We narrowed to 18,061 results for: Mut

1. pEGFP_WT-talin1 (1-2541)

   Plasmid

   #166112

   Purpose

   For expression of full-length wild-type mouse talin-1 in mammalian cells. Contains C-terminal EGFP tag.

   Depositor

   Vesa Hytönen

   Article

   Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

   Insert

   WT-Talin-1 (Tln1 Mouse)
   

   Use

   Cmv promoter

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAS_4xMx PylT_GFP 150TAG

   Plasmid

   #140016

   Purpose

   Plasmid with 4xMxPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   150TAG, K46E (Please see depositor comments below)

   Promoter

   EF1

   Available Since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBabe puro HA PIK3CA E545K

   Plasmid

   #12525

   Depositor

   Jean Zhao

   Article

   Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

   Insert

   PIK3CA E545K (PIK3CA Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   E545K. Tumor-derived allele.

   Promoter

   Available Since

   Aug. 18, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. sgTrack-BFP

   Plasmid

   #164273

   Purpose

   Empty sgRNA expression vector with co-expression of BFP reporter

   Depositor

   Charles Rudin

   Article

   Wohlhieter et al Cell Rep. 2020 Dec 1;33(9):108444. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and EFS

   Available Since

   Feb. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBABEpuro-ARAF

   Plasmid

   #51076

   Purpose

   mammalian expression of wt ARAF

   Depositor

   Matthew Meyerson

   Article

   Imielinski et al J Clin Invest. 2014 Apr;124(4):1582-6. d

   Insert

   ARAF (ARAF Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBabe puro Myr HA PIK3CA

   Plasmid

   #12523

   Depositor

   Jean Zhao

   Article

   Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

   Insert

   PIK3CA Myr (PIK3CA Human)
   

   Use

   Retroviral

   Tags

   HA and Myr

   Expression

   Mammalian

   Mutation

   Myristoylation. Constitutively active allele; I14…

   Promoter

   Available Since

   Aug. 18, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-Rab21-Q76L (CA)

   Plasmid

   #83422

   Purpose

   Mouse Rab21 mutant that has defects in GTP hydrolysis (GTP-locked, constitutively active). Generated from pEGFP-Rab21 by site-directed mutagenesis.

   Depositor

   Johanna Ivaska

   Article

   Pellinen et al J Cell Biol. 2006 Jun 5;173(5):767-80.

   Insert

   Rab21 (Rab21 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Residue 76 from Q to L (Q76L: CAG -> CTG)

   Promoter

   CMV

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pXL001-TQM IL13 CAR

   Plasmid

   #154054

   Purpose

   Lentiviral expression of TQM IL13 CAR for CAR-T therapy

   Depositor

   Xiaojun Lian

   Article

   Kim et al Bioact Mater. 2020 May 7;5(3):624-635. d

   Insert

   TQM IL13 CAR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-EGxxFP-Cetn1

   Plasmid

   #50717

   Purpose

   Cetn1 genomic region was placed in pCAG-EGxxFP. Positive control for DSB mediated EGFP reconstitution.

   Depositor

   Masahito Ikawa

   Article

   Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

   Insert

   Cetn1 (Cetn1 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-Rab21-T31N (DN)

    Plasmid

    #83423

    Purpose

    Mouse Rab21 mutant that has defects in the binding of GTP (dominant negative). Generated from pEGFP-Rab21 by site-directed mutagenesis.

    Depositor

    Johanna Ivaska

    Article

    Pellinen et al J Cell Biol. 2006 Jun 5;173(5):767-80.

    Insert

    Rab21 (Rab21 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Residue 31 from T to N (T31N: ACG -> AAC)

    Promoter

    CMV

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRetroX GFP T2A Cre

    Plasmid

    #63704

    Purpose

    Inducible expression of GFP T2A Cre fusion in mammalian cells

    Depositor

    Floris Foijer

    Article

    Foijer et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

    Insert

    T2A Cre
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE promoter

    Available Since

    April 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. DD-DamV133A-tdTomato-LMNB1

    Plasmid

    #159604

    Purpose

    Shield-1 inducible transient mammalian expression of Dam-tdTomato-LMNB1 for DamID, containing the V133A mutant of Dam and a fused tdTomato to monitor fusion protein localization and expression levels.

    Depositor

    Aaron Streets

    Article

    Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.

    Insert

    DD-DamV133A-tdTomato-LMNB1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Dam V133A mutation

    Promoter

    CMV

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. CAV1-K*R-mEGFP

    Plasmid

    #27766

    Depositor

    Ari Helenius

    Article

    Hayer et al J Cell Biol. 2010 Nov 1. 191(3):615-29.

    Insert

    caveolin-1, lysine null mutant (CAV1 Human)
    

    Use

    Tags

    monomeric EGFP

    Expression

    Mammalian

    Mutation

    All lysines mutated to arginines (K*R)

    Promoter

    Available Since

    Aug. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-GFPLaminA-N195K

    Plasmid

    #32708

    Depositor

    Wendy Bickmore

    Article

    Gilchrist et al BMC Cell Biol. 2004 Dec 13;5(1):46.

    Insert

    Lamin A (LMNA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    N195K

    Promoter

    CMV

    Available Since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pCMV-Target-AIDmax

    Plasmid

    #139107

    Purpose

    Mammalian Target-AIDmax expression vector (pSI-626)

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    Bi_partite_NLS-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available Since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. CAG-Flex-TC66T

    Plasmid

    #48331

    Purpose

    Cre-dependant TVA receptor-mCherry fusion with a mutation (66T) in TVA

    Depositor

    Liqun Luo

    Article

    Miyamichi et al Neuron. 2013 Dec 4;80(5):1232-45. doi: 1

    Insert

    TVA receptor mCherry fusion with the 66T mutation
    

    Use

    AAV and Cre/Lox

    Tags

    mCherry fusion to TVA receptor

    Expression

    Mammalian

    Mutation

    66T

    Promoter

    CAG

    Available Since

    Dec. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-GFPLaminA-L85R

    Plasmid

    #32707

    Depositor

    Wendy Bickmore

    Article

    Gilchrist et al BMC Cell Biol. 2004 Dec 13;5(1):46.

    Insert

    Lamin A (LMNA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    L85R

    Promoter

    CMV

    Available Since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pICSL11059

    Plasmid

    #68263

    Purpose

    Level 1 Golden Gate Cassette: Plant hygromycin (with intron) resistance cassette

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Promoter:35s + 5'UTR:Omega + CDS:hptIIwintron + 3'UTR/terminator:35s
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRDA_355

    Plasmid

    #187159

    Purpose

    Lentiviral expression of doxycycline inducible customizable Sp Cas9 sgRNA

    Depositor

    John Doench, David Root

    Article

    Aquilanti et al Neuro Oncol. 2023 Jul 6;25(7):1275-1285.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. ePop

    Plasmid

    #50953

    Purpose

    Expresses E lysis protein that causes in subpopulation death when threshold cell density is reached, resulting in oscillations

    Depositor

    Lingchong You

    Article

    Marguet et al PLoS One. 2010 Jul 30;5(7):e11909. doi:

    Inserts

    E lysis protein
    LuxI
    LuxR
    

    Use

    Tags

    Expression

    Mutation

    Early stop codon introduced in mutated luxR resul…

    Promoter

    Available Since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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