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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,742 results for: grna

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Showing: 561 - 580 of 16742 results

  5. Cas9-sgRNA-A

    Plasmid
    #149369
    Purpose
    Expresses SpCas9 and sgRNA targeting the lenti-CDDR reporter
    Depositor
    Tarek Abbas
    Article
    Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592
    Inserts
    Cas9
    sgRNA-A
    Puromycin resistance
    Use
    CRISPR
    Tags
    3xFLAG, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Promoter
    CBh, PGK, and U6
    Available Since
    Nov. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    149369-plasmid-map-sequence-id-313973

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  7. Cas9-sgRNA-A+B

    Plasmid
    #149370
    Purpose
    Expresses SpCas9 and two sgRNA targeting the lenti-CDDR reporter at two distal sites
    Depositor
    Tarek Abbas
    Article
    Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592
    Inserts
    Cas9
    sgRNA-A
    sgRNA-B
    Puromycin resistance
    Use
    CRISPR
    Tags
    3xFLAG, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Promoter
    CBh, PGK, and U6
    Available Since
    Oct. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    149370-plasmid-map-sequence-id-315474

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  11. pShHELIX_sgRNA_entry (CJT32)

    Plasmid
    #181782
    Purpose
    Expresses ShHELIX containing a nicking I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    181782-plasmid-map-sequence-id-354808

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  17. pPEPZ-sgRNAclone

    Plasmid
    #141090
    Purpose
    This vector is designed for efficient cloning of sgRNAs by Golden Gate assembly. The sgRNA insertion leads to replacement of mCherry, resulting in loss of red color of E. coli colony.
    Depositor
    Jan-Willem Veening
    Article
    Liu et al Cell Host Microbe. 2021 Jan 13;29(1):107
    Insert
    mCherry
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    p3
    Available Since
    April 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    141090-plasmid-map-sequence-id-330475

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  19. pKS diaCas9_sgRNA

    Plasmid
    #74923
    Purpose
    Expresses Cas9 codon optimized for Phaeodactylum tricornutum and a sgRNA driven by a U6 promoter
    Depositor
    Per Winge
    Article
    Nymark et al Sci Rep. 2016 Apr 25;6:24951. doi: 10.10
    Inserts
    LHCF2 promoter
    diaCas9
    LHCF1 terminator
    U6 promoter
    sgRNA
    U6 3' region
    Use
    CRISPR
    Promoter
    Cas9 module and sgRNA module
    Available Since
    June 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    74923-plasmid-map-sequence-id-149703

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  20. p426_Cas9_gRNA-ARS1014a

    Plasmid
    #87396
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1014a sequence TTATGTGCGTATTGCTTTCA in yeast chromosome 10.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1014a
    Use
    CRISPR
    Promoter
    ADH1, pTyrosine
    Available Since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87396-plasmid-map-sequence-id-193439

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Showing: 561 - 580 of 16742 results