, SIINFEKL (ovalbumin) antigen

Expression

Mammalian

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pSW002-PpsbA-E2-Crimson

Plasmid

#111258

Purpose

Broad host-range bacterial expression vector with constitutive PsbA promoter; for tagging bacteria with E2-Crimson

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

E2-Crimson

Expression

Bacterial

Promoter

PpsbA

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-SnFR-gamma8-minWPRE

Plasmid

#165498

Purpose

AAV for glutamate reporter fused to TARP gamma-8

Depositor

Andrew Plested

Article

Hao et al Elife. 2023 Jan 9;12:e84029. doi: 10.755

Insert

iGluSnFR extracellular domain fused to TARP gamma-8 via NETO2 TM domain (Cacng8 Synthetic, Rat)

Use

AAV

Promoter

hSynapsin

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a(TIGIT)

Plasmid

#177902

Purpose

Bacterial expression of his10-tagged human TIGIT Intracellular Domain

Depositor

Ron Vale

Article

Hui et al Science. 2017 Mar 31;355(6332):1428-1433

Insert

TIGIT (aa 163-244 only) (TIGIT Human)

Tags

10xHis-Thrombin cut site-T7 tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pRJPaph-lacZYA

Plasmid

#67037

Purpose

Plasmid for Paph-lacZYA integration downstream of B. diazoefficiens (B. japonicum) scoI

Depositor

Julia Vorholt

Article

Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

Inserts

B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
lacZYA

Use

Conjugative bacterial vector

Available Since

Oct. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pACYC-Cas12g1-noncoding

Plasmid

#120880

Purpose

Expresses concatenated noncoding sequences surrounding the CRISPR-Cas12g1 system in the native genomic loci; expresses the tracrRNA required for activity.

Depositor

Arbor Biotechnologies

Article

Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

Insert

Noncoding sequences for CRISPR-Cas12g1

Use

CRISPR

Expression

Bacterial

Promoter

lac

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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Ad-CBR (GFP/cAPC)

Plasmid

#16576

Depositor

Bert Vogelstein

Article

Shih et al Cancer Res. 2000 Mar 15. 60(6):1671-6.

Insert

cAPC (APC Human)

Use

Adenoviral

Tags

EGFP

Expression

Mammalian

Mutation

Residues 958-2076

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
CrBlue ---- pcDNA3.1

Plasmid

#62917

Purpose

CrBlue is a synthetic chimera comprising the blue opsin backbone with the intracellular loops and the C terminus of the jellyfish opsin, created to introduce spectral selectivity.

Depositor

Narasimhan Gautam

Article

Karunarathne et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

Insert

CrBlue

Expression

Mammalian

Promoter

CMV

Available Since

April 13, 2015

Availability

Academic Institutions and Nonprofits only
pSW002-Pc-TorA(sp)

Plasmid

#111253

Purpose

Broad host-range bacterial expression vector with constitutive Pc promoter followed by the E. coli TorA signal peptide

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

TorA signal peptide

Expression

Bacterial

Promoter

Pc

Available Since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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T7 promoter + rpsL reporter plasmid

Plasmid

#156455

Purpose

A mutational reporter plasmid that can gain Streptomycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.

Depositor

Matthew D. Shoulders

Article

Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

Inserts

rpsL
TcR

Expression

Bacterial

Mutation

ATG start codon mutated to ACG

Promoter

tac and tet

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pRJPaph-gusA

Plasmid

#67036

Purpose

Plasmid for Paph-gusA integration downstream of B. diazoefficiens (B. japonicum) scoI

Depositor

Julia Vorholt

Article

Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

Inserts

B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
gusA

Use

Conjugative bacterial vector

Available Since

Oct. 30, 2015

Availability

Academic Institutions and Nonprofits only

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PliaI

Plasmid

#55177

Purpose

Bacitracin-inducible promoter

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Insert

liaI promoter

Use

Synthetic Biology; Bacillus biobrick box

Promoter

liaI

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-DrU6a:sgRNA-com-Ds

Plasmid

#119077

Purpose

To clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x com stem loops

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:2xCom-sgRNA

Expression

Bacterial

Promoter

Zebrafish U6a promoter

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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