We narrowed to 31,240 results for: TES
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Plasmid#92319PurposeExpression of NTerm 6xHis tagged mRFP mutant K15TAG for ncAA incoporation using the p70a promoter and UTR3DepositorInsertOptimized NTerm 6xHis tagged monomeric RFP (mRFP)
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialPromoterp70aAvailable SinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-Nterm 6xHis mRFP K15TAG-T500
Plasmid#92318PurposeExpression of NTerm 6xHis tagged mRFP mutant K15TAG for ncAA incoporation using the p70a promoter and UTR1DepositorInsertOptimized NTerm 6xHis tagged monomeric RFP (mRFP)
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialPromoterp70aAvailable SinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-NTerm 6xHis-zmADH V86TAG-T500
Plasmid#92230PurposeExpression of zmADH mutant V86TAG for ncAA incoporation using the p70a promoter and UTR1DepositorInsertNTerm 6xHis Tagged zmADH
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationResidue V86 mutated into TAG (Amber) stop codon f…Promoterp70aAvailable SinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
10xHis-ataxin-3 *SIM-HA
Plasmid#89983PurposeExpresses His- and HA-tagged ataxin-3 with a mutation in the SUMO-interacting motifDepositorInsertataxin-3 (ATXN3 Human)
Tags10xHis and HAExpressionMammalianMutationmutated aa 162IFVV to 162AFAAAvailable SinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-ataxin-3 *SIM
Plasmid#89979PurposeExpresses GFP-tagged ataxin-3 with a mutation in the SUMO-interacting motifDepositorAvailable SinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-NSF/T646A
Plasmid#74937PurposeMammalian expression of human NSF mutant T646A (mutation in the D2 domain of the protein)DepositorInsertNSF (NSF Human)
TagsFLAGExpressionMammalianMutationT646A (mutation in the D2 domain of the protein)PromoterCMVAvailable SinceApril 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-NSF/T645A
Plasmid#74936PurposeMammalian expression of human NSF mutant T645A (mutation in the D2 domain of the protein)DepositorInsertNSF (NSF Human)
TagsFLAGExpressionMammalianMutationT645A (mutation in the D2 domain of the protein)PromoterCMVAvailable SinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-CD4-YEML-myc
Plasmid#58538PurposeExpresses human CD4 with the IFITM3 endocytosis motif (YEML) and a C-terminal myc tag in mammalian cells.DepositorInsertCD4 (CD4 Human)
TagsIFITM3 endocytosis Tyrosine-Glutamate-Methionine-…ExpressionMammalianMutationAdded C-terminal Tyrosine-Glutamate-Methionine-Le…Available SinceSept. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET21a-MKP7_K62R_153
Plasmid#37795DepositorInsertMitogen-activated protein kinase phosphatase 7 (DUSP16 Human)
TagsHisExpressionBacterialMutationchanged Lysnie 62 to Arginine and deleted amino a…Available SinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET21a-MKP7_K52R_153
Plasmid#37793DepositorInsertMitogen-activated protein kinase phosphatase 7 (DUSP16 Human)
TagsHisExpressionBacterialMutationchanged Lysnie 52 to Arginine and deleted amino a…Available SinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
HNF4A_P2-198
Plasmid#31065DepositorInsertHNF4A P2 promoter (HNF4A Human)
UseLuciferaseExpressionMammalianMutation-198 to -1 of P2 HNF4A promoterAvailable SinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
PPRE X3-TK-luc
Plasmid#1015PurposePPAR reporter. 3xDR1 sites upstream of a luciferase reporter.DepositorInsertPPRE
UseLuciferaseExpressionMammalianAvailable SinceNov. 3, 2005AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 - hSRSF6
Plasmid#120419Purposeoverexpression of human SRSF6DepositorAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
HT-mGold
Plasmid#183986PurposeExpresses fluorescent HaloTag7 fusion protein in mammalian cells in the whole cell.DepositorInsertHaloTag-mGold
TagsHaloTag, mGoldExpressionMammalianAvailable SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFA104.PI.eGFP.WPRE.bGH
Plasmid#100896PurposeAAV expression of EGFP in astrocytesDepositorHas ServiceAAV5InsertEGFP
UseAAVExpressionMammalianPromoterGFA104 (GFAP promoter)Available SinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTF101.3
Plasmid#134772PurposeA binary plasmid containing double CaMV 35S promoter to drive bialophos resistance gene (bar) for plant transformation.DepositorTypeEmpty backboneExpressionPlantAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCiVSP-SD64TF
Plasmid#80332PurposeExpresses Ciona VSP in Xenopus OocyteDepositorInsertCiona intestinalis voltage-sensing phosphatase (vsp Ciona intestinalis)
UseXenopus oocyte expressionPromoterSP6Available SinceOct. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
bRA66
Plasmid#100952PurposeGAL1 driven Cas9. gRNA can be cloned into BplI sites.DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT7-pelB
Plasmid#157740Purposeempty vector; N-terminal PelB signal sequence; potential C-terminal His6; T7 promoter; like pET-20b, but additionally avoids added residues in final protein since cloning uses BseRI restriction sitesDepositorTypeEmpty backboneTagsPelB signal sequence and possible His6-tag (adds …ExpressionBacterialPromoterT7Available SinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-FRT
Plasmid#183241Purposeanhydrotetracycline (aTC) inducible sgRNA that targets FRT scar sites and arabinose inducible lambda RedDepositorInsertsgRNA-FRT
ExpressionBacterialPromoterP-tetAvailable SinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRVV661
Plasmid#108282PurposeDonor vector for creation of PhiC31 attP-flanked platforms for cassette exchange using CRISPR/Cas9DepositorInsertPhiC31 attP sites
UseCRISPRAvailable SinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRVV669
Plasmid#108283PurposeDonor vector for creation of PhiC31 attP-flanked platforms for cassette exchange using CRISPR/Cas9DepositorInsertPhiC31 attP sites
UseCRISPRAvailable SinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pREP
Plasmid#51491PurposepMDC32 with Rep/RepA coding sequence from the mild bean yellow dwarf virusDepositorInsertRep/RepA
UsePlant expression t-dnaMutationnonePromoter2x35SAvailable SinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
bRA77
Plasmid#100953PurposeGAL1 driven Cas9. gRNA can be cloned into BplI sites.DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pREPK219H
Plasmid#51492PurposepREP with a mutation in the ATPase domain (K219H). This is predicted to result in loss of geminivirus replication.DepositorInsertRep/RepA
UsePlant expression t-dnaMutationchanged lysine 219 to histidinePromoter2x35SAvailable SinceMay 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 - mSRSF6
Plasmid#120420Purposeoverexpression of mouse Srsf6DepositorAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pES133
Plasmid#127552PurposeVariant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F5-tetA-F5 cassette orthogonal to FRT-KanR-FRT in pKD13DepositorInsertF5-flanked tetracycline resistance cassette
ExpressionBacterialMutationMultiple SNPs in tetA (originally from pBR322) to…Available SinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
Sup35 ScNM A230C His7
Plasmid#15598DepositorInsertSup35 NM (SUP35 Budding Yeast)
Tags7xHisExpressionBacterialMutationA230C. Alanine 230 mutated to cysteine.Available SinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCS2+methylated-reporter
Plasmid#164491PurposeReporter to test methylated mRNA stability, for mRNA injectionDepositorInsertA-less CDS, HA tag, 12x m6A motif 3'UTR
UseProduction of mrna for zebrafish injection.TagsHA tagPromoterSP6Available SinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLSLGUS
Plasmid#51517PurposepLSL with GUS coding sequence followed by 3 kb of filler sequence Rep is not included on this plasmidDepositorInsertsGUS
3kb filler sequence
UsePlant t-dna plasmidTagsNLS and flagPromotervirion-sense LIR and 2x35SAvailable SinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only