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pAAV-CAG-RiboL1-jGCaMP8m

Plasmid

#192613

Purpose

AAV transfer plasmid for CAG mediated expression of soma-targeted (RiboL1) jGCaMP8m.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

RiboL1-jGCaMP8m

Use

AAV

Tags

6xHis

Expression

Mammalian

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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peCALWY-4

Plasmid

#22236

Depositor

Maarten Merkx

Article

Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

Insert

eCALWY-4 Zn(II) sensor (ATOX1 Human, GFP's)

Expression

Mammalian

Mutation

C418S mutation linker consists of 9 GGSGGS repea…

Available Since

Nov. 25, 2009

Availability

Academic Institutions and Nonprofits only

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mCherry-Lrrc59

Plasmid

#226565

Purpose

Encodes Lrrc59 protein laebeled with mCherry

Depositor

Gia Voeltz

Article

Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

Insert

Lrrc59 (Leucine-rich repeat containing protein 59) (LRRC59 Human)

Tags

mCherry

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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3XFlagNICD4

Plasmid

#20186

Depositor

Raphael Kopan

Article

Ong et al J Biol Chem. 2006 Feb 24. 281(8):5106-19

Insert

Notch 4 Intracellular Domain (Notch4 Mouse)

Tags

3X Flag

Expression

Mammalian

Mutation

Contains murine Notch 4 Intracellular Domain (Val…

Promoter

CMV

Available Since

Dec. 13, 2012

Availability

Academic Institutions and Nonprofits only
pEasyCas9

Plasmid

#184909

Purpose

Expresses the Streptococcus pyogenes Cas9 protein

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

Cas9

Use

CRISPR

Expression

Bacterial and Yeast

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pFSW GAP43-jRGECO1a P2A Syp-Halo

Plasmid

#185565

Purpose

Expression of axonally-targeted jRGECO1a, a red Ca2+ sensor, along with synaptophysin-HaloTag in neuronal cells, separated by a P2A sequence

Depositor

Edwin Chapman

Article

Bradberry et al J Physiol. 2022 Feb;600(3):645-654. doi:

Insert

GAP43-jRGECO1a P2A Syp-Halo

Use

Lentiviral

Tags

HaloTag

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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siRNA-resistant SigmaR1-mCherry

Plasmid

#226567

Purpose

Encodes siRNA resistant SigmaR1 protein labeled with mCherry

Depositor

Gia Voeltz

Article

Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

Insert

SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)

Tags

mCherry

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag-gp130dimer

Plasmid

#174394

Purpose

Cytosolic domain of gp130 with C-C linker and Leu zipper for constitutive dimerization and activation (from Stuhlmann-Laeisz et al. 2006).

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

IL6ST cytosolic domain with leucine zipper & C-C linker

Tags

Flag

Expression

Mammalian

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ166-OsPE2

Plasmid

#141080

Purpose

Gateway entry clone for CRISPR-Cas9(H840A) prime editing

Depositor

Yiping Qi

Article

Tang et al Mol Plant. 2020 Mar 25. pii: S1674-2052(

Insert

zCas9(H840A)-OsM-MLV-RT

Use

CRISPR; Gateway compatible zcas9(h840a)-osm-mlv-rt

Tags

3x FLAG, NLS and NLS

Expression

Plant

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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STOP-eGFP-ROSA26TV

Plasmid

#11739

Depositor

Klaus Rajewsky

Article

Sasaki et al Immunity. 2006 Jun . 24(6):729-39.

Insert

STOP-eGFP-ROSA26TV

Use

Cre/Lox; /stop

Available Since

May 17, 2006

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP104-melanopsin-mCherry

Plasmid

#122630

Purpose

AAV-mediated expression of melanopsin-mCherry under the GFAP promoter

Depositor

Edward Boyden

Article

Mederos et al Glia. 2019 May;67(5):915-934. doi: 10.10

Insert

melanopsin-mCherry

Use

AAV

Tags

mCherry

Expression

Mammalian

Promoter

GFAP104

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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408 pSG5L HA E2F1

Plasmid

#10736

Depositor

William Sellers

Article

Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

Insert

E2F1 (E2F1 Human)

Tags

HA

Expression

Mammalian

Available Since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

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AAV-hRedOpsin-TGFB1

Plasmid

#164427

Purpose

AAV plasmid expressing TGFB1 in photoreceptors

Depositor

Connie Cepko

Article

Wang et al J Clin Invest. 2020 Aug 3;130(8):4360-43

Insert

Tgfb1 (Tgfb1 Mouse)

Use

AAV

Expression

Mammalian

Promoter

human red opsin

Available Since

Nov. 20, 2021

Availability

Academic Institutions and Nonprofits only

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hGip-R

Plasmid

#14942

Depositor

Bernard Thorens

Article

Gremlich et al Diabetes. 1995 Oct . 44(10):1202-8.

Insert

Gip-R (GIPR Human)

Expression

Mammalian

Mutation

Short form of the receptor

Available Since

June 7, 2007

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-FLEX-RiboL1-jGCaMP8f

Plasmid

#192618

Purpose

AAV transfer plasmid for neuronal expression of Cre-dependent (FLEX), soma-targeted (RiboL1) jGCaMP8f.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

FLEX-RiboL1-jGCaMP8f

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-human-Caspase-1

Plasmid

#183359

Purpose

Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-1 (CASP1 Human)

Use

Retroviral

Tags

Myc

Promoter

MESV

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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PERK 2: PERK.K618A.9E10.pCDNA

Plasmid

#21815

Depositor

David Ron

Article

Harding et al Nature. 1999 Jan 21. 397(6716):271-4.

Insert

PERK (K618A) (Eif2ak3 Mouse)

Tags

Myc (9E10)

Expression

Mammalian

Mutation

kinase dead K618A mutant

Available Since

Oct. 14, 2009

Availability

Academic Institutions and Nonprofits only

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GOLPH3 shRNA

Plasmid

#163860

Purpose

shRNA targeting the coding sequences of GOLPH3

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

golgi phosphoprotein 3 (GOLPH3 Human)

Expression

Mammalian

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-N2c_envA-IRES-Neo

Plasmid

#172368

Purpose

Generation of envA-expressing stable cell lines, resistant to the antibiotic Neomycin (Gentamicin/G418)

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

envA + Neo

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pDA025

Plasmid

#131384

Purpose

ORFeus expression, mutant

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

ORFeus

Tags

ORF2-3xFlag

Expression

Mammalian

Mutation

H230A En

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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