We narrowed to 6,229 results for: cas9 expression plasmid

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pJOG251

Plasmid

#80583

Purpose

recipient plasmid [multiplexing, activator]; p35S:Cas9 D10A/N863A-Hax3CT, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Inserts

pnos:PAT-tnos
p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
BsaI-ccdB_CmR-BsaI

Use

CRISPR

Expression

Plant

Mutation

D10A

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pJOG252

Plasmid

#80584

Purpose

recipient plasmid [single activator]; p35S:Cas9 D10A/N863A-Hax3CT, nptII

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Inserts

pnos:nptII-tnos
p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
pAtU6-BpiI-ccdB_CmR-BpiI_sgRNA_scaffold

Use

CRISPR

Expression

Plant

Mutation

D10A

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pJOG253

Plasmid

#80585

Purpose

recipient plasmid [single activator]; p35S:Cas9 D10A/N863A-Hax3CT, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Inserts

pnos:PAT-tnos
p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
pAtU6-BpiI-ccdB_CmR-BpiI_sgRNA_scaffold

Use

CRISPR

Expression

Plant

Mutation

D10A

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pX330A-nBRD4/PITCh

Plasmid

#91794

Purpose

PITCh sgRNA, N-terminal BRD4 sgRNA, and Cas9 expressing plasmid for use with the dTAG knock-in system and BRD4

Depositor

James Bradner, Behnam Nabet

Article

Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

Insert

SpCas9

Use

CRISPR

Expression

Mammalian

Promoter

chicken β-actin promoter

Available Since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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pET28b-zDjCas13d-His

Plasmid

#234481

Purpose

Plasmid for bacterial expression and purification of DjCas13d protein (zebrafish codon-optimized)

Depositor

Miguel Angel Moreno-Mateos

Article

Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

Insert

DjCas13d

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Available Since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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SPACE (pRZ1813)

Plasmid

#140242

Purpose

CMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-UGI-UGI-P2A-EGFP

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

SPACE

Expression

Mammalian

Mutation

V82G in TadA*, D10A in Cas9, R187W in pmCDA1

Promoter

CMV

Available Since

June 3, 2020

Availability

Academic Institutions and Nonprofits only

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pML107-NET1

Plasmid

#232893

Purpose

Plasmid expressing Cas9 and gRNA GTGCCACTAATGGATCCATG which targets the NET1 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

NET1 gRNA (NET1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-VRG4

Plasmid

#232899

Purpose

Plasmid expressing Cas9 and gRNA TCGCATAGCCCAGTATACGT which targets the VRG4 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

VRG4 gRNA (VRG4 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pML104-HIS3-3

Plasmid

#232882

Purpose

Plasmid expressing Cas9 and gRNA AAACCTTTTTACTCCACGCA which targets the HIS3 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

HIS3 gRNA (HIS3 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pML104-HIS3-2

Plasmid

#232881

Purpose

Plasmid expressing Cas9 and gRNA CCAAGTTCGACAACTGCGTA which targets the HIS3 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

HIS3 gRNA (HIS3 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pWZ157

Plasmid

#163639

Purpose

pcn-1>PCN-1::GFP expression plasmid for CRISPR/Cas9 integration at ttTi4348 site on chromosome I

Depositor

David Matus

Article

Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1

Insert

pcn-1 (pcn-1 Nematode)

Use

CRISPR

Tags

GFP

Expression

Worm

Promoter

pcn-1

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pWZ186

Plasmid

#163641

Purpose

rps-27>DHB::2xmKate2 expression plasmid for CRISPR/Cas9 integration at ttTi4348 site on chromosome I

Depositor

David Matus

Article

Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1

Insert

DHB:2xmKate2::3xHA

Use

CRISPR

Tags

2xmKate2

Expression

Worm

Promoter

rps-27

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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BE3-P2A-EGFP (pJUL977)

Plasmid

#123612

Purpose

CAG promoter expression plasmid for rAPOBEC1-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP.

Depositor

Keith Joung

Article

Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

Insert

BE3-P2A-EGFP

Expression

Mammalian

Mutation

D10A in SpCas9

Promoter

CAG

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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Biomolecular FeedBack (FB)

Plasmid

#109391

Purpose

This construct encodes for two units. The first contains a constitutively expressed dCas9 protein. The second contains an sgRNA cassette under the control of the burden-responsive htpG1 promoter.

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Insert

pJ23100mut-dCas9-T- phtpG1-sgRNA

Expression

Bacterial

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pRS143

Plasmid

#122377

Purpose

CRISPRi plasmid for use in Candida albicans. Contains dCas9 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)

Depositor

Rebecca Shapiro

Article

Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-COX8A

Plasmid

#227308

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of COX8A for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of COX8A (COX8A Human)

Use

CRISPR

Expression

Mammalian

Promoter

U67

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-ACTB

Plasmid

#207748

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of ACTB for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of ACTB (ACTB Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-pCbh-SpRY[C-term]-gRNAentry (CA20)

Plasmid

#197511

Purpose

Cbh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpRY and BsmBI entry cassette to clone SpCas9 gRNA spacer

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

Insert

AAV-[ITR]-pCbh-BPNLS-NpuC-SpRY[C-term]-BPNLS-gRNA[BsmBI]-pU6-[ITR]

Use

AAV and CRISPR

Tags

BPNLS and BPNLS-NpuC(intein)

Mutation

C-terminal mutations of SpRY(L1111R/D1135L/S1136W…

Promoter

Cbh

Available Since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGG184

Plasmid

#165604

Purpose

Reporter plasmid for B1H-dependent expression of the HIS3/GFP operon with a single hEGFP protospacer: PS1(upstream of promoter with 'CAGCG' PAM)-lac-HIS3 and GFP

Depositor

Marcus Noyes

Article

Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

Insert

hEGFP protospacer ('CAGCG' PAM) upstream of the HIS3/GFP promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

'CAGCG' PAM replacing the 'CGGCG&#…

Promoter

lac

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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psPAX2-D64V-NC-MS2

Plasmid

#122944

Purpose

MS2 coat protein-modified lentiviral packaging plasmid for packaging mRNA with an MS2 aptamer into lentivirus-like particles

Depositor

Baisong Lu

Article

Lu et al Nucleic Acids Res. 2019 Feb 13. pii: 531

Insert

NC-MCP (MS2 coat protein) fusion protein in Gag

Use

Lentiviral

Expression

Mammalian

Mutation

D64V

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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