We narrowed to 11,028 results for: HA

1. pFRT-DestFLAGHA_RBPMS2

   Plasmid

   #65748

   Purpose

   constitutively expresses FLAG-HA-tagged RBPMS2 in mammalian cells

   Depositor

   Thomas Tuschl

   Article

   Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

   Insert

   RBPMS2 (RBPMS2 Human)
   

   Tags

   FLAG HA

   Expression

   Mammalian

   Promoter

   CMV, 2x Tet

   Available Since

   June 4, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pBS-AMVpl-γWT-nAchR

   Plasmid

   #97187

   Purpose

   To determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill Co

   Depositor

   Henry Lester

   Article

   Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.

   Insert

   Gamma nicotinic acetylcholine receptor (Chrng Mouse)
   

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-nAChr-Alpha6-mCherry

   Plasmid

   #50480

   Purpose

   this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

   Depositor

   Henry Lester

   Article

   Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

   Insert

   nAChr-alpha6 (Chrna6 Mouse)
   

   Tags

   mCherry fusion in M3-M4 loop (after residue A405)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3.1(+)mGAT1YFP28

   Plasmid

   #41673

   Depositor

   Henry Lester

   Article

   Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

   Insert

   Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
   

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   “Monomerizing” YFP A206K mutation; 28 C-terminal-…

   Promoter

   CMV

   Available Since

   Jan. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFRT-DHX36iso1E335A

   Plasmid

   #159587

   Purpose

   Express N-ter FLAG HA tagged DHX36 isoform 1 with E335A mutation in mammalian cells

   Depositor

   Markus Hafner

   Article

   Sauer et al Nat Commun. 2019 Jun 3;10(1):2421. doi:

   Insert

   DHX36 (DHX36 Human)
   

   Tags

   FLAG HA

   Expression

   Mammalian

   Mutation

   E335A

   Promoter

   CMV

   Available Since

   May 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTWIN1-His6-Ssp-Httex1-43Q

   Plasmid

   #84352

   Purpose

   Expression of the human Huntingtin Exon1 protein containing 43Q in E.coli

   Depositor

   Hilal Lashuel

   Article

   Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

   Insert

   Huntingtin Exon 1 (HTT Human)
   

   Tags

   N-terminal Ssp intein (His-tagged)

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.2/DEST/hCx43-E2HA-stop

   Plasmid

   #40909

   Depositor

   Robin Shaw

   Article

   Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

   Insert

   Connexin 43 internal HA tag (GJA1 Human)
   

   Expression

   Mammalian

   Mutation

   HA tag inserted in second extracellular loop

   Promoter

   CMV

   Available Since

   Nov. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pEGFP-C1 EDD

   Plasmid

   #37190

   Depositor

   Charles Watts, Darren Saunders

   Article

   Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-7

   Insert

   EDD (UBR5 Human)
   

   Tags

   6x His Tag and EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-TRE-ChroME2s-ST-P2A-NLS-mRuby3

   Plasmid

   #170177

   Purpose

   Cation channelrhodopsin ChroME2s targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

   Depositor

   Hillel Adesnik

   Article

   Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

   Insert

   ChroME2s-ST-P2A-NLS-mRuby3
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHLmMBP-6

    Plasmid

    #72346

    Purpose

    Mammalian expression of cytoplasmic mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

    Depositor

    Luca Jovine

    Article

    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

    Type

    Empty backbone

    Tags

    6His-tag, HA-tag, Strep-tag II, and mMBP

    Expression

    Mammalian

    Promoter

    CMV enhancer + chicken beta-actin promoter

    Available Since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDisplay-DNER

    Plasmid

    #51053

    Purpose

    expression of HA-DNER in mammalian cells

    Depositor

    Mineko Kengaku

    Article

    Eiraku et al J Biol Chem. 2002 Jul 12;277(28):25400-7

    Insert

    DNER (Dner Mouse)
    

    Tags

    HA and signal peptide

    Expression

    Mammalian

    Mutation

    without endogenous signal peptide (1-221bp)

    Promoter

    CMV

    Available Since

    Feb. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pBS-KS-attB2-SA(2)-T2A-3X Gal80-Hsp70

    Plasmid

    #62953

    Purpose

    Creating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 1. Gal80 is tagged with FLAG, HA and EGFP.

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    3XGal80-FLAG/HA/EGFP-Hsp70
    

    Available Since

    July 2, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pCK340

    Plasmid

    #192639

    Purpose

    Expresses dSpG and MCP-SoxS(R93A/S101A) on p15A-CmR

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

    Inserts

    Sp.pCas9-dSpG
    BBa_J23107-MCP-SoxS(R93A, S101A)
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    SoxS has R93A and S101A mutations and dSpG has se…

    Promoter

    BBa_J23107 and Sp.pCas9

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSG5 EDD

    Plasmid

    #37191

    Depositor

    Charles Watts, Darren Saunders

    Article

    Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-7

    Insert

    EDD (UBR5 Human)
    

    Tags

    6x His Tag and FLAG

    Expression

    Mammalian

    Mutation

    K503R (please see depositor comments below)

    Promoter

    SV40

    Available Since

    Aug. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLEX-FHH-NRASQ61K-IRES-Puro

    Plasmid

    #120570

    Purpose

    Expresses Flag-HA-6xHIS-N-Ras Q61K fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    Flag-HA-6xHIS

    Expression

    Mammalian

    Mutation

    Q61K

    Promoter

    CMV

    Available Since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBS-KS-attB2-SA(1)-T2A-3X Gal80-Hsp70

    Plasmid

    #62952

    Purpose

    Creating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 1. Gal80 is tagged with FLAG, HA and EGFP.

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    3XGal80-FLAG/HA/EGFP-Hsp70
    

    Available Since

    July 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-Syn-FLEX-rc [SomArchon-GFP]

    Plasmid

    #153533

    Purpose

    AAV-mediated expression of SomArchon-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner.

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

    Insert

    SomArchon-GFP
    

    Use

    AAV

    Tags

    ER2, GFP, and KGC

    Expression

    Mammalian

    Promoter

    Syn

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pF710-pETDuet-1-Hs-delta19-ETFalpha-noHis wt

    Plasmid

    #85111

    Purpose

    expression of recombinant human mature (delta19) ETFalpha wt without tag

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human mature (delta19) ETFalpha (ETFA Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-Syn1-DIO-VGLUT2:miniSOG

    Plasmid

    #214200

    Purpose

    For Cre-dependent expression of VGLUT2:miniSOG (miniSOG is lumen-facing and HA-tagged)

    Depositor

    Thomas Hnasko

    Article

    Steinkellner et al ACS Chem Neurosci. 2021 Feb 17;12(4):626

    Insert

    Slc17a6 tagged with miniSOG and hemagglutinin
    

    Use

    AAV and Cre/Lox

    Tags

    miniSOG (tagged with HA) is fused to the first in…

    Expression

    Mammalian

    Available Since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. THOC1

    Plasmid

    #156081

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    THOC1 (THOC1 Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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