We narrowed to 35,527 results for: Mut

1. pTH363-SUP45-F401Y

   Plasmid

   #29394

   Depositor

   Tobias von der Haar

   Article

   Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

   Insert

   SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   F401Y mutant gene including genomic sequence 1432…

   Promoter

   Available Since

   May 5, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pTH364-SUP45-Y410F

   Plasmid

   #29395

   Depositor

   Tobias von der Haar

   Article

   Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

   Insert

   SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Y410F mutant gene including genomic sequence 1432…

   Promoter

   Available Since

   May 5, 2011

   Availability

   Academic Institutions and Nonprofits only

3. 23SrRNA.RAV12(wt).2508-2580.GAAAC.G2553U

   Plasmid

   #26191

   Depositor

   David McKay

   Article

   Hardin et al J Mol Biol. 2010 Sep 17;402(2):412-27. d

   Insert

   23S ribosomal RNA fragment (modified) with G2553U mutation
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   fragment 2508-2580 of E. coli rRNA, with nucleoti…

   Promoter

   Available Since

   Oct. 28, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR221-hSOX10-G1238A-C1240T

   Plasmid

   #24739

   Depositor

   William Pavan

   Article

   Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

   Insert

   (sex determining region Y)-box 10 (SOX10 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   G replaced with A at position 1238 and C replaced…

   Promoter

   Available Since

   May 18, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3.1(+)_4x(U6 tRNA M15)_CMV NESPylRS(AF)

   Plasmid

   #182652

   Purpose

   Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & 4x M15 tRNACUA used for amber codon suppression in mammalian cells

   Depositor

   Ivana Nikić-Spiegel

   Article

   Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

   Inserts

   codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
   4xU6-tRNAM15
   

   Use

   Tags

   nuclear export signal (NES)

   Expression

   Mammalian

   Mutation

   Y306A/Y384F (AF) double mutant of Methanosarcina …

   Promoter

   CMV and U6

   Available Since

   May 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA 3.1 KBTBD4 WT

   Plasmid

   #184627

   Purpose

   Mammalian expression vector with N-terminal 3xFlag for KBTBD4 WT expression in mammalian cells

   Depositor

   Vincenzo D'Angiolella

   Article

   Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

   Insert

   N-terminal 3xFlag tagged KBTBD4 WT (KBTBD4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   no mutation

   Promoter

   CMV

   Available Since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti_EF1a_Flag_TP53_R248W

   Plasmid

   #183114

   Purpose

   Expresses flag-tagged p53 with R248W mutation in mammalian cells

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   TP53 (TP53 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed arginine 248 to tryptophan

   Promoter

   EF1a

   Available Since

   April 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBOB-CAG-SARS-CoV2-Spike D614G-HA

   Plasmid

   #158761

   Purpose

   3rd Generation lentiviral vector expressing the codon optimized SARS-CoV2 Spike Glycoprotein D614G high infectivity mutant with a C-terminal HA tag generated by Junko Ogawa & Gerald M Pao

   Depositor

   Gerald Pao

   Article

   Ogawa et al bioRxiv. 2020 Jul 22. doi: 10.1101/2020.

   Insert

   SARS-CoV2 Spike Glycoprotein (S SARS-CoV2 hCoV19_USA EPI_ISL_414366 (GISAID))
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Spike D614G

   Promoter

   CAG

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-IDH2-R172K

   Plasmid

   #87927

   Purpose

   Expresses IDH2 (R172K mutant) in mammalian cells

   Depositor

   Reproducibility Project Cancer Biology

   Article

   Showalter et al Elife. 2017 Jun 27;6. pii: e26030. doi:

   Insert

   isocitrate dehydrogenase (NADP(+)) 2, mitochondrial (IDH2 Human)
   

   Use

   Tags

   none

   Expression

   Mammalian

   Mutation

   R172K

   Promoter

   CMV

   Available Since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIS1 mLPIN1

    Plasmid

    #60803

    Purpose

    Luciferase reporter to measure miR-155 mediated differential repression. Contains LPIN1 3' UTR and mutated miR-155 sites

    Depositor

    David Bartel

    Article

    Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi

    Insert

    LPIN1 3'UTR and mutated miR-155 binding site (LPIN1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    mutated miR-155 binding site

