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LentiCRISPRv2puro-Ptgs2_B6

Plasmid

#170300

Purpose

A knock-out vector for the mouse Ptgs2 in C57BL6

Depositor

Michiyuki Matsuda

Article

Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

Insert

A gRNA targeting the mouse tyrosinase gene.

Use

CRISPR and Lentiviral

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pINDUCER21-HOXA9

Plasmid

#51302

Purpose

Expression of HOXA9 after doxycycline administration

Depositor

George Daley

Article

Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

Insert

homeobox A9 (HOXA9 Human)

Use

Lentiviral

Tags

n/a

Mutation

n/a

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2hyg-Ptgs1

Plasmid

#170299

Purpose

A knock-out vector for the mouse Ptgs1

Depositor

Michiyuki Matsuda

Article

Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

Insert

A gRNA targeting the mouse Ptgs1 gene.

Use

CRISPR and Lentiviral

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-PRDM1

Plasmid

#222924

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of PRDM1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

PRDM1 (PRDM1 Human)

Expression

Mammalian

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCambia2300 - ProRUBY:RUBY-Citrine

Plasmid

#128796

Purpose

Fluorescent reporter for subcellular localisation and expression analysis of RUBY

Depositor

George Haughn

Article

Sola et al Plant Cell. 2019 Apr;31(4):809-831. doi:

Insert

RUBY PARTICLES IN MUCILAGE (AT1G19900 Mustard Weed)

Tags

Citrine

Expression

Plant

Promoter

ProRUBY

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pSMP-EZH2_2

Plasmid

#36388

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

EZH2 (EZH2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

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pSMP-EZH2_3

Plasmid

#36389

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

EZH2 (EZH2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pPB-cT3G-cERH-ZNF281

Plasmid

#222923

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of ZNF281

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

ZNF281 (ZNF281 Human)

Expression

Mammalian

Available Since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FLAG-HA-DGCR8

Plasmid

#106251

Purpose

Express DGCR8 in mammalian cells

Depositor

Joshua Mendell

Article

Chang et al Genome Res. 2015 Sep;25(9):1401-9. doi:

Insert

DiGeorge Syndrome Critical Region 8 (DGCR8 Human)

Tags

FLAG-HA

Expression

Mammalian

Promoter

CMV

Available Since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

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pNG1256

Plasmid

#149710

Purpose

Expresses RpoA, RpoB, RpoC-9His and RpoZ from Bacillus subtilis to produce core RNAP complex

Depositor

Peter Lewis

Article

Newing et al Nat Commun. 2020 Dec 18;11(1):6420. doi:

Insert

rpoZ (rpoZ )

Expression

Bacterial

Promoter

T7

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pHDR2_DAZL-T2A-mGreenLantern-PuroTK

Plasmid

#222917

Purpose

Homology directed repair template for knocking in mGreenLantern reporter to DAZL.

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

mGreenLantern

Use

CRISPR

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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gRNA_DNMT3a-T1

Plasmid

#41821

Purpose

Expresses a guide RNA (gRNA) to target DNMT3a (T1 target sequence) for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

gRNA_DNMT3a-T1 (DNMT3A Human)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pCS2+PhyBΔCreC

Plasmid

#131781

Purpose

Mammalian, Zebrafish, or Xenopus expression of PhyBΔCreC, one half of the CreLite system.

Depositor

George Eisenhoffer

Article

Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

Insert

PhyBCreC

Use

Cre/Lox; Zebrafish expression

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pSMP-EZH1_1

Plasmid

#36359

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

EZH1 (EZH1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pMS_375

Plasmid

#182132

Purpose

expresses retron RNA, ec.86 Reverse Transcriptase, CspRecT SSAP, and mutL* to create a specific rifampicin resistance mutation while inhibiting MMR

Depositor

George Church

Article

Schubert et al Proc Natl Acad Sci U S A. 2021 May 4;118

Inserts

retron RNA
ec.86 RT
CspRecT

Mutation

incorporated rifampicin-resistance-conferring don…

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pSMP-Bmi1_1

Plasmid

#36352

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Bmi1 (BMI1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

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pSMP-Dot1L_1

Plasmid

#36339

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Dot1L (DOT1L Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
gRNA_DNMT3a-T2

Plasmid

#41822

Purpose

Expresses a guide RNA (gRNA) to target DNMT3a (T2 target sequence) for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

gRNA_DNMT3a-T2 (DNMT3A Human)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only
gRNA_DNMT3b

Plasmid

#41823

Purpose

Expresses a guide RNA (gRNA) to target DNMT3b for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

gRNA_DNMT3b (DNMT3B Human)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only
pSMP-Bmi1_3

Plasmid

#36354

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Bmi1 (BMI1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only