We narrowed to 66,557 results for: des.1
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pAAV.hSynapsin.SF-iGluSnFR.S72A (AAV1)
Viral Prep#106176-AAV1PurposeReady-to-use AAV1 particles produced from pAAV.hSynapsin.SF-iGluSnFR.S72A (#106176). In addition to the viral particles, you will also receive purified pAAV.hSynapsin.SF-iGluSnFR.S72A plasmid DNA. hSynapsin driven SF-iGluSnFR.S72A, glutamate sensor. The S72A mutant has lower affinity for glutamate. Slower on-rate and faster off-rate. Enhanced temporal resolution and spatial resolution. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable SinceFeb. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1_TREtight-GEM2-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1
Plasmid#197061PurposeDonor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM2-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)DepositorInsertsEncapsulin
Adaptor Protein
UseCRISPRTagsFRB and HaloTagExpressionMammalianMutationCodon-optimised for human cell expressionAvailable SinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-LSSmGFP
Plasmid#186543PurposeLSSmGFP fluorescent protein (ex/em = 400/510 nm): cytosolic expression in mammalian cellsDepositorInsertLSSmGFP
ExpressionMammalianMutationhfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206KPromoterCMVAvailable SinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FRB-LgBiT
Plasmid#182650PurposeFRB-LgBiTDepositorInsertFRB-LgBiT fusion
TagsLgBiTExpressionMammalianPromoterCMVAvailable SinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
AbVec2.1-mIglc2
Plasmid#127156PurposeExpression of immunoglobulin light chains in mammalian cells, mouse (C57BL/6), lambda 2 constant regionDepositorAvailable SinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.D2-NP
Plasmid#134366PurposeNanoluc complementation assay. Expression of dopamine receptor D2 fused at C terminus with Natural peptide (NP) of NanoLuc. Addition of the signal sequence and Flag epitope at N terminus of D2.DepositorInsertD2-NP (DRD2 Human)
TagsFlag, natural peptide of nanoluciferase, and sign…ExpressionBacterial and MammalianPromoterT7Available SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Beta1
Plasmid#140987PurposeEncodes a Gbeta subunit (GNB1) as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGBeta1 (GNB1 Human)
ExpressionMammalianMutationContains an N-terminal human rhinovirus (HRV) 3C …PromoterCMVAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)- e18
Plasmid#131670PurposeExpresses an engineered variant of RAD18 ("e18") that stimulates CRISPR mediated HDR.DepositorInserte18 (engineered RAD18 with SAP domain deleted) (RAD18 Human)
TagsFLAG and HAExpressionMammalianPromoterCMVAvailable SinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+CMV-Ins-GLuc
Plasmid#89929PurposeExpresses Insulin-GLuc from the CMV promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.DepositorInserthuman insulin-Gaussia-Luciferase (INS Human, Synthetic)
ExpressionMammalianMutationDNA sequence for Gaussia luciferase was humanized…PromoterCMVAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
aCat TS WT (1-697 mTFP1-TSmod-Venus 698-907)
Plasmid#101299PurposeFRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.DepositorInsertalpha-Catenin
ExpressionMammalianMutationContains amino acids 1-698 and 698-906 of alpha-C…Available SinceJune 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CD40L-mgSrtA
Plasmid#125795Purposefor co-expression of CD40L (CD40 ligand) and mgSrtA on cell membrane. For proof-of-concept monitoring and labeling of CD40 positive cells.DepositorInsertCD40L-mgSrtA
ExpressionMammalianAvailable SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR2.1-InC
Plasmid#83959PurposeContains a synthetic insert to serve as internal control DNA template for a Salmonella Real-Time PCR assay.DepositorInsertInternal Control sequence
ExpressionBacterialAvailable SinceNov. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_PsChR2_EYFP
Plasmid#69057PurposeHigh-efficiency blue-shifted cation channelrhodopsinDepositorInsertPsChR2
TagsEYFPExpressionMammalianPromoterCMVAvailable SinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB2-SA(1)-T2A-LexA::QFAD-Hsp70
Plasmid#62948PurposeCreating LexA::QF drivers from MiMIC lines with inserts into coding introns in Phase 1DepositorInsertT2A-LexA::QFAD-Hsp70
Available SinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB2-SA(1)-T2A-Gal4-Hsp70
Plasmid#62897PurposeCreating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 1.DepositorInsertT2A-Gal4-Hsp70
Available SinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR-53BP1 1-1972 WT
Plasmid#53446PurposeRecombinational donor/master vectorDepositorInsert53BP1 (TP53BP1 Human)
UseGateway CloningMutationsiRNA resistant and mutations A128V, T720A, A1347…Available SinceApril 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1-CHRNA7-mCherry
Plasmid#62632Purposemammalian expression of human Alpha7 (CHRNA7) with mCherry fused into M3-M4 loopDepositorInserthAlpha7 (CHRNA7 Human)
ExpressionMammalianMutationmCherry inserted into M3-M4 loopPromoterCMVAvailable SinceMarch 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX330 Ctnnb1.1
Plasmid#59911PurposepX330 backbone expressing sgRNA targeting Ctnnb1 to edit mouse beta-Catenin. Expresses Cas9 from CBh promoterDepositorAvailable SinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
CMVp-dCas9-3xNLS-VP64 (Construct 1)
Plasmid#55195PurposeExpresses taCas9 in Mammalian cells for transactivating endogenous and synthetic promoters. The backbone is a lentiviral vector.DepositorInsertdCas9
UseCRISPR and Synthetic BiologyTagsVP64ExpressionMammalianPromoterCMV/hUBCAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only