    Promoter

    thymidine kinase (HSV-TK promoter)

    Available Since

    Oct. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-N-HA-NEK7 K64M

    Plasmid

    #75143

    Purpose

    Expresses N-terminal HA-tagged NEK7 (with a K64M substitution; kinase dead) in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Lys 64 mutated to Met; kinase inactive and F236L …

    Promoter

    CMV

    Available Since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. AID-C12*-BE4max

    Plasmid

    #216729

    Purpose

    Expresses a BE4max base editing construct comprised of the hyperactive AID-C12* and nCas9 (Cas9 with D10A mutation) in mammalian cells.

    Depositor

    Rahul Kohli

    Article

    Berrios et al Nucleic Acids Res. 2024 Jan 23:gkae024.

    Insert

    AID-C12*-BE4max (AICDA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCW57.1 KBTBD4 WT

    Plasmid

    #184624

    Purpose

    Doxycycline inducible lentiviral vector for N-terminal Flag tagged KBTBD4 WT expression in mammalian cells

    Depositor

    Vincenzo D'Angiolella

    Article

    Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

    Insert

    N-terminal Flag tagged KBTBD4 WT (KBTBD4 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    no mutation

    Promoter

    TRE promoter, Tet ON

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT7-7 asyn G51D

    Plasmid

    #105747

    Purpose

    Bacterial expression of mutant human alpha synuclein

    Depositor

    Hilal Lashuel

    Article

    Fares et al Hum Mol Genet. 2014 Sep 1;23(17):4491-50

    Insert

    alpha-synuclein G51D (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G51D

    Promoter

    T7

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-N-Myc_DED/AAA USP7

    Plasmid

    #131253

    Purpose

    Mammalian expression of an N-terminally Myc-tagged USP7 mutant, with mutations in UBL2 (DE758AA_D764A)

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Contains point mutations that convert USP7 aspart…

    Promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. G protein alpha-s(alpha3beta5)

    Plasmid

    #55799

    Purpose

    This G protein alpha-s mutant exhibits increased affinity for and decreased ability to be activated by Gs-protein-coupled receptors as well as decreased ability to activate adenylyl cyclase.

    Depositor

    Catherine Berlot

    Article

    Berlot et al J Biol Chem. 2002 Jun 7;277(23):21080-5.

    Insert

    G protein alpha-s with alpha-i2 substitutions in alpha3/beta5 loop (Gnas Rat)
    

    Use

    Tags

    internal EE epitope (residues 189-194 in alpha-s …

    Expression

    Mammalian

    Mutation

    N271K, K274D, R280K, T284D, I285T in alpha-s sequ…

    Promoter

    CMV

    Available Since

    July 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1/FGFR2-S252W(Apert) myc

    Plasmid

    #33249

    Depositor

    Alka Mansukhani, Claudio Basilico

    Article

    Mansukhani et al J Cell Biol. 2000 Jun 12. 149(6):1297-30

    Insert

    FGFR2 (Fgfr2 Mouse)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Mutation S252W in FGFR2 found in Apert syndrome. …

    Promoter

    CMV

    Available Since

    Dec. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pTriEx-PA-Rac1-C450A

    Plasmid

    #22025

    Purpose

    Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1-C450A (RAC1 Human, Avena sativa (oat))
    

    Use

    Tags

    6X His

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    LOV domain contains light-insensitive mutation C4…

    Promoter

    CMV, p10

    Available Since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

19. pTriEx-PA-Rac1-I539E

    Plasmid

    #22026

    Purpose

    Expression of photoactivatable Rac1 ('lit' state mutant)

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1-I539E (RAC1 Human, Avena sativa (oat))
    

    Use

    Tags

    6X His

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    LOV domain contains 'lit state' mutatio…

    Promoter

    CMV, p10

    Available Since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

20. LV-AR-AAFF

    Plasmid

    #171218

    Purpose

    Lenti-viral expression of human androgen receptor DNA-binding mutant: K580A-V581A, mutations in the P-box of the zinc finger 1 at the KVFF motif.

    Depositor

    Steven Balk

    Article

    Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:

    Insert

    AR-AAFF (AR Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    AR-K580A-V581A

    Promoter

    EF-1a

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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