179,966 results
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Plasmid#163523PurposeExpresses both eGFP and firefly luciferase in mammalian cellsDepositorInsertFirefly luciferase
UseLuciferaseTagseGFPExpressionBacterial and MammalianAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-Cre-P2A-dTomato (AAV5)
Viral prep#107738-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-hSyn-Cre-P2A-dTomato (#107738). In addition to the viral particles, you will also receive purified pAAV-hSyn-Cre-P2A-dTomato plasmid DNA. hSyn-driven expression of Cre and dTomato (physically separate). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsdTomato (physically separate, not a fusion protein)Available sinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
Human SLC KO Library
Pooled library#132552PurposeKnockout library targeting human SLC proteins.DepositorExpressionMammalianSpeciesHomo sapiensUseCRISPR and LentiviralAvailable sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SpyTag003-MBP
Plasmid#133450PurposeExpresses SpyTag003-MBP (maltose binding protein) in bacterial cytoplasmDepositorInsertSpyTag003-MBP
TagsHis6 and Thrombin cleavage siteExpressionBacterialMutationPeptide tag added to N-terminus of MBP via a GSGE…PromoterT7Available sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP-APEX2-NIK3x
Plasmid#129274PurposeExpression of APEX2 in the nucleolusDepositorInsertGFP-APEX2-NIK3x
UseLentiviralExpressionMammalianPromoterCMV promoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pENTR-PIP-FUCCI
Plasmid#118621PurposeGateway cloning vector for the cell cycle indicator PIP-FUCCIDepositorInsertPIP-FUCCI
UseGateway CloningTagsmCherry and mVenusAvailable sinceApril 13, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLX401-INK4A
Plasmid#121919PurposeDoxycycline-inducible expression of p16-INK4A product of CDKN2ADepositorAvailable sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LC-E75
Bacterial strain#115925PurposeExpression of dCas9 gene under the control of a weak Ptet promoter in the E. coli chromosomeDepositorBacterial resistanceNoneAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CAG-mNeonGreen
Plasmid#99134PurposeAn AAV genome that ubiquitously expresses the fluorescent protein mNeonGreen from the CAG promoterDepositorInsertmNeonGreen
UseAAVExpressionMammalianPromoterCAGAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
EF1a-Cas9-2XNES-2ERT2
Plasmid#120551PurposeExpress Cas9, 2xNES and 2 tandem ERT2 in mammalian cellsDepositorInsertCas9, NES and ERT2
UseLentiviralExpressionMammalianPromoterEF1αAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti HRE-Luc pGK Hygro
Plasmid#118706Purposereporter plasmid HIF-responsive promoter (3xHRE) wt- fused to firefly luciferaseDepositorInsertHRE (HIF-responsive promoter (3xHRE)-luc
UseLentiviralAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a-MH6-EsCas13d
Plasmid#108303PurposeExpresses E. coli codon optimized EsCas13d in the pET28a backbone.DepositorInsertEsCas13d
UseCRISPRTagsmH6 tagExpressionBacterialPromoterlacAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCW57.1 N-term GFP tTA
Plasmid#107551PurposeAll-in-one dox-repressible expression of cDNA with optional N-term GFP tag.DepositorTypeEmpty backboneUseLentiviralTagsGFP (optional)ExpressionMammalianAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA-K-GECO1
Plasmid#105865PurposeMammalian cell expression plasmid for red fluorescent Ca2+ indicator K-GECO1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertK-GECO1
ExpressionMammalianAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a_T7-ARG1
Plasmid#106473PurposeExpresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coliDepositorInsertARG1
ExpressionBacterialPromoterT7Available sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-ZNF598-TEV-3xFLAG
Plasmid#105690PurposeExpresses C terminally 3xFLAG tagged ZNF598 proteinDepositorAvailable sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDEST14-SpyCatcher002
Plasmid#102827PurposeBacterial expression of SpyCatcher002 for covalent coupling to SpyTag002-linked proteinsDepositorInsertSpyCatcher002
TagsGSSGS, His6, and TEV cleavage siteExpressionBacterialPromoterT7Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE
Plasmid#104496PurposeAAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switchDepositorHas serviceAAV1, AAV PHP.eB, and AAV RetrogradeInsertjGCaMP7f
UseAAV and Cre/LoxTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianPromoterCAGAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-ABE6.3
Plasmid#102916Purposeexpresses ABE6.3 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertABE6.3
TagsNLSExpressionMammalianMutationsee manuscriptPromoterCMVAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSimple-miR302/367
Plasmid#98748PurposeEpisomal reprogrammingDepositorAvailable sinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by