We narrowed to 17,485 results for: puro

1. pCRISPaint-TagBFP-PuroR

   Plasmid

   #80969

   Purpose

   CRISPaint gene tagging

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Insert

   TagBFP
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. qTAG-C-dTAG-Puro

   Plasmid

   #207727

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. MSCV CreERT2 puro

   Plasmid

   #22776

   Purpose

   Retroviral expression of tamoxifen-inducible Cre-ERT2

   Depositor

   Tyler Jacks

   Article

   Kumar et al Genes Dev. 2009 Dec 1;23(23):2700-4. doi

   Insert

   CreERT2
   

   Use

   Cre/Lox and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV

   Available since

   Jan. 21, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. Ki67-mNeonGreen-IRESpuro2

   Plasmid

   #183737

   Purpose

   Expresses Ki67-mNeonGreen

   Depositor

   Daniel Gerlich

   Article

   Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

   Insert

   MKI67 (MKI67 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO.1-puro-shNT

   Plasmid

   #109012

   Purpose

   short-hairpin knockdown of targeted gene

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   Non-targeting shRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6 promoter

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. TetO-Ascl1-puro

   Plasmid

   #97329

   Purpose

   Generating lentiviruses to express Ascl1 and puromycin resistance

   Depositor

   Marius Wernig

   Article

   Yang et al Nat Methods. 2017 May 15. doi: 10.1038/n

   Insert

   Ascl1 (Ascl1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   TET-ON

   Available since

   July 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSCV Puro-pGK:GFP

   Plasmid

   #68486

   Purpose

   Retrovirus expressing GFP driven by pGK promoter

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Inserts

   Puromycin Resistance
   GFP
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LTR and pGK

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. qTAG-N-Puro-dTAG

   Plasmid

   #207694

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-dTAG cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pLenti_gRNA -hMLHdn-Puro

   Plasmid

   #191105

   Purpose

   Backbone for lentiviral pegRNA library construction with hMLH1dn and Puro marker

   Depositor

   Hyongbum Kim

   Article

   Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

   Insert

   hMLH1dn
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   I31 silent mutation (ATA to ATC) to remove early …

   Promoter

   EF-1a

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEF1a-FB-dCas9-puro

    Plasmid

    #100547

    Purpose

    Expresses N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9 protein

    Depositor

    Jian Xu

    Article

    Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

    Inserts

    N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9
    puromycin
    

    Use

    CRISPR

    Tags

    FLAG, Biotin-acceptor-site (Avitag)

    Expression

    Mammalian

    Mutation

    Promoter

    Human EF1a

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-Puro-hGSDME

    Plasmid

    #154876

    Purpose

    Expresses hGSDME in mammalian cells

    Depositor

    Judy Lieberman

    Article

    Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

    Insert

    GSDME (GSDME Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDH-kz-CD20-Puro

    Plasmid

    #209759

    Purpose

    Lentiviral transfer plasmid to express the CDS of the human CD20 gene, MS4A1.

    Depositor

    Andrei Thomas-Tikhonenko

    Article

    Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

    Insert

    MS4A1 (MS4A1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCSIIpuro-EGF-ScNeo

    Plasmid

    #209893

    Purpose

    To monitor the status of EGF, the plasmid encodes a recombinant EGF fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    EGF-ScNeo (EGF Human)
    

    Use

    Lentiviral

    Tags

    GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCSIIpuro-HBEGF-ScNeo

    Plasmid

    #209894

    Purpose

    To monitor the status of HB-EGF, the plasmid encodes a recombinant HB-EGF fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    HBEGF-ScNeo (HBEGF Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen and mScarlet

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCSIIpuro-TGFa-ScNeo

    Plasmid

    #209895

    Purpose

    To monitor the status of TGFα, the plasmid encodes a recombinant TGFα fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    TGFa-ScNeo (TGFA Human)
    

    Use

    Lentiviral

    Tags

    GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. 5` CC: Puro – loxP – GFP-C

    Plasmid

    #219563

    Purpose

    5` circularization cassette.Used in combination with one of the 3' circularization cassettes to induce Cre-mediated circularizazion or inversion of a desired genomic region.

    Depositor

    Andrea Ventura

    Article

    Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586

    Insert

    hPGK-promoter_PuroR_2A_loxP_GFP-C
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    hPGK

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lenti-EF1alpha-ZsGreen1_Puro

    Plasmid

    #192661

    Purpose

    3rd generation lenti vector encoding ZsGreen1 with 2A Puro resistance marker

    Depositor

    Jeffrey Miner

    Insert

    ZsGreen1
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lenti-entry-puro

    Plasmid

    #85745

    Purpose

    Lentiviral vector for generating multiple sgRNAs carrying plasmids by Golden Gate Assembly.

    Depositor

    Qin Yan

    Article

    Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENTR-L1-PGK-PuroTK-L2

    Plasmid

    #141005

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    PuroTK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pIRES-EGFP-puro

    Plasmid

    #45567

    Depositor

    Michael McVoy

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    IRES-EGFP-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    Phcmv (strong human cytomegalovirus immediate ear…

    Available since

    June 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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