We narrowed to 150,751 results for: ins

1. pQCXIH-PSM-Rb

   Plasmid

   #37106

   Depositor

   Joseph Nevins

   Article

   Angus et al Oncogene. 2012 Jan 16. doi: 10.1038/onc.

   Insert

   Rb (RB1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   aa 379-928 only; large pocket of human Rb with aa…

   Promoter

   CMV

   Available Since

   Sept. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pBC155

   Plasmid

   #202274

   Purpose

   Level 2 for strain-specific barcode DNA tag with bc182 through Tn7 bacterial insertion, Spectinomycin and Streptomycin selectable marker and tagBFP fluorescent marker

   Depositor

   Paul Schulze-Lefert

   Article

   Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

   Insert

   bc182
   

   Expression

   Bacterial

   Available Since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBC114

   Plasmid

   #202276

   Purpose

   Level 2 for strain-specific barcode DNA tag with bc141 through Tn7 bacterial insertion, Tetracyline selectable marker and far-red-FP fluorescent marker mPlum

   Depositor

   Paul Schulze-Lefert

   Article

   Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

   Insert

   bc141
   

   Expression

   Bacterial

   Available Since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJ23119-sgRNA

   Plasmid

   #113654

   Purpose

   sgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.

   Depositor

   Jeffrey Barrick

   Article

   Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

   Insert

   promoter J23119 and sgRNA
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. shCDK4/6(2)

   Plasmid

   #73555

   Purpose

   vector encoding both shRNA against CDK4(2) and shRNA against CDK6(2)

   Depositor

   Gerardo Ferbeyre

   Article

   Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

   Insert

   shRNA against CDK4 + shRNA against CDK6
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

6. Vh-HA-3xFKBP

   Plasmid

   #20222

   Depositor

   Carolyn Bertozzi

   Article

   Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

   Insert

   3xFKBP (FKBP1A Human)
   

   Tags

   HA and Vh

   Expression

   Mammalian

   Available Since

   Feb. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

7. pSEM415 - mosTI unc-119 Pmex-5 - gfp(nls) - tbb-2

   Plasmid

   #182358

   Purpose

   Positive control for mosTI(unc-119). unc-119 rescue and germline nuclear GFP expression after mosTI insertion.

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   GFP
   

   Expression

   Worm

   Promoter

   Pmex-5

   Available Since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Vh-HA-FKBP

   Plasmid

   #20220

   Depositor

   Carolyn Bertozzi

   Article

   Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

   Insert

   FKBP (FKBP1A Human)
   

   Tags

   HA and Vh

   Expression

   Mammalian

   Available Since

   Feb. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

9. PKG I alpha dNPS

   Plasmid

   #16391

   Depositor

   Bernard Weinstein

   Article

   Deguchi et al Mol Cancer Ther. 2002 Aug . 1(10):803-9.

   Insert

   PKG1 alpha (PRKG1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   N-terminal pseudosubstrate domain deletion: delet…

   Available Since

   April 28, 2009

   Availability

   Academic Institutions and Nonprofits only

10. MSP2262

    Plasmid

    #70703

    Purpose

    Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 1: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Inserts

    mammalian codon-optimized Staphylococcus aureus dCas9
    SaCas9 sgRNA (+84)
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Bacterial

    Mutation

    D10A & H557A in SaCas9

    Promoter

    T7

    Available Since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSCM167-Venus-VF-hADAM10(aa220-654)

    Plasmid

    #189560

    Purpose

    Protein expression ADAM10 in mammalian cells. Contains metalloprotease, disintegrin, and cysteine rich domains--amino acids 220-654

    Depositor

    Haian Fu

    Insert

    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    

    Tags

    VF

    Expression

    Mammalian

    Mutation

    contains aa 220-654

    Available Since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNP1 eforRed toehold switch

    Plasmid

    #107353

    Purpose

    Expresses eforRed (an RFP derivative) in Ecoli off a T7 promoter when pNP1 eforRed trigger is co-expressed

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    toehold switch 1 + eforRed
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFUGW-ZipACR-EYFP

    Plasmid

    #88844

    Purpose

    Fast anion channelrhodopsin (current half-decay time 2-4 ms depending on voltage) for time-resolved inhibition of neuronal spiking

    Depositor

    John Spudich

    Article

    Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

    Insert

    ZipACR-NotI-EYFP
    

    Use

    Lentiviral

    Tags

    EYFP

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. IFI16-(192-393)-HinA (pcDNA3-FLAG)

    Plasmid

    #35062

    Depositor

    Cheryl Arrowsmith

    Article

    Liao et al Structure. 2011 Mar 9;19(3):418-29.

    Insert

    IFI16-(192-393)-HinA (IFI16 Human)
    

    Tags

    FLAG and His

    Expression

    Mammalian

    Mutation

    Contains HinA domain: aa 192-393

    Promoter

    CMV

    Available Since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pBabe-PI(WT)-Src(YF)-mCherry

    Plasmid

    #87357

    Purpose

    PI(WT): Photo-inhibitable/blue light sensitive; YF: Y535F const. active mutation

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Inserts

    LOV2
    Src isoform 2 (Src Mouse)
    

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Y535F

    Promoter

    CMV

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. 2XMyc-LRRK2-WD40

    Plasmid

    #25073

    Depositor

    Mark Cookson

    Article

    J Biol Chem. 2008 Jun 13;283(24):16906-1

    Insert

    LRRK2 (LRRK2 Human)
    

    Tags

    2XMyc

    Expression

    Mammalian

    Mutation

    Contains only the WD40 domain aa 2155-2527

    Available Since

    Nov. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

17. IFI16-(515-710)-HinB (pcDNA3-FLAG)

    Plasmid

    #35063

    Depositor

    Cheryl Arrowsmith

    Article

    Liao et al Structure. 2011 Mar 9;19(3):418-29.

    Insert

    IFI16-(515-710)-HinB (IFI16 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains HinB domain: aa 515-710

    Promoter

    CMV

    Available Since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

18. mCherry-Myo10-∆PH

    Plasmid

    #139781

    Purpose

    Detecting Myo10-∆PH by fluorescence microscopy

    Depositor

    Staffan Stromblad

    Article

    Plantard et al J Cell Sci. 2010 Oct 15;123(Pt 20):3525-

    Insert

    Myosin X (MYO10 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Deletion of 3PH domains

    Promoter

    CMV

    Available Since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. AICSDP-83:EZH2-mEGFP

    Plasmid

    #164499

    Purpose

    Homology arms and mEGFP-linker sequence for N-terminus tagging of human EZH2

    Depositor

    Allen Institute for Cell Science

    Insert

    EZH2 Homology Arms with mEGFP-linker (EZH2 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    Feb. 17, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pMTH PKC zeta

    Plasmid

    #8414

    Depositor

    Frederic Mushinski

    Article

    Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

    Insert

    Mouse Protein Kinase C zeta (Prkcz Mouse)
    

    Expression

    Mammalian

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only