We narrowed to 34,882 results for: cas

1. cr3Dd

   Plasmid

   #184862

   Purpose

   cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and a species-specific recombination system

   Depositor

   Patrick Sobetzko

   Article

   Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

   Insert

   Cas9, bet, exo
   

   Use

   Tags

   Cas9 ssrA degradation tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. MLM3613

   Plasmid

   #42251

   Purpose

   Expresses Cas9 nuclease (Streptococcus pyogenes) from CMV and T7 promoters

   Depositor

   Keith Joung

   Article

   Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

   Insert

   Streptococcus pyogenes Cas9
   

   Use

   CRISPR; Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Jan. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSB-BbsI-sgRNA

   Plasmid

   #203359

   Purpose

   Contains BbsI cut sites for placement of user-specified sgRNA target sequences with Sleeping Beauty backbone

   Depositor

   Jonathan Brunger

   Article

   Gil et al Bio Protoc. 2024 Jan 5;14(1):e4913. doi:

   Insert

   BbsI Cut Sites
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSL0283 (pTnsABC)

   Plasmid

   #130633

   Purpose

   Expresses V. cholerae CAST TnsA, TnsB, and TnsC from a T7 promoter.

   Depositor

   Samuel H. Sternberg

   Article

   Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

   Insert

   VchTnsA, VchTnsB, VchTnsC
   

   Use

   CRISPR; Transposon

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMT423

   Plasmid

   #139382

   Purpose

   Constitutively expressed dCas9, LacI and TetR, pNBU2 backbone, AmpR, TetR

   Depositor

   Christopher Voigt

   Article

   Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

   Insert

   Constitutively expressed dCas9, LacI and TetR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   P1

   Available since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pWJ66

   Plasmid

   #46570

   Purpose

   Same as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9)

   Depositor

   Luciano Marraffini

   Article

   Bikard et al Nucleic Acids Res. 2013 Jun 12.

   Inserts

   tracrRNA
   dcas9-w
   CRISPR array
   

   Use

   CRISPR

   Tags

   rpoZ

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   Available since

   Aug. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTXRE5-Z

   Plasmid

   #112509

   Purpose

   Reporter plasmid, responsive to activated Ah receptor/Arnt

   Depositor

   Charles Miller

   Article

   Miller CA et al Toxicol Appl Pharmacol. 1999 Nov 1;160(3

   Insert

   RW95-3 with 5x xenobiotic response elements (lacZ E.coli, Synthetic)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   5 XREs+ minimal Cytochrome C

   Available since

   July 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCFD2-dU6:2gRNA

   Plasmid

   #49409

   Purpose

   expression of gRNA under control of the Drosophila U6:2 promoter

   Depositor

   Simon Bullock

   Article

   Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

   Insert

   dU6-2:gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   dU6-2

   Available since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCFD1-dU6:1gRNA

   Plasmid

   #49408

   Purpose

   expression of gRNA under control of the Drosophila U6:1 promoter

   Depositor

   Simon Bullock

   Article

   Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

   Insert

   dU6-1:gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   dU6-1

   Available since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSIBR004

    Plasmid

    #177667

    Purpose

    SIBR Intron 4 (Int4) interrupts the CDS of FnCas12a

    Depositor

    John van der Oost

    Article

    Patinios et al Nucleic Acids Res. 2021 Oct 6. pii: 6382

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRR-PR-5Z

    Plasmid

    #23057

    Depositor

    Charles Miller

    Article

    Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

    Insert

    PR (PGR Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pChimera

    Plasmid

    #61476

    Purpose

    Vector for conventional cloning that contains AtU6-26 promoter and sgRNA backbone

    Depositor

    Holger Puchta

    Article

    Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

    Insert

    U6-26:sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    U6-26

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. cr3Vn

    Plasmid

    #184861

    Purpose

    cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an V. natriegens-specific recombination system

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    Cas9, gam, bet, exo
    

    Use

    Tags

    Cas9 ssrA degradation tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSIBR003

    Plasmid

    #177666

    Purpose

    SIBR Intron 3 (Int3) interrupts the CDS of FnCas12a

    Depositor

    John van der Oost

    Article

    Patinios et al Nucleic Acids Res. 2021 Oct 6. pii: 6382

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pWJ68

    Plasmid

    #46571

    Purpose

    Same as pdCas9, but with the w-dCas9 fusion (w is fused at the N-terminal end of dCas9)

    Depositor

    Luciano Marraffini

    Article

    Bikard et al Nucleic Acids Res. 2013 Jun 12.

    Inserts

    tracrRNA
    w-dcas9
    CRISPR array
    

    Use

    CRISPR

    Tags

    rpoZ

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    Available since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. cr3Ec

    Plasmid

    #184860

    Purpose

    cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an E.coli-specific recombination system

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    Cas9, gam, bet, exo
    

    Use

    Tags

    Cas9 ssrA degradation tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MLM3639

    Plasmid

    #42252

    Purpose

    Cas9 expression vector

    Depositor

    Keith Joung

    Article

    Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    Streptococcus pyogenes Cas9-3X Flag
    

    Use

    CRISPR; Zebrafish expression

    Tags

    3X Flag

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJK506

    Plasmid

    #155382

    Purpose

    dCas12a (D917A) + PA4-mVenus. (removed crRNAs)

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas12a (F. novicida)
    PA4-mVenus
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D917A (nuclease-deactivating)

    Promoter

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDrop

    Plasmid

    #184872

    Purpose

    Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for in-vivo excision of transferred DNA for homologous recombination and counter-selection for successful integration.

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    lacZalpha,ccdB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pCBC-DT1T2.2

    Plasmid

    #134752

    Purpose

    PCR template for sgRNA cloning

    Depositor

    Qi-Jun Chen

    Article

    Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

    Insert

    sgRNA-AtU6_29p
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge