We narrowed to 19,051 results for: Tec;

1. pOCC102

   Plasmid

   #118883

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C protease

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-stop-HindIII cassette
   

   Tags

   HIS6-MBP, cleavable with 3C protease

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAcHELIX-sgRNA_entry (CJT94)

   Plasmid

   #181783

   Purpose

   Expresses AcHELIX containing a nicking I-AniI fusion to AcTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   nAniI-AcTnsB, AcTnsC, AcTniQ, AcCas12k
   

   Expression

   Bacterial

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pShuttle-CMV-iRFP

   Plasmid

   #31856

   Purpose

   a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging

   Depositor

   Vladislav Verkhusha

   Article

   Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

   Insert

   iRFP
   

   Use

   Adenoviral

   Expression

   Mammalian

   Mutation

   T2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198…

   Promoter

   CMV

   Available Since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAVS1-Puro cTnT FUCCI

   Plasmid

   #136935

   Purpose

   donor plasmid for cardiac-specific FUCCI expression in human cells

   Depositor

   Xiaoping Bao

   Article

   Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

   Insert

   Troponin T promoter
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   cTnT

   Available Since

   April 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. sgLenti-orthogonal

   Plasmid

   #105997

   Purpose

   Dual Streptococcus pyogenes and Staphylococcus aureus sgRNA expression vector

   Depositor

   Mike McManus

   Article

   Boettcher et al Nat Biotechnol. 2018 Jan 15. pii: nbt.40

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral; S.pyogenes sgrna expressio…

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. Cytosolic FAK biosensor

   Plasmid

   #78300

   Purpose

   FRET biosensor. Visualization of cytosolic FAK activity in live cells

   Depositor

   Yingxiao Wang

   Article

   Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

   Insert

   Cytosolic FAK biosensor
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBK-VRER-BE3

   Plasmid

   #85173

   Purpose

   Expresses VRER-BE3 in mammalian cells

   Depositor

   David Liu

   Article

   Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Insert

   VRER-BE3
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGWB418

   Plasmid

   #74812

   Purpose

   Gateway cloning compatible binary vector for N-terminal fusion with 4xMyc (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   Sept. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAS1yl

   Plasmid

   #73226

   Purpose

   Constitutive expression of Cas9 and sgRNA in Yarrowia lipolytica cells

   Depositor

   Sheng Yang

   Article

   Gao et al J Ind Microbiol Biotechnol. 2016 Aug;43(

   Inserts

   Cas9
   sgRNA
   

   Use

   CRISPR; In y. lipolytica

   Expression

   Bacterial

   Promoter

   unknown

   Available Since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBK-VQR-BE3

    Plasmid

    #85171

    Purpose

    Expresses VQR-BE3 in mammalian cells

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    VQR-BE3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. 3XFlagNICD4

    Plasmid

    #20186

    Depositor

    Raphael Kopan

    Article

    Ong et al J Biol Chem. 2006 Feb 24. 281(8):5106-19

    Insert

    Notch 4 Intracellular Domain (Notch4 Mouse)
    

    Tags

    3X Flag

    Expression

    Mammalian

    Mutation

    Contains murine Notch 4 Intracellular Domain (Val…

    Promoter

    CMV

    Available Since

    Dec. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pGWB515

    Plasmid

    #74857

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with 3xHA (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGWB442

    Plasmid

    #74828

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with eYFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMXs-ms-Sox3

    Plasmid

    #50779

    Purpose

    retroviral expression of mouse Sox3

    Depositor

    Shinya Yamanaka

    Article

    Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

    Insert

    Sox3 (Sox3 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. piGECI-N1

    Plasmid

    #160421

    Purpose

    Cytoplasmic expression of a near-infrared genetically encoded calcium indicator iGECI

    Depositor

    Vladislav Verkhusha

    Article

    Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

    Insert

    iGECI
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. ND4.2-Left TALE-G1397-N-dead-mCherry

    Plasmid

    #179683

    Purpose

    Expression of base editor in mammalian cells and for cell sorting

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (E1347A)_1xUGI_P2A_mCherry_ATP5B 3'UTR
    

    Expression

    Mammalian

    Mutation

    E1347A

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. LCV2 control

    Plasmid

    #217443

    Purpose

    Lentiviral vector expressing Cas9 without a targeting sgRNA

    Depositor

    Jarrod Johnson

    Article

    Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. HBT-pcoCas9

    Plasmid

    #52254

    Purpose

    transient expression of pcoCas9 gene in plant cells

    Depositor

    Jen Sheen

    Article

    Li et al Nat Biotechnol. 2013 Aug;31(8):688-91. d

    Insert

    pcoCas9
    

    Use

    CRISPR; Plant expression

    Tags

    FLAG and NLS

    Promoter

    hybrid constitutive promoter 35SPPDK

    Available Since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC19/ER-mChe

    Plasmid

    #183163

    Purpose

    Construct for transient expression of endoplasmic reticulum-targeted mCherry in plants.

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Insert

    ER-mCherry
    

    Tags

    25 aa ER signal of Glycine soja kunitz-type tryps…

    Expression

    Plant

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEF1alpha-rtTA-Antares

    Plasmid

    #197587

    Purpose

    Expresses TET-On reverse transactivator labeled with Antares

    Depositor

    Mikhail Shapiro

    Article

    Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    rtTA-P2A-Antares
    

    Tags

    Antares

    Expression

    Mammalian

    Promoter

    EF-1alpha

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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