We narrowed to 4,734 results for: MLS

1. pL-rpb1-R320C

   Plasmid

   #10987

   Depositor

   Jeff Strathern

   Article

   Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

   Insert

   rpb1-R320C (RPO21 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Mutation 958 C to T in the RPB1 ORF resulting i…

   Promoter

   Available since

   Dec. 16, 2005

   Availability

   Academic Institutions and Nonprofits only

2. pL-rpb1-E1230K

   Plasmid

   #10990

   Depositor

   Jeff Strathern

   Article

   Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

   Insert

   rpb1-E1230K (RPO21 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Mutation 3688 G to A in the RPB1 ORF resulting in…

   Promoter

   Available since

   Dec. 16, 2005

   Availability

   Academic Institutions and Nonprofits only

3. cASIC1 concatemer - Subunit A

   Plasmid

   #233129

   Purpose

   To create cASIC1 channels of defined stoichiometry in mammalian cells

   Depositor

   David MacLean

   Article

   Purohit et al Biophys J. 2024 Oct 15;123(20):3507-3518

   Insert

   ASIC1 (ASIC1 Chicken)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJJB170

   Plasmid

   #218591

   Purpose

   Express engineered TFs Adr1c(S230A), Pip2c, and Oaf1c

   Depositor

   John Dueber

   Article

   Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

   Insert

   Adr1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Adr1(S230A), Pip2(1-168 + 2,497-2,988), Oaf1(1-30…

   Promoter

   Available since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJJB1340

   Plasmid

   #218593

   Purpose

   Express a red fluorescent protein to the peroxisome membrane via tethering to a truncated Pex22. Also overexpresses Pex11

   Depositor

   John Dueber

   Article

   Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

   Insert

   Pex22
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Pex22(1-36)

   Promoter

   Available since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSKT001

   Plasmid

   #218594

   Purpose

   Express degron assay: Degradation tag-YFP-ePTS1

   Depositor

   John Dueber

   Article

   Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

   Insert

   YFP
   

   Use

   Tags

   UbiY degradation tag and ePTS1

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJJB1357

   Plasmid

   #218595

   Purpose

   Express Pex11 and the degron assay: Degradation tag-YFP-ePTS1

   Depositor

   John Dueber

   Article

   Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

   Insert

   Pex11
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKS1461

   Plasmid

   #218587

   Purpose

   Express peroxisomal Erg12p, Erg8p, and Mvd1p to convert mevalonate to IPP

   Depositor

   John Dueber

   Article

   Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

   Insert

   Erg12
   

   Use

   Tags

   ePTS1

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-H2B-MCS

   Plasmid

   #184041

   Purpose

   Cloning of GFP-H2B fusion proteins

   Depositor

   Lienhard Schmitz

   Article

   Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

   Type

   Empty backbone

   Use

   Tags

   GFP and H2B

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJR152

    Plasmid

    #196280

    Purpose

    Cassette used in dual sgRNA library generation

    Depositor

    Rebecca Voorhees

    Article

    Guna et al BMC Genomics. 2023 Oct 30;24(1):651. doi

    Insert

    hU6-sgRNA-CR3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. aCE89

    Plasmid

    #204494

    Purpose

    TREtight promoted WBeta and PhlF promoted PhiC31 without PEST tags

    Depositor

    Ron Weiss

    Article

    Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

    Inserts

    φC31 integrase
    WBeta recombinase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    PhIF and TREtight

    Available since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. aCE90

    Plasmid

    #204495

    Purpose

    TREtight promoted WBeta and PhlF promoted PhiC31 with PEST tags

    Depositor

    Ron Weiss

    Article

    Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

    Inserts

    phiC31 recombinase
    WBeta recombinase
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    PhIF and TREtight

    Available since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-REPORT(EF1a no GFP)

    Plasmid

    #172339

    Purpose

    mnucTomato

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    monomeric nuclear Tomato
    HygR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3x SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-REPORT(EF1a)TetR

    Plasmid

    #172352

    Purpose

    Tet Repressor reporter

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    TetR
    d2eGFP
    HygR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3x SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSCV-mouse Mdm2 mutant M3 (399-489)-IRES-GFP

    Plasmid

    #180490

    Purpose

    Role of Mdm2 variants in tumorigenesis

    Depositor

    Martine Roussel

    Article

    Dang et al Cancer Res. 2002 Feb 15;62(4):1222-30.

    Inserts

    Mouse Mdm2 mutant
    Mouse Mdm2 mutant
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    399-489

    Promoter

    MSCV

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. MSCV-mouse Mdm2 mutant M2 (299-489)-IRES-GFP

    Plasmid

    #180489

    Purpose

    Role of Mdm2 variants in tumorigenesis

    Depositor

    Martine Roussel

    Article

    Dang et al Cancer Res. 2002 Feb 15;62(4):1222-30.

    Insert

    Mouse Mdm2 mutant
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    299-489

    Promoter

    MSCV

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. DT039A

    Plasmid

    #159400

    Purpose

    Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli J23117 (weak) promoter

    Depositor

    Sheref Mansy

    Article

    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

    Insert

    pr.J23117-sfGFP
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    J23117 (weak) promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. DT038A

    Plasmid

    #159399

    Purpose

    Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli J23106 (medium) promoter

    Depositor

    Sheref Mansy

    Article

    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

    Insert

    pr.J23106-sfGFP
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    J23106 (medium) promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCW-SPIC

    Plasmid

    #162825

    Purpose

    Doxycycline-inducible overexpression of human SPIC

    Depositor

    Matthew Steinhauser

    Article

    Fazeli et al Mol Metab. 2020 Sep 28;42:101082. doi: 1

    Insert

    SPIC (SPIC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet ON

    Available since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRS314_U6_A34G_A35G

    Plasmid

    #81232

    Purpose

    U6 telestem mutant (A34G/A35G) for in vivo studies

    Depositor

    Aaron Hoskins

    Article

    Rodgers et al Nucleic Acids Res. 2016 Dec 15;44(22):10

    Insert

    U6 RNA with A34G/A35G mutation (snR6 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    U6 telestem mutant (A34G/A35G)

    Promoter

    Available since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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