We narrowed to 33,713 results for: Eng
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Plasmid#48236PurposeDual expression construct expressing both dCas9VP48 and sgRNA from separate promotersDepositorInsertdCas9VP48
UseCRISPRTagsHA-tag and VP48ExpressionMammalianMutationD10A H840A (catalytically inactive)Available SinceSept. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
RSV/hGATA3
Plasmid#83814PurposeMammalian expression of human GATA3DepositorAvailable SinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag HBx (G124L, I127A)
Plasmid#42598DepositorInsertHepatitis B virus protein X (G124L, I127A)
TagsFlagExpressionMammalianMutationG124L I127AAvailable SinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEM-NLS-Cre-2A-mCherry-FKF
Plasmid#119868PurposeDonor cassette containing NLS-tagged Cre, a Thosea asigna virus peptide (2A), mCherry and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertNLS-Cre, 2A, mCherry and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
TagsmCherryExpressionBacterialAvailable SinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
PUC19_C_tag
Plasmid#186659PurposeEmpty donor plasmid for C-terminal tag of 3xFlag-3xHA.DepositorInsertC-terminal 3xFlag-3xHA Tag
Tags3xFlag-3xHAExpressionInsectAvailable SinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFJ150-GFP(dpiRNA)::CDK-1
Plasmid#107938Purposecdk-1p::GFP(dpiRNA)::cdk-1::cdk-1 3'UTR construct used for MosSCI in nematode. GFP is optimized by removing all piRNA targeting sites to allow germline expression.DepositorAvailable SinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAC91-pmax-dCas9VP64
Plasmid#48223PurposedCas9VP64 on pmax expression vectorDepositorInsertdCas9(D10A;H840A) fusion with VP64 activation domain
UseCRISPRTagsHA Tag and VP64ExpressionMammalianMutationD10A;H840A (catalytically inactive)PromoterCAGGSAvailable SinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUB6-CDC34-HA (pRKB261)
Plasmid#99145PurposeMammalian expression construct to express HA-tagged CDC34DepositorAvailable SinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPbcsx28
Plasmid#89909PurposeExpresses csx28 from P. buccae ATCC 33574 in bacteria. Expression driven from the Lac promoterDepositorInsertcsx28
ExpressionBacterialPromoterLacAvailable SinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIMVAu1
Plasmid#98301PurposeEnhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of galactose-inducible promoters; integrated into genome HMG2 locus; overexpressing HMG2 K6R.DepositorInsertHMG2(-309,-153)-HIS3-TEFM1ACS2>TACS2-PGAL2> EfmvaE >TEBS1-PGAL7>HMG2K6A(1,292)
ExpressionYeastAvailable SinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-nArgBP2ΔNSE
Plasmid#74515PurposeExpress GFP-nArgBP2ΔNSE fusion protein in mammalian cells. nArgBP2ΔNSE is the mutant without the neuronal specific exon of nArgBP2.DepositorAvailable SinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tol2-LyzC-RacFRET-U6ac-ctrl guides
Plasmid#168247Purpose"label active Rac; control for neutrophil-specific knockout"DepositorInsertRacFRET
UseZebrafish expressionPromoterLyzCAvailable SinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-HA-Nrxn1α-LNS3
Plasmid#128107PurposeExpress HA tagged Nrxn1α-LNS3DepositorAvailable SinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-HA-Nrxn1α-LNS1
Plasmid#128105PurposeExpress HA tagged Nrxn1α-LNS1DepositorAvailable SinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDL14
Plasmid#106901PurposepDL14 is conditionally replicating plasmid and expresses gam, bet and mutSK622A genes under control of PrhaB promoter for oligo-mediated E. coli chromosome engineering in MMR independent mannerDepositorInsertsgam
bet
mutS
lacI
rhaR
rhaS
ExpressionBacterialMutationLysine 622 was replaced by AlanineAvailable SinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
gRNA_DNMT3a-T1
Plasmid#41821PurposeExpresses a guide RNA (gRNA) to target DNMT3a (T1 target sequence) for genome engineeringDepositorAvailable SinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRExT2A-CT-BmNG
Plasmid#232207PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mNeongreen-3xTy-T2A-BSD-3xTyDepositorInsertmNeongreen
ExpressionBacterialAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRExT2A-CT-PmNG
Plasmid#232201PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mNeongreen-3xTy-T2A-PAC-3xTyDepositorInsertmNeongreen
ExpressionBacterialAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt1(E17K)
Plasmid#86563PurposeExpresses human full-length Akt1(E17K) mutantDepositorAvailable SinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRExT2A-CT-PmSc
Plasmid#232549PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mScarlet-3xTy-T2A-PAC-3xTyDepositorInsertmScarlet
ExpressionBacterialMutationNoneAvailable SinceMarch 3, 2025AvailabilityAcademic Institutions and Nonprofits only