We narrowed to 18,577 results for: MUT

1. pDONR221-hSOX10-G1237A

   Plasmid

   #24733

   Depositor

   William Pavan

   Article

   Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

   Insert

   (sex determining region Y)-box 10 (SOX10 Human)
   

   Use

   Gateway donor vector

   Mutation

   G replaced with A at 1237 position resulting in G…

   Available Since

   May 18, 2010

   Availability

   Academic Institutions and Nonprofits only

2. pDONR221-hSOX10-C1240T

   Plasmid

   #24735

   Depositor

   William Pavan

   Article

   Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

   Insert

   (sex determining region Y)-box 10 (SOX10 Human)
   

   Use

   Gateway donor vector

   Mutation

   C replaced with T at position 1240 resulting in H…

   Available Since

   May 18, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pDONR-hSOX10-G128A

   Plasmid

   #24737

   Depositor

   William Pavan

   Article

   Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

   Insert

   (sex determining region Y)-box 10 (SOX10 Human)
   

   Use

   Gateway donor vector

   Mutation

   G replaced with A at position 128

   Available Since

   May 18, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pDONR221-hSOX10-G1238A

   Plasmid

   #24738

   Depositor

   William Pavan

   Article

   Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

   Insert

   (sex determining region Y)-box 10 (SOX10 Human)
   

   Use

   Gateway donor vector

   Mutation

   G replaced with A at position 1238 resulting in G…

   Available Since

   May 18, 2010

   Availability

   Academic Institutions and Nonprofits only

5. UAS-u(L)Cas9

   Plasmid

   #127385

   Purpose

   Expresses Cas9 at low level

   Depositor

   Michael Boutros

   Article

   Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

   Insert

   uORF(L)-Cas9
   

   Use

   CRISPR

   Expression

   Insect

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-17b_K490clm_E215C

   Plasmid

   #169995

   Purpose

   Kinesin KIF5B K490 cysteine light mutant with E215C mutation for single cysteine labeling

   Depositor

   Ron Vale

   Article

   Niekamp et al Proc Natl Acad Sci U S A. 2019 Mar 5;116

   Insert

   kinesin family member 5B, isoform CRA_a
   

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   C7S, C65A, C168A, C174S, E215C, C294A, C330S, C42…

   Promoter

   T7

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-FLEX-SaCas9-U6-sgRNA

   Plasmid

   #124844

   Purpose

   Vector for Cre-dependent expression of SaCas9

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   SaCas9, gRNA scaffold
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   NLS and NLS-3xHA

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. sq5

   Plasmid

   #24499

   Depositor

   Bruce Conklin

   Article

   Conklin et al Mol Pharmacol. 1996 Oct . 50(4):885-90.

   Insert

   Gs alpha (Gnas Rat)
   

   Tags

   HA (see comments)

   Expression

   Mammalian

   Mutation

   This is Gs alpha with the C-terminal amino acids …

   Available Since

   Aug. 24, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pLKO-CreERT2 stuffer v3

   Plasmid

   #158046

   Purpose

   lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

   Depositor

   Daniel Schramek

   Article

   Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Oct. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pcDNA3-myc-his-BACH1 WT

    Plasmid

    #17642

    Depositor

    Ronny Drapkin

    Article

    Cantor et al Proc Natl Acad Sci U S A. 2004 Feb 24. 1

    Insert

    BACH1 (BRIP1 Human)
    

    Tags

    His and Myc

    Expression

    Mammalian

    Available Since

    April 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO-Cre stuffer v4

    Plasmid

    #158032

    Purpose

    lenti-viral construct with Cre recombinase and U6 driven sgRNA casette with modified tracrRNA (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

    Depositor

    Daniel Schramek

    Article

    Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Sept. 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A

    Plasmid

    #103865

    Purpose

    Catalytically inactive PSPCas13b. RNase activity is inactivated by mutation of catalytic histidine residues in the HEPN domains (H133A/H1058A).

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    PspCas13b
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and HIV NES

    Expression

    Mammalian

    Mutation

    Contains the H133A and H1058A mutations which ina…

    Promoter

    EF1a

    Available Since

    Jan. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-TadA8e-PcrA M6

    Plasmid

    #221229

    Purpose

    Express HACE Editor as a fusion of TadA-8e adenosine deaminase and an optimized PcrA M6 helicase.

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    TadA8e-PcrA M6
    

    Expression

    Mammalian

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. FgH1tUTG

    Plasmid

    #70183

    Purpose

    Inducible expression of guide RNA with fluorescent GFP reporter

    Depositor

    Marco Herold

    Article

    Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

    Insert

    H1-Tet-sgrna cassette
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. gRNA-CasRx_SD1-pSV40-TagBFP

    Plasmid

    #221004

    Purpose

    Transiently express CasRx DR30 repeat with gRNA spacer. Contains SD1 mutation to enhance efficiency. SV40-TagBFP cassette to monitor transfection efficiency. Use BbsI with overhangs Fw-AAAC, Rv-AAAA.

    Depositor

    Benjamin Blencowe, Mikko Taipale

    Article

    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

    Insert

    CasRx 30nt processed direct repeat with SD1 mutation
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    DR1 A>T

    Promoter

    U6

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-SpCas9(H840A)-T2A-EGFP

    Plasmid

    #221231

    Purpose

    Express nCas9 nickase (H840A) with GFP reporter

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    SpCas9(H840A)-T2A-EGFP
    

    Expression

    Mammalian

    Mutation

    H840A

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. VCP(wt)-EGFP

    Plasmid

    #23971

    Depositor

    Nico Dantuma

    Article

    Tresse et al Autophagy. 2010 Feb 22. 6(2):.

    Insert

    Valosin containing protein (VCP Human)
    

    Tags

    6xHis, EGFP, and HA

    Expression

    Mammalian

    Mutation

    none

    Available Since

    Feb. 26, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAS_4xMma PylT_GFP 150TAG 6xHis

    Plasmid

    #140015

    Purpose

    Plasmid with 4xMmaPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    sfGFP
    

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    150TAG

    Promoter

    EF1

    Available Since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. EGFR WT

    Plasmid

    #11011

    Purpose

    Retroviral construct for expressing human EGFR in human cells

    Depositor

    Matthew Meyerson

    Article

    Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

    Insert

    EGFR (EGFR Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAc-sgRNA-Cas9

    Plasmid

    #49330

    Purpose

    Expresses sgRNA and Cas9-Puro in Drosophila S2 cells

    Depositor

    Ji-Long Liu

    Article

    Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

    Inserts

    Cas9
    dU6-sgRNA
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Insect

    Mutation

    Human codon optimised

    Promoter

    Actin-5c and Drosophila U6

    Available Since

    Dec. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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